A5053763356- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Carcinogens and Genotoxicity Assessment
- Genetic Neurodegenerative Diseases
- Retinal Development and Disorders
- Effects of Radiation Exposure
- RNA Research and Splicing
- Animal Genetics and Reproduction
- Salmonella and Campylobacter epidemiology
- Immune Cell Function and Interaction
- Bacteriophages and microbial interactions
- Neuroscience and Neural Engineering
- RNA Interference and Gene Delivery
- Renal and related cancers
- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- CAR-T cell therapy research
- Microbial infections and disease research
- Spaceflight effects on biology
- Tissue Engineering and Regenerative Medicine
- 3D Printing in Biomedical Research
- Chromosomal and Genetic Variations
- Cancer-related molecular mechanisms research
- Neurogenetic and Muscular Disorders Research
- Probiotics and Fermented Foods
Tomsk National Research Medical Center
2023-2024
Russian Academy of Sciences
2013-2024
Federal Medical-Biological Agency
2018-2024
Federal Research and Clinical Center of Physical-Chemical Medicine named after Y.M. Lopukhin
2016-2023
Engelhardt Institute of Molecular Biology
2023
All-Russian State Scientific Research Institute for Control, Standardization and Certification of Veterinary Preparations
2019-2023
Genetic Technologies (Australia)
2023
Vavilov Institute of General Genetics
2009-2020
Institute of Molecular Genetics
2017
Federal State Budgetary Institution Russian Scientific Center of Roentgenoradiology
2003-2009
Huntington's disease (HD) is an incurable hereditary neurodegenerative disorder, which manifests itself as a loss of GABAergic medium spiny (GABA MS) neurons in the striatum and caused by expansion CAG repeat exon 1 huntingtin gene. There no cure for HD, existing pharmaceutical can only relieve its symptoms. Here, induced pluripotent stem cells were established from patients with low gene, then efficiently differentiated into GABA MS-like (GMSLNs) under defined culture conditions. The...
Abstract Terahertz (THz) radiation was proposed recently for use in various applications, including medical imaging and security scanners. However, there are concerns regarding the possible biological effects of non-ionising electromagnetic THz range on cells. Human embryonic stem cells (hESCs) extremely sensitive to environmental stimuli we therefore utilised this cell model investigate non-thermal irradiation. We studied DNA damage transcriptome responses hESCs exposed narrow-band (2.3...
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Reprogramming of a limited number human cell types has been achieved through ectopic expression four transcription factors to yield induced pluripotent stem (iPS) cells that closely resemble embryonic (ESCs). Here, we determined functional and epigenetic properties iPS generated from umbilical vein endothelial (HUVEC) by conventional method direct reprogramming. Retroviral overexpression resets HUVEC the pluripotency. Human cell-derived (endo-iPS) were similar ESCs in morphology, gene...
Huntington's disease (HD) is a severe autosomal-dominant neurodegenerative disorder caused by mutation within gene, encoding huntingtin protein. Here we have used the induced pluripotent stem cell technology to produce patient-specific terminally differentiated GABA-ergic medium spiny neurons modeling juvenile form of HD (HD76). We shown that calcium signaling dramatically disturbed in HD76 neurons, specifically demonstrating higher levels store-operated and voltage-gated uptakes. However,...
Abstract Background Dozens of transplants generated from pluripotent stem cells are currently in clinical trials. The creation patient-specific iPSCs makes personalized therapy possible due to their main advantage immunotolerance. However, some reports have claimed recently that aberrant gene expression followed by proteome alterations and neoantigen formation can result recognition autologous T-cells. Meanwhile, the possibility NK-cell activation has not been previously considered. This...
<i>Background and aims:</i> Cosmic radiation is one of the main hazards for manned space exploration. Uncertainty in risk estimates crews long-term missions are very high, direct biological measurements necessary. We measured chromosomal aberrations peripheral blood lymphocytes from 33 cosmonauts involved during past 11 years. <i>Methods:</i> Blood were stimulated to grow vitro harvested at their first mitosis. Slides either stained with Giemsa stain dicentrics...
Abstract What strategies do bacteria employ for adaptation to their hosts and are these different varied hosts? To date, many studies on the interaction of bacterium its host have been published. However, global changes in bacterial cell process invasion persistence, remain poorly understood. In this study, we demonstrated phase transition avian pathogen Mycoplasma gallisepticum upon various types eukaryotic cells (human, chicken, mouse) which was stable during several passages after...
DNA-intercalated motifs (iMs) are facile scaffolds for the design of various pH-responsive nanomachines, including biocompatible pH sensors. First, DNA sensors relied on complex intermolecular scaffolds. Here, we used a simple unimolecular dual-labeled iM scaffold and minimized it by replacing redundant loop nucleosides with abasic or alkyl linkers. These modifications improved thermal stability increased rates its pH-induced conformational transitions. The best effects were obtained upon...
Genome stability of human embryonic stem cells (hESC) is an important issue because even minor genetic alterations can negatively impact cell functionality and safety. The incorrect repair DNA double-stranded breaks (DSBs) the ultimate cause formation chromosomal aberrations. Using G2 radiosensitivity assay, we analyzed aberrations in pluripotent somatic cells. chromatid exchange aberration rates hESCs increased manifold 2 hours after irradiation as compared with their differentiated...
G-quadruplex (G4) sites in the human genome frequently colocalize with CCCTC-binding factor (CTCF)-bound CpG islands (CGIs). We aimed to clarify role of G4s CTCF positioning. Molecular modeling data suggested direct interactions, so we performed vitro binding assays quadruplex-forming sequences from CGIs genome. bound Kd values similar that control duplex, while respective i-motifs exhibited no affinity for CTCF. Using ChIP-qPCR assays, showed G4-stabilizing ligands enhance occupancy at a...
About 15% of patients with parkinsonism have a hereditary form Parkinson’s disease (PD). Studies on the early stages PD pathogenesis are challenging due to lack relevant models. The most promising ones models based dopaminergic neurons (DAns) differentiated from induced pluripotent stem cells (iPSCs) forms PD. This work describes highly efficient 2D protocol for obtaining DAns iPSCs. is rather simple, comparable in efficiency previously published protocols, and does not require viral...
The pluripotency of newly developed human induced pluripotent stem cells (iPSCs) is usually characterized by physiological parameters; i.e., their ability to maintain the undifferentiated state and differentiate into derivatives 3 germ layers. Nevertheless, a molecular comparison physiologically normal iPSCs "gold standard" pluripotency, embryonic (ESCs), often reveals set genes with different expression and/or methylation patterns in ESCs. To evaluate contribution reprogramming process,...
A bstract Background Prostate adenocarcinoma (PRAD) is the second leading cause of cancer-related deaths in men. High variability DNA methylation and a high rate large genomic rearrangements are often observed PRAD. Results To investigate reasons for such variance, we integrated methylation, RNA-seq, copy number alterations datasets from The Cancer Genome Atlas (TCGA), focusing on PRAD, employed weighted gene co-expression network analysis (WGCNA). Our results show that only single cluster...
Abstract Ovarian cancer often develops resistance to conventional therapies, hampering their effectiveness. Here, using ex vivo paired ovarian ascites obtained before and after chemotherapy in vitro therapy-induced secretomes, we show that molecules secreted by cells upon therapy promote cisplatin enhance DNA damage repair recipient cells. Even a short-term incubation of chemonaive with secretomes induces changes resembling those are observed chemoresistant patient-derived tumor long-term...
The aim of the study was to obtain induced pluripotent stem cells (iPSCs) from patients with various forms Parkinson's disease (PD), and create on this basis a platform for studying pathogenesis at molecular cellular level development protocol cell differentiation.Materials Methods.iPSCs were derived cultured skin fibroblasts, taken five PD (PARK8, PARK2, GBA-associated sporadic forms), reprogrammed help lentiviral vectors Sendai virus.The obtained iPSCs clones stage embryonic bodies and,...
The retinal pigment epithelium is a monolayer of pigmented, hexagonal cells connected by tight junctions. These compose part the outer blood-retina barrier, protect eye from excessive light, have important secretory functions, and support function photoreceptors, ensuring coordination variety regulatory mechanisms. It degeneration that root cause many degenerative diseases. search for reliable cell sources transplantation extreme urgency. Pluripotent stem (embryonic or induced pluripotent)...
Abstract Parthenogenetic species of Caucasian rock lizards the genus Darevksia are important evidence for reticulate evolution and speciation by hybridization in vertebrates. Female‐only lineages formed through interspecific have been discovered many groups. Nevertheless, critical mechanisms oogenesis specifics meiosis that provide long‐term stability parthenogenetic still unknown. Here we report cytogenetic characteristics somatic karyotypes meiotic prophase I nuclei diploid Darevskia...