A5057771677- Wind and Air Flow Studies
- Aerodynamics and Fluid Dynamics Research
- Building Energy and Comfort Optimization
- RNA and protein synthesis mechanisms
- Fluid Dynamics and Turbulent Flows
- Rare-earth and actinide compounds
- Liver Disease Diagnosis and Treatment
- Magnetic Properties of Alloys
- CRISPR and Genetic Engineering
- Engineering Applied Research
- Meteorological Phenomena and Simulations
- Advanced optical system design
- Radiative Heat Transfer Studies
- Synthetic Organic Chemistry Methods
- Cancer and Skin Lesions
- Advanced biosensing and bioanalysis techniques
- Hydrogen Storage and Materials
- RNA Research and Splicing
- Chemical Synthesis and Reactions
- Iron-based superconductors research
- Supercapacitor Materials and Fabrication
- Peptidase Inhibition and Analysis
- Infection Control and Ventilation
- Cell Adhesion Molecules Research
- Fluid Dynamics and Vibration Analysis
Nippon Medical School
2021-2024
University of Tsukuba
2013-2023
Kyoto University
2018-2023
Central Research Institute of Electric Power Industry
2014-2022
Weatherford College
2022
Kubota (Japan)
2022
Waseda University
2001-2021
Keio University
2019
Kyushu University
2003-2017
Iwate University
2016
Myocardial infarction (MI) results in the generation of dead cells infarcted area. These are swiftly removed by phagocytes to minimize inflammation and limit expansion damaged However, types molecules responsible for engulfment area remain largely unknown. In this study, we demonstrated that cardiac myofibroblasts, which execute tissue fibrosis producing extracellular matrix proteins, efficiently engulf cells. Furthermore, identified a population myofibroblasts appears heart after MI humans...
Synthetic biological circuits are designed to regulate gene expressions control cell function. To date, these often use DNA-delivery methods, which may lead random genomic integration. lower this risk, an all RNA system, in the circuit and delivery method constituted of components, is preferred. However, construction complexed using RNA-delivered devices living cells has remained a challenge. Here we show synthetic mRNA-delivered with RNA-binding proteins for logic computation mammalian...
Commercially available Computational Fluid Dynamics (CFD) software have been applied in indoor environmental design recent years, but the prediction accuracy depends on an understanding of fluid dynamics fundamentals, setting appropriate boundary and numerical conditions. This study aims to provide practical modelling information related problematic areas CFD applications air conditioning ventilation, through a series benchmark tests reported results. Six commercial codes were evaluated...
Abstract Molecular left-right (L-R) asymmetry is established at the node of mouse embryo as a result sensing leftward fluid flow by immotile cilia perinodal crown cells and consequent degradation Dand5 mRNA on left side. We here examined how induces decay. found that first 200 nucleotides in 3′ untranslated region (3′-UTR) are necessary sufficient for left-sided decay to mediate response 3′-UTR reporter transgene Ca 2+ , cation channel Pkd2, RNA-binding protein Bicc1 their regulation...
The aim of this retrospective multicenter study was to clarify the antifibrotic effect and long-term outcome sodium glucose cotransporter 2 inhibitors (SGLT2-Is) in patients with nonalcoholic fatty liver disease (NAFLD) complicated by type diabetes mellitus (T2DM). Of 1262 consecutive T2DM who recently received SGLT2-Is, 202 NAFLD had been receiving SGLT2-Is for more than 48 weeks were subjected analysis. Furthermore, 109 on SGLT2-I therapy 3 years at time analysis assessed effects SGLT2-Is....
Recent indoor environmental design is requested to create comfortable and safety space in addition the maximizing energy conservation performance buildings. In this point of view, it important enhance prediction accuracy quality stage. Commercial Computational Fluid Dynamics (CFD) software practically applied recent years but CFD simulation depends on understanding for fundamentals fluid dynamics setting appropriate boundary numerical conditions as well. The series study aimed provide with...
Commercial Computational Fluid Dynamics (CFD) software is practically applied in indoor environmental design recent years but the prediction accuracy of CFD simulation depends on understanding for fundamentals fluid dynamics and setting appropriate boundary numerical conditions as well. Additionally, deeper to a specific problem regarding environment also requested. The series this study aimed provide with practical information such problematic areas related applications environment, air...
Abstract Translational modulation based on RNA-binding proteins can be used to construct artificial gene circuits, but capable of regulating translation efficiently and orthogonally remain scarce. Here we report CARTRIDGE (Cas-Responsive Regulation Integratable into Diverse Gene control) repurpose Cas as translational modulators in mammalian cells. We demonstrate that a set repress or activate the designed mRNAs contain Cas-binding RNA motif 5’-UTR. By linking multiple Cas-mediated...
It has been shown that Porphyromonas gingivalis 381, a suspected periodontopathogen, possesses fimbriae on its cell surface. The organism is also known to produce proteases which can degrade the host surface matrix proteins. In this study, we investigated effect of protease binding purified P. cultured fibroblasts or A hydrolyze benzoyl-L-arginine p-nitroanilide was obtained from 381 cells by sonication in phosphate-buffered 0.2% Triton X-100 and column chromatography. molecular size...
D-Amino acid oxidase (DAO) was extracted and purified from cultured mycelia of Fusarium solani M-0718 (FERM P-2688). The enzyme able to oxidatively deaminate cephalosporin C 7-beta-(5-carboxy-5-oxopentanamido)cephalosporanic acid. Ninety-eight amino residues the F. DAO were determined by sequence analysis 9 peptides derived Acromobacter protease I digests protein. Complementary DNAs encoding isolated cDNA library hybridization with synthetic oligonucleotide probes corresponding partial...
Background— The balance between apoptosis and proliferation of vascular smooth muscle cells (VSMCs) is believed to contribute the remodeling process. Cyclic AMP response element–binding protein (CREB) a critical transcription factor for survival neuronal T lymphocytes. However, role CREB in blood vessels incompletely characterized. Methods Results— Nuclear staining with Hoechst 33258 or propidium iodine showed an increase apoptotic activation caspase-3 VSMCs infected adenovirus expressing...
Summary FlhG , a MinD homolog and an ATPase is known to mediate the formation of single polar flagellum V ibrio alginolyticus together with FlhF . work antagonistically, promoting flagellar assembly inhibiting it. Here, we demonstrate that purified exhibits low basal ATP ase activity. As activity can be activated D 171 A residue substitution enhances its sevenfold. ‐ localizes strongly at cell pole severely inhibits motility flagellation, whereas K 31 K36Q mutants, which are defective in...
Computational Fluid Dynamics (CFD) has become practical design tool for indoor environment recent years and the application cases have been increasing. Though improvement of prediction accuracy CFD is needed in connection with upgrade quality Heating, Ventilation air-conditioning (HVAC) system, simulation depends on understanding fundamentals fluid dynamics setting appropriate boundary numerical conditions as well. Additionally, deeper to a specific problem regarding also required. The...
The lack of available genetic modules is a fundamental issue in mammalian synthetic biology. Especially, the variety parts for translational control are limited. Here we report new set mRNA-based switches by engineering RNA-binding proteins (RBPs) and RBP-binding RNA motifs (aptamers) that perform strong repression. We redesigned with scaffolds improved efficiency repression to target RBPs. Using previously reported mRNA switches, demonstrated orthogonality regulation was ensured among five...
Background: Although sodium-glucose cotransporter 2 inhibitors (SGLT2-Is) improve not only glycemic control but also liver inflammation and fatty changes in patients with non-alcoholic disease (NAFLD) type diabetes mellitus (T2DM), its sustainability effect on fibrosis have remained unclear. The current study aimed to clarify the effects of 48-week SGLT2-I therapy inflammation, changes, NAFLD T2DM. Methods: This evaluated NAFLD, including assessed via transient elastography, 56 who received...
Total syntheses of α-bisabol-1-one, curcumene, curcuphenol and elvirol have been accomplished via 1,4-conjugate addition intact aldehydes to vinyl ketones followed by an intramolecular aldol condensation.
Abstract Myocardial infarction (MI) is an ischaemic heart condition caused by the occlusion of coronary arteries. Following MI, lactic acid from anaerobic glycolysis increases and infiltrating immune cells produce severe inflammation, which leads to acidosis in heart. However, physiological implication this pH reduction remains largely unknown. T-cell death-associated gene 8 (TDAG8) a proton-sensing G protein-coupled receptor found on cardiac macrophages that recognise extracellular protons....
Migration of vascular smooth muscle cells (VSMCs) contributes to formation stenotic lesions such as atherosclerosis and restenosis after angioplasty. Previous studies have demonstrated that tumor necrosis factor-alpha (TNF-alpha) is a potent migration factor for VSMCs. cAMP-response element-binding protein (CREB) the stimulus-induced transcription activates target genes c-fos interleukin-6. We examined whether CREB involved in TNF-alpha-induced VSMC migration.TNF-alpha induced...