A5020446076- Geophysical Methods and Applications
- Landslides and related hazards
- Geophysical and Geoelectrical Methods
- Seismic Waves and Analysis
- Single-cell and spatial transcriptomics
- Cancer Genomics and Diagnostics
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Soil and Unsaturated Flow
- RNA modifications and cancer
- Neurogenesis and neuroplasticity mechanisms
- Cancer Cells and Metastasis
- Dam Engineering and Safety
- Advanced Antenna and Metasurface Technologies
- Pluripotent Stem Cells Research
- Glioma Diagnosis and Treatment
- Image Processing and 3D Reconstruction
- 3D Surveying and Cultural Heritage
- Renal and related cancers
- Ovarian cancer diagnosis and treatment
- Soil Geostatistics and Mapping
- Soil Moisture and Remote Sensing
- Growth Hormone and Insulin-like Growth Factors
- Electromagnetic Compatibility and Measurements
- MicroRNA in disease regulation
Università della Svizzera italiana
2024
Institute of Oncology Research
2024
European Institute of Oncology
2018-2021
National Research Council
2017-2019
Institute of Biomedical Technologies
2017-2019
Istituti di Ricovero e Cura a Carattere Scientifico
2018-2019
National Research Council - Institute of Methodologies for Environmental Analysis
2012-2018
University of Milan
2018
European School of Molecular Medicine
2018
National Academies of Sciences, Engineering, and Medicine
2010-2015
Outer radial glia (oRG) emerge as cortical progenitor cells that support the development of an enlarged outer subventricular zone (oSVZ) and expansion neocortex. The in vitro generation oRG is essential to investigate underlying mechanisms human neocortical expansion. By activating STAT3 signaling pathway using leukemia inhibitory factor (LIF), which not expressed guided organoids, we define a organoid differentiation method from pluripotent stem (hPSCs) recapitulates pool into oSVZ. oSVZ...
Abstract High Grade Serous Ovarian cancer (HGSOC) is a major unmet need in oncology, due to its precocious dissemination and the lack of meaningful human models for investigation disease pathogenesis patient-specific manner. To overcome this roadblock, we present new method isolate grow single cells directly from patients’ metastatic ascites, establishing conditions propagating them as 3D cultures that refer cell-derived ovarian spheroids (sMOCS). By cell RNA sequencing (scRNAseq) define...
Abstract Background High-grade serous ovarian cancer (HGSOC) is a major unmet need in oncology. The remaining uncertainty on its originating tissue has hampered the discovery of molecular oncogenic pathways and development effective therapies. Methods We used an approach based retention tumors DNA methylation trace (OriPrint) that distinguishes two putative tissues origin HGSOC, fimbrial (FI) surface epithelia (OSE), to stratify HGSOC by several clustering methods, both linear non-linear....
Abstract. This paper reports the results of a geoelectrical survey carried out to investigate Montaguto earth-flow, located in southern Apennines (Campania Region, Italy). The aim was reconstruct geometry landslide body, improve knowledge on geological setting and indirectly test effectiveness drainage system. Although electrical resistivity contrasts images were not very pronounced, due lithological characteristic outcropping lithotypes, it possible observe presence both lateral vertical...
Transcriptional regulators are crucial in adipocyte differentiation. We now show that the homeodomain-containing transcription factor Prep1 is a repressor of adipogenic differentiation since its down-regulation (DR) both ex vivo bone marrow-derived mesenchymal stromal cells (MSC) and vitro 3T3-L1 preadipocytes significantly increases their ability. acts at stage preceding activation machinery because DR makes more prone to even absence inducers. expands DNA binding landscape C/EBPβ (CCAAT...
Ground penetrating radar (GPR) and electrical resistivity tomography (ERT) are typical geophysical techniques applied into archaeological context thanks to their capability study variations of physical attributes buried objects in the soil causing electric electromagnetic contrasts compared behaviour surrounding environment. Unfortunately sometimes these difficult define for several reasons including strong attenuation signals introduced soil, limited depth low resolution investigation...
A prototype system for time-lapse acquisition of 2D electrical resistivity tomography (ERT) and time domain reflectometry (TDR) measurements was installed in a test site affected by landslide Basilicata region (southern Italy). The aim the is to monitor real-time rainwater infiltration into soil obtain information about variation water content first layers subsoil possible influence this on activity. rain gauge placed gives rainfall intensity frequency suggests interval. preliminary results...
Abstract High Grade Serous Ovarian cancer (HGSOC) is a major unmet need in oncology, due to its precocious dissemination and the lack of meaningful human models for investigation disease pathogenesis patient-specific manner. To overcome this roadblock, we present new method isolate grow single cells directly from patients’ ascites, establishing conditions propagating them as single-cell derived ovarian organoids (scOCOs). By cell RNA sequencing (scRNAseq) define cellular composition...
Molecular mechanisms governing cell fate decision events in bone marrow mesenchymal stromal cells (MSC) are still poorly understood. Herein, we investigated the homeobox gene Prep1 as a candidate regulatory molecule, by adopting hypomorphic mice model to investigate effects of downregulation, using vitro and vivo assays, including innovative single RNA sequencing technology. Taken together, our findings indicate that low levels associated enhanced adipogenesis concomitant reduced...
The generation of human pluripotent stem cell (hPSC)-derived brain organoids is continuously refined, enhancing their reproducibility and complexity. Here, we present a guided differentiation protocol for generating cortical forebrain cortico-pericyte (CP)assembloids composed robust outer radial glia (oRG) population an expanded subventricular zone (oSVZ). We describe the steps to generate hPSC-derived (COs), pericytes, CP assembloids. Moreover, outline procedures characterize by...
Summary In the last ten years, time-lapse electrical resistivity tomography technique has been applied to monitor temporal and spatial changes of values in different environmental natural processes like saline intrusion, aquifer recharge, water content change, etc. The growing use this is mainly due significant technological improvements occurred recent years. Indeed, introduction new static multi-channel systems allowed reduction acquisition time, remote control system monitoring variation...
Summary The ArchaeoGeophysical techniques are based on the investigation of buried cultural structures and surrounding materials in archaeological framework. Unfortunately, geophysical contrast between features soils sometimes difficult to define due problems sensitivity resolution both related characteristic subsoil methods. An experimental activity has been performed Hydrogeosite laboratory, a full large scale controlled site consisting pool shape 230m3 where remains have installed....
A permanent monitoring system for time–lapse acquisition of 2D Electrical Resistivity Tomography (ERT) and Time Domain Reflectometry (TDR) measurements was installed in a test site affected by landslide Basilicata region (southern Italy). Time-lapse allows us to monitor real time the variation resistivity values first layers subsoil, closely connected with water content potentially associated reactivation landslide. weather station gives information about rainfall intensity frequency...
Abstract High grade serous ovarian cancer (HGSOC) is a major unmet need in oncology. The persistent uncertainty on its originating tissue has contributed to hamper the discovery of oncogenic pathways and effective therapies. Here we define DNA methylation print that distinguishes human fimbrial (FI) surface epithelia (OSE) develop robust epigenetic cell-of-origin tracer stratifies HGSOC FI-and OSE-originated tumors across all available cohorts. We translate this origin-based stratification...
e17063 Background: The still persistent uncertainty in the identification of cell origin high grade serous ovarian cancer (HGSOC), with two candidate originating tissues identified distal tract fallopian tube (FI) and surface epithelium ovary (OSE), has hampered clinically relevant molecular features for this disease to be targeted therapy. This resulted only a negligible improvement patient’s care since introduction carboplatin-based treatments. Methods: To solve issue, here we show an...