A5038755096- Genomics and Chromatin Dynamics
- Monoclonal and Polyclonal Antibodies Research
- Single-cell and spatial transcriptomics
- Telomeres, Telomerase, and Senescence
- Protein purification and stability
- Glycosylation and Glycoproteins Research
- RNA modifications and cancer
- Advanced Biosensing Techniques and Applications
- Epigenetics and DNA Methylation
- T-cell and B-cell Immunology
- Ubiquitin and proteasome pathways
- Viral Infectious Diseases and Gene Expression in Insects
- Cancer-related Molecular Pathways
- Muscle Physiology and Disorders
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Immune cells in cancer
- CRISPR and Genetic Engineering
- RNA regulation and disease
- RNA Research and Splicing
- Acne and Rosacea Treatments and Effects
- Gene expression and cancer classification
- PI3K/AKT/mTOR signaling in cancer
- Transgenic Plants and Applications
Kyushu University
2008-2024
Cancer Research UK
2013-2021
University of Cambridge
2013-2021
Shiga University of Medical Science
2020-2021
Tottori University
2013-2016
Japan Society for the Promotion of Science
2013-2016
Senescence is a stress-responsive form of stable cell cycle exit. Senescent cells have distinct gene expression profile, which often accompanied by the spatial redistribution heterochromatin into senescence-associated heterochromatic foci (SAHFs). Studying key component nuclear lamina lamin B1 (LMNB1), we report dynamic alterations in its genomic profile and their implications for SAHF formation regulation during senescence. Genome-wide mapping reveals that LMNB1 depleted senescence,...
Abstract Senescent cells interact with the surrounding microenvironment achieving diverse functional outcomes. We have recently identified that NOTCH1 can drive ‘lateral induction’ of a unique senescence phenotype in adjacent by specifically upregulating NOTCH ligand JAG1. Here we show signalling modulate chromatin structure autonomously and non-autonomously. In addition to senescence-associated heterochromatic foci (SAHF), oncogenic RAS-induced senescent (RIS) exhibit massive increase...
The downstream functions of the DNA binding tumor suppressor p53 vary depending on cellular context, and persistent activation has recently been implicated in suppression senescence. However, genome-wide information about p53-target gene regulation derived mostly from acute genotoxic conditions. Using ChIP-seq expression data, we have found distinct profiles between acutely activated (through damage) chronically (in senescent or pro-apoptotic conditions) p53. Compared to classical 'acute'...
Abstract Cell states are regulated by the response of signaling pathways to receptor ligand-binding and intercellular interactions. High-resolution imaging has been attempted explore dynamics these processes and, recently, multiplexed profiled cell achieving a comprehensive acquisition spatial protein information from cells. However, specificity antibodies is still compromised when visualizing activated signals. Here, we develop Precise Emission Canceling Antibodies (PECAbs) that have...
Electrolyzed reduced water (ERW) has attracted much attention because of its therapeutic effects. In the present study, a new culture medium, which we designated Water was developed to elucidate effects ERW on lifespan Caenorhabditis elegans. Wild-type C. elegans had significantly shorter in medium than conventional S medium. However, worms cultured ERW-Water exhibited extended (from 11% 41%) compared with ultrapure water-Water There no difference between lifespans ERW-S and water-S...
Abstract Single‐cell RNA‐sequencing analysis is one of the most effective tools for understanding specific cellular states. The use single cells or pooled in RNA‐seq requires isolation from a tissue culture. Although trypsin more recently cold‐active protease (CAP) has been used cell dissociation, extent to which gene expression changes are suppressed not clarified. To this end, we conducted detailed profiling enzyme‐dependent mouse skeletal muscle progenitor cells, focusing on enzyme...
Histone H3mm18 is a non-allelic H3 variant expressed in skeletal muscle and brain mice. However, its function has remained enigmatic. We found that incorporated into chromatin cells with low efficiency, as compared to H3.3. determined the structures of nucleosome core particle (NCP) containing by cryo-electron microscopy, which revealed entry/exit DNA regions are drastically disordered NCP. Consistently, NCP substantially unstable vitro. The forced expression mouse myoblast C2C12 markedly...
We have established an in vitro immunization protocol whereby human peripheral blood mononuclear cells (PBMCs) are initially treated with L-leucyl-L-leucine methyl ester (LLME) and subsequently sensitized antigen the presence of interleukin (IL)-2, IL-4, adjuvant. This resulted production antigen-specific antibodies. PBMCs potentiated to react exogenous antigens upon treatment LLME. using this system investigate immunomodulatory activity additives. In present study, we aimed evaluate...
The site‐specific excision of a target DNA sequence for genetic knockout or lineage tracing is powerful tool investigating biological systems. Currently, recombinases ( SSR s), such as Cre Flp recombination cassettes, have been successfully excised inverted by single to regulate transgene expression. However, the use might restrict complex control gene This study investigated potential expanding multiple regulation transgenes using three different integrase systems TP 901‐1, R4, and Bxb1)....
Human monoclonal antibodies have great potential for use in the treatment of various diseases. We established an vitro immunization protocol inducing antigen-specific antibody production from human peripheral blood mononuclear cells (PBMCs). In protocol, PBMCs are pretreated with L-leucyl-L-leucine methyl ester (LLME) to remove suppressive cells, and sensitized cultured a soluble antigen presence IL-2, IL-4 muramyl dipeptide 8 d, then is produced. this study, we examined novel possibility...
An in vitro immunization protocol using human peripheral blood mononuclear cells (PBMC) was developed to generate antigen-specific antibodies. Monoclonal antibodies have great potential, and particular, efficient acquirement of monoclonal against membrane proteins provides advantages. In this study, we tried a antibody the high affinity IgE receptor, Fc(epsilon)RI(alpha), method combining phage display. Heavy light chain variable region genes were obtained from PBMC immunized with...
ABSTRACT Senescent cells interact with the surrounding microenvironment achieving diverse functional outcomes. In addition to autocrine and paracrine signalling mediated by factors of senescence-associated secretory phenotype, we have recently identified that NOTCH1 can drive ‘lateral induction’ a unique form senescence in adjacent through specific induction NOTCH ligand JAG1. Here show modulate chromatin structure both autonomously non-autonomously. heterochromatic foci (SAHF), oncogenic...
<title>Abstract</title> Transcription factor-bound chromatin structures regulate cell lineages in multicellular organisms. Single-cell epigenomics has the potential to reveal lineage determination on structure, but methodology is still development. Here, we develop single-cell combinatorial-indexing multi-target Chromatin Integration Labeling followed by sequencing (sci-mtChIL-seq) as a multi-epigenomics approach, which enables simultaneous analysis of both RNA polymerase II binding and...
ABSTRACT Abnormalities in spermatogenesis, a fundamental component of male reproductive function, can cause infertility. Somatic cells constituting the testis microenvironment are essential for controlling normal spermatogenesis. Although testicular somatic thought to sense and respond germ ensure proper details this signaling mechanism unknown. Here, we investigated cell dynamics tissue lacking spermatogenesis using mice with deletion testis‐specific histone H3 variant gene H3t . Testicular...
ABSTRACT Spatial omics enables comprehensive mapping of cell types and states in their spatial context, providing profound insights into cellular communication tissue organization. However, analyzing large sections, especially crucial for clinical applications, remains a significant challenge due to the computational demands current image processing methods. To overcome these limitations, we developed MEGA-FISH, flexible, GPU-accelerated Python framework optimized large-scale analysis....
Abstract Recent advances in omics studies have enabled analysis at the single-cell level; however, methods for analyzing whole cell of large organs and tissues remain challenging. Here, we developed a method named tsChIL to understand diverse cellular dynamics tissue level using high-depth epigenomic data. allowed single section could reproducibly acquire profiles from several types tissues, based on distribution target states, morphology, number cells. The proposed independent evaluation...