A5041011253- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Lipid Membrane Structure and Behavior
- Photosynthetic Processes and Mechanisms
- Protein Structure and Dynamics
- Enzyme Structure and Function
- Monoclonal and Polyclonal Antibodies Research
- Escherichia coli research studies
- RNA Interference and Gene Delivery
- Cellular transport and secretion
- Enzyme Production and Characterization
- Pancreatic function and diabetes
- ATP Synthase and ATPases Research
- Mitochondrial Function and Pathology
- Solid State Laser Technologies
- Genomics and Phylogenetic Studies
- Ion channel regulation and function
- Peptidase Inhibition and Analysis
- Legume Nitrogen Fixing Symbiosis
- Mass Spectrometry Techniques and Applications
- Enzyme Catalysis and Immobilization
- Advanced Proteomics Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Protein Hydrolysis and Bioactive Peptides
University of Hohenheim
2016-2025
Gesundheitsamt
2024-2025
Center for Systems Biology Dresden
2024
Thermo Fisher Scientific (Germany)
2023
Thermo Fisher Scientific (Canada)
2023
Karlsruhe Institute of Technology
1991-2022
École Nationale Supérieure de Chimie, de Biologie et de Physique
2018
Institut des Sciences Moléculaires
2018
Heraeus (Germany)
2016
Institut für angewandte Photonik
2016
The growing trend toward high-throughput proteomics demands rapid liquid chromatography-mass spectrometry (LC-MS) cycles that limit the available time to gather large numbers of MS/MS fragmentation spectra required for identification. Orbitrap analyzers scale performance with acquisition and necessarily sacrifice sensitivity resolving power deliver higher rates. We developed a new mass spectrometer combines mass-resolving quadrupole, Orbitrap, novel Asymmetric Track Lossless (Astral)...
Escherichia coli B cells were sensitized to ionophore A23187 by polymyxin nonapeptide, and the induced magnesium potassium ion fluxes studied. Combined treatment permeabilized cytoplasmic membrane of E. in an ion-specific manner allowed manipulation intracellular Mg2+ content from outside. A23187-induced efflux or influx was dependent on free concentration gradient between outside inside pH gradient. Most bound, whereas only 1 2 mM solution cellular sap.
The quantitative analysis of protein mixtures is pivotal for the understanding variations in proteome living systems. Therefore, approaches have been recently devised that generally allow relative peptides and proteins. Here we present proof concept new metal-coded affinity tag (MeCAT) technique, which allowed determination A macrocyclic metal chelate complex (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA)) loaded with different lanthanides (metal(III) ions) was essential...
YidC is a newly defined translocase component that mediates the insertion of proteins into membrane bilayer. How functions in process not known. In this study, we report Sec-independent Pf3 coat protein requires specifically for translocation step. Using photocrosslinking techniques and ribosome-bound derivatives with an extended carboxyl-terminal region, found transmembrane region physically interacts bacterial signal recognition particle Ffh component. We also find pathway, strongly only...
The membrane insertion of the Sec-independent M13 Procoat protein in bacteria requires electrochemical potential and integral YidC. We show here that YidC is involved translocation but not targeting protein, because we found was partitioned into absence can function also to promote mutants insert independently potential, proving effect depletion due a dissipation potential. absolutely required for Sec-dependent long periplasmic loop mutant which region has been extended from 20 194 residues....
Abstract The growing trend towards high-throughput proteomics demands rapid liquid chromatography-mass spectrometry (LC-MS) cycles that limit the available time to gather large numbers of MS/MS fragmentation spectra required for identification. Orbitrap analyzers scale performance with acquisition time, and necessarily sacrifice sensitivity resolving power deliver higher rates. We developed a new mass spectrometer combines quadrupole, novel Asymmetric Track Lossless (Astral) analyzer. hybrid...
Tryptophans have a high affinity for the membrane-water interface and been suggested to play role in determining topology of membrane proteins. We investigated this potential experimentally, using mutants single-spanning Pf3 coat protein, whose transmembrane topologies are sensitive small changes amino acid sequence. Mutants were constructed with varying numbers tryptophans flanking region translocation was assessed by an vitro translation/translocation system. Translocation into Escherichia...
SecA, the dimeric ATPase subunit of bacterial protein translocase, catalyses translocation during ATP-driven membrane cycling at SecYEG. We now show that SecA protomer comprises two structural modules: N-domain, containing nucleotide binding sites NBD1 and NBD2, regulatory C-domain. The C-domain binds to N-domain in each another form dimers. is sufficient for single rounds ATP hydrolysis. Multiple turnovers require both NBD2 site acting cis a conserved sequence operating trans. This...
Assembly of several inner membrane proteins—leader peptidase (Lep), a Lep derivative (Lep-inv) that inserts with an inverted topology compared the wild-type protein, phage M13 procoat and (H1-procoat) hydrophobic core signal peptide replaced by stretch from first transmembrane segment in Lep—has been studied vitro Escherichia coli strains are conditional for expression either 54 homologue (Ffh) or 4.5S RNA, which two components E. recognition particle (SRP), SecE, essential component...
Gene 8 of bacteriophage M13 codes for procoat, the precursor its major coat protein.Gene has been cloned into a plasmid and mutagenized.W e have isolated mutants this gene in which procoat is synthesized but not processed to protein.We now describe leader region at positions -6, -3, -1 with respect peptidase cleavage site.These are quite conserved among peptides various pre-proteins.Each these mutant procoats normal rate inserts correctly plasma membrane, as judged by accessibility protease...