A5009427443- Alzheimer's disease research and treatments
- Prion Diseases and Protein Misfolding
- thermodynamics and calorimetric analyses
- Trace Elements in Health
- Neurological diseases and metabolism
- Amyotrophic Lateral Sclerosis Research
- Protein Interaction Studies and Fluorescence Analysis
- RNA Research and Splicing
- Bacterial Genetics and Biotechnology
- DNA and Nucleic Acid Chemistry
- Lipid Membrane Structure and Behavior
- RNA and protein synthesis mechanisms
- Endoplasmic Reticulum Stress and Disease
- Catalytic C–H Functionalization Methods
- Protein Structure and Dynamics
- Drug Transport and Resistance Mechanisms
- N-Heterocyclic Carbenes in Organic and Inorganic Chemistry
- Protein purification and stability
- Catalytic Cross-Coupling Reactions
- Mitochondrial Function and Pathology
- Advanced Glycation End Products research
- Proteins in Food Systems
- Biotin and Related Studies
- Enzyme Structure and Function
- Molecular Sensors and Ion Detection
China Pharmaceutical University
2024-2025
Wuhan University
2014-2024
Nanjing University of Science and Technology
2023-2024
City University of Hong Kong, Shenzhen Research Institute
2021-2024
State Key Laboratory of Natural Medicine
2024
Gannan Normal University
2023
University of British Columbia
2022
Nanjing Forestry University
2022
State Key Laboratory of Virology
2019-2021
Renmin Hospital of Wuhan University
2021
The 5′-cap structure is a distinct feature of eukaryotic mRNAs, and viruses generally modify the 5′-end viral RNAs to mimic cellular mRNA structure, which important for RNA stability, protein translation immune escape. SARS coronavirus (SARS-CoV) encodes two S-adenosyl-L-methionine (SAM)-dependent methyltransferases (MTase) sequentially methylate cap at guanosine-N7 ribose 2′-O positions, catalyzed by nsp14 N7-MTase nsp16 2′-O-MTase, respectively. A unique SARS-CoV that requires...
A hallmark of a group neurodegenerative diseases such as Alzheimer disease is the formation neurofibrillary tangles, which are principally composed bundles filaments formed by microtubule-associated protein Tau. Clarifying how natively unstructured Tau forms abnormal aggregates central importance for elucidating etiology these diseases. There considerable evidence showing that zinc, an essential element highly concentrated in brain, linked to development or progression Herein, using...
Intraneuronal neurofibrillary tangles composed of Tau aggregates have been widely accepted as an important pathological hallmark Alzheimer's disease. A current therapeutic avenue for treating disease is aimed at inhibiting accumulation with small molecules such natural flavonoids. Liquid-liquid phase separation (LLPS) can lead to its aggregation, and then be degraded by autophagy. However, it unclear whether flavonoids modulate the formation phase-separated droplets or promote autophagy...
The membrane insertion of the Sec-independent M13 Procoat protein in bacteria requires electrochemical potential and integral YidC. We show here that YidC is involved translocation but not targeting protein, because we found was partitioned into absence can function also to promote mutants insert independently potential, proving effect depletion due a dissipation potential. absolutely required for Sec-dependent long periplasmic loop mutant which region has been extended from 20 194 residues....
Background Amyloid fibrils associated with neurodegenerative diseases can be considered biologically relevant failures of cellular quality control mechanisms. It is known that in vivo human Tau protein, prion and copper, zinc superoxide dismutase (SOD1) have the tendency to form fibril deposits a variety tissues they are different diseases, while rabbit protein hen egg white lysozyme do not readily unlikely cause diseases. In this study, we investigated contrasting effect macromolecular...
The N-heterocyclic carbene catalyzed [3 + 3] annulation of indolin-2-imines and bromoenals was developed to give dihydropyridinone-fused indoles in good high yields, which were transformed α-carbolines with different 2-subsituents by a process dehydrogenation, tosylation, palladium C–C or C–N coupling reaction.
The early noninvasive diagnosis of Alzheimer's disease targeted β-amyloid (Aβ) plaques or Tau tangles is a major challenge because the coshared β-sheet structure target. In contrast to tailoring probes specific amyloids, here, we showed that near-infrared (NIR) environment-sensitive probe 18 could fluorescently discriminate Aβ and from artificial aggregates pathological change in brain tissue. biological evaluation demonstrated substantial fluorescence enhancement, large blueshift emission...
Abstract Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease. Misfolded Cu, Zn-superoxide dismutase (SOD1) has been linked to both familial and sporadic ALS. SOD1 fibrils formed in vitro share toxic properties with ALS inclusions. Here we produced cytotoxic amyloid from full-length apo human under reducing conditions determined the atomic structure using cryo-EM. The fibril consists of single protofilament left-handed helix. core exhibits serpentine fold comprising N-terminal...
YidC was previously discovered to play a critical role for the insertion of Sec-independent M13 procoat and Pf3 coat phage proteins into Escherichia coli inner membrane. To determine whether there is an absolute requirement membrane protein any endogenous E. proteins, we investigated few representative proteins. We found that subunits F(0) sector F(1)F(0)ATP synthase SecE SecYEG translocase are highly dependent on insertion, based protease mapping immunoblot analysis. dependency does not...
Previously, the role of YidC in membrane protein biogenesis F(0) sector Escherichia coli F(1)F(0) ATP synthase was investigated. Whereas subunits a and c were strictly dependent on for insertion, subunit b required efficient insertion (Yi, L., Jiang, F., Chen, M., Cain, B., Bolhuis, A., Dalbey, R. E. (2003) Biochemistry 42, 10537-10544). In this paper, we investigated other components energetics that are assembly subunits. We show here Sec translocase signal recognition particle (SRP)...
The oxidative refolding of reduced, denatured hen egg white lysozyme in the presence a mixed macromolecular crowding agent containing both bovine serum albumin (BSA) and polysaccharide has been studied from physiological point view. When total concentration is 100 g/liter, which weight ratio BSA to dextran 70 1:9, yield after for 4 h under this condition increases 24% compared with that 16% 70. A remarkable increase by Ficoll also observed. Further folding kinetics analyses show these two...
The folding of protein, an important process for protein to fulfill normal functions, takes place in crowded physiological environments. α-Lactalbumin, as a model system protein-folding studies, has been used extensively because it can form stable molten globule states under range conditions. Here we report that the crowding agents Ficoll 70, dextran and polyethylene glycol (PEG) 2000 have different effects on structural stability human α-lactalbumin (HLA) represented by transition state: 70...
Abstract Amyotrophic lateral sclerosis (ALS) involves the abnormal posttranslational modifications and fibrillization of copper, zinc superoxide dismutase (SOD1) TDP-43. However, how SOD1-catalyzed reaction product hydrogen peroxide affects amyloid formation SOD1 TDP-43 remains elusory. 90% ALS cases are sporadic remaining familial ALS. In this paper, we demonstrate that H 2 O at pathological concentrations triggers wild-type both in vitro SH-SY5Y cells. Using an anti-dimedone antibody...
The bifunctional N-heterocyclic carbene catalyzed [3 + 4] annulation of enals and 5-alkenyl thiazolones was developed, giving the corresponding thiazole-fused ε-lactones in high yields with excellent diastereoselectivties enantioselectivities. ε-lactone could be isomerized to spirocyclic thiazolone–cyclopentanone without erosion enantioselectivity.
Novel D-π-A-π-D probes were investigated for the detection of Aβ plaques and NFTs. The displayed remarkable optical properties, DADNIR-2 possessed high affinity towards Tau aggregates (Kd = 0.41 nM 1.04 nM, respectively) with certain selectivity. could penetrate BBB label in vivo.