A5077087004- Evolution and Genetic Dynamics
- RNA Research and Splicing
- Genetics, Aging, and Longevity in Model Organisms
- RNA modifications and cancer
- Mitochondrial Function and Pathology
- Evolutionary Game Theory and Cooperation
- RNA and protein synthesis mechanisms
- Renal Transplantation Outcomes and Treatments
- Diet and metabolism studies
- Microbial Metabolic Engineering and Bioproduction
- Extracellular vesicles in disease
- Eicosanoids and Hypertension Pharmacology
- Pregnancy and Medication Impact
- Renal Diseases and Glomerulopathies
- Genetic and Kidney Cyst Diseases
- Renal and related cancers
- Birth, Development, and Health
- Gene Regulatory Network Analysis
- Mathematical and Theoretical Epidemiology and Ecology Models
- Pregnancy and preeclampsia studies
- Physiological and biochemical adaptations
- Electrolyte and hormonal disorders
- Cardiovascular Disease and Adiposity
- Spaceflight effects on biology
- Cardiac Ischemia and Reperfusion
University Hospital Cologne
2019-2024
University of Cologne
2018-2024
Cologne Excellence Cluster on Cellular Stress Responses in Aging Associated Diseases
2019-2024
Evanston Hospital
2022
Cologne Business School
2021
Friedrich Schiller University Jena
2010-2016
Max Planck Institute for Chemical Ecology
2013
University of Würzburg
1979
Bacteria that have adapted to nutrient-rich, stable environments are typically characterized by reduced genomes. The loss of biosynthetic genes frequently renders these lineages auxotroph, hinging their survival on an environmental uptake certain metabolites. evolutionary forces drive this genome degradation, however, remain elusive. Our analysis 949 metabolic networks revealed auxotrophies likely highly prevalent in both symbiotic and free-living bacteria. To unravel whether selective...
Abstract Due to its small size Drosophila melanogaster can conveniently be used in screening experiments for anatomical brain mutants. A simple method has been designed which allows process up 20 identifiable flies as a single preparation standard histology routine. Anatomical mutants are very frequent. Over 60 were obtained from the inspection of about 3000 brains. So far genetic variations structure fall into 4 classes: (1) “low fidelity” brains less precisely built; (2) “brain shape” with...
Bacterial communities are taxonomically highly diverse, yet the mechanisms that maintain this diversity remain poorly understood. We hypothesized an obligate and mutual exchange of metabolites, as is very common among bacterial cells, could stabilize different genotypes within microbial communities. To test this, we developed a cellular automaton to model interactions six empirically characterized differ in their ability propensity produce amino acids. By systematically varying intrinsic...
Kidney transplantation is the preferred renal replacement therapy available. Yet, long-term transplant survival unsatisfactory, partially due to insufficient possibilities of longitudinal monitoring and understanding biological processes after transplantation. Small urinary extracellular vesicles (suEVs) - as a non-invasive source information were collected from 22 living donors recipients. Unbiased proteomic analysis revealed temporal patterns suEV protein signature cellular involved in...
Significance Statement AKI is a major clinical complication leading to high mortality, but intensive research over the past decades has not led targeted preventive or therapeutic measures. In rodent models, caloric restriction (CR) and transient hypoxia significantly prevent recent comparative transcriptome analysis of murine kidneys identified kynureninase (KYNU) as shared downstream target. The present work shows that KYNU strongly contributes CR-mediated protection key player in de novo...
Significance Statement Preconditioning strategies, such as caloric restriction and hypoxic preconditioning, show strongly protective effects in animal models of AKI, researchers hope exploration these strategies might provide insights into translating powerful interventions to the clinical setting. However, molecular mechanisms underlying beneficial short-term application preconditioning have remained elusive. The authors used RNA-sequencing transcriptome profiling compare transcriptional...
AATF is a central regulator of the cellular outcome upon p53 activation, finding that has primarily been attributed to its function as transcription factor. Recent data showed essential for ribosome biogenesis and plays role in rRNA maturation. implicated fulfil this through direct interaction with was identified several RNA-interactome capture experiments. Here, we provide first comprehensive analysis RNA bound by using CLIP-sequencing. Interestingly, approach shows predominant binding 45S...
Significance Statement RNA-binding proteins (RBPs) are crucial regulators of cellular biology, and recent evidence suggests that regulation RBPs modulate both RNA stability translation may have a profound effect on the proteome. However, little is known about upon clinically relevant changes microenvironment. The authors used high-throughput approaches to study RNA‐binding proteome in differentiated tubular epithelial cells exposed hypoxia. They identified number novel (suggesting these be...
Mycophenolate Mofetil (MMF) has an established role as a therapeutic agent in childhood nephrotic syndrome. While other immunosuppressants have been shown to positively affect podocytes, direct effects of MMF on podocytes remain largely unknown. The present study examines the MMF's active component Mycophenolic Acid (MPA) transcriptome and investigates its biological significance. We performed transcriptomics cultured murine exposed MPA generate hypotheses podocyte-specific MPA. Accordingly,...
Chronic Kidney Disease (CKD), a global health burden, is strongly associated with age-related renal function decline, hypertension, and diabetes, which are all frequent consequences of obesity. Despite extensive studies, the mechanisms determining susceptibility to CKD remain insufficiently understood. Clinical evidence together prior studies from our group showed that perinatal metabolic disorders after intrauterine growth restriction or maternal obesity adversely affect kidney structure...
Abstract The extracellular matrix (ECM) is a pivotal three-dimensional network crucial for tissue organization, cellular communication, and fundamental processes, where collagens are the major chemical entity in amount. ECM deregulation directly involved with several pathologies, such as tumour growth invasiveness, atherosclerosis, diabetic nephropathy. Mutations von Hippel-Lindau suppressor (pVHL) cause VHL syndrome, multi-tumour syndrome commonly associated clear cell renal carcinoma...
ABSTRACT AATF is a central regulator of the cellular outcome upon p53 activation, finding that has primarily been attributed to its function as transcription factor. Recent data showed essential for ribosome biogenesis and plays role in rRNA maturation. implicated fulfil this through direct interaction with was identified several RNA-interactome capture experiments. Here, we provide first comprehensive analysis RNA bound by using CLIP-sequencing. Interestingly, approach shows predominant...
Abstract Caloric Restriction (CR) extends lifespan and augments cellular stress-resistance from yeast to primates, making CR an attractive strategy for organ protection in the clinic. Translation of patients is complex, due problems regarding adherence, feasibility safety concerns frail patients. Novel tailored dietary regimens, which modulate composition macro- micronutrients rather than reducing calorie intake promise similar protective effects increased translatability. However, a direct...
Abstract JADE family proteins (JADE1/2/3) have been implicated in diverse cellular functions and signaling pathways ranging from WNT cell cycle control to death complex transcriptional regulation through histone acetyl-transferase complexes. show a high degree of sequence similarity share two PHD zinc finger domains. JADE1 interacts with cilia-associated has cilia-related genetic disorders kidney phenotypes. However, the function widely expressed at molecular level is still elusive. Here we...
Abstract Kidney transplantation is the preferred renal replacement therapy available. Yet, biological processes during and after kidney how they translate into overall functional graft outcome are insufficiently understood. Recent developments in field of extracellular vesicle research allow deeper exploitation this non-invasive source. We separated small urinary vesicles (suEVs) throughout course living donor transplantation. SuEVs were collected longitudinally from both recipient 22...