A5054936194- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- Microtubule and mitosis dynamics
- Glycosylation and Glycoproteins Research
- Cellular transport and secretion
- Plant tissue culture and regeneration
- Porphyrin Metabolism and Disorders
Universität Hamburg
2017-2021
To produce seeds, flowering plants need to specify somatic cells undergo meiosis. Here, we reveal a regulatory cascade that controls the entry into meiosis starting with group of redundantly acting cyclin-dependent kinase (CDK) inhibitors KIP-RELATED PROTEIN (KRP) class. KRPs function by restricting CDKA;1-dependent inactivation Arabidopsis Retinoblastoma homolog RBR1. In rbr1 and krp triple mutants, designated meiocytes several mitotic divisions, resulting in formation supernumerary give...
To follow the dynamics of meiosis in model plant Arabidopsis, we have established a live cell imaging setup to observe male meiocytes. Our method is based on concomitant visualization microtubules (MTs) and meiotic cohesin subunit that allows following five cellular parameters: shape, MT array, nucleus position, nucleolus chromatin condensation. We find states these parameters are not randomly associated identify 11 states, referred as landmarks, which occur much more frequently than closely...
Significance Accurate chromosome segregation at mitosis and meiosis is crucial to prevent genome instability, birth defect, cancer. Accordingly, separase, the protease that triggers distribution, tightly regulated by a direct inhibitor, securin. However, securin has not been identified, neither functionnally nor sequence similarity, in other clades fungi animals. This raised doubts about conservation of this mechanism branches eukaryotes. Here, we identify characterize plants. Despite...
Abstract Meiosis is essential for sexual reproduction and key to the generation of genetic diversity. To reveal robustness meiocyte differentiation progression through meiosis, we have here established a live cell imaging setup follow dynamics individual male meiocytes in Arabidopsis. Our method based on concomitant visualization microtubules meiotic cohesion subunit that allowed following five cellular parameters: shape, nucleus position, nucleolus chromatin condensation microtubule array....
Live cell imaging has tremendously promoted our understanding of cellular and subcellular processes such as division. Here, we present a step-by-step protocol for robust easy-to-use live approach to study male meiosis in the plant Arabidopsis thaliana recently established. Our method relies on concomitant analysis two reporter genes that highlight chromosome configurations microtubule dynamics. In combination, these allowed discrimination five parameters: shape, array, nucleus position,...
Abstract Chromosome distribution at anaphase of mitosis and meiosis is triggered by separase, an evolutionarily conserved protease. Separase must be tightly regulated to prevent the untimely release chromatid cohesion disastrous chromosome defects. Securin key inhibitor separase in animals fungi, but has not been identified other eukaryotic lineages. Here, we PATRONUS1 PATRONUS2 (PANS1 PANS2) as Arabidopsis homologues securin. Disruption PANS1 known lead premature separation chromosomes...