A5089693058- Microtubule and mitosis dynamics
- Chromosomal and Genetic Variations
- Porphyrin Metabolism and Disorders
- CRISPR and Genetic Engineering
- Cellular transport and secretion
- Glycosylation and Glycoproteins Research
- Photosynthetic Processes and Mechanisms
- Insect symbiosis and bacterial influences
AgroParisTech
2019-2021
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2021
Université Paris-Saclay
2019-2021
Institut Jean-Pierre Bourgin
2019-2021
Significance Accurate chromosome segregation at mitosis and meiosis is crucial to prevent genome instability, birth defect, cancer. Accordingly, separase, the protease that triggers distribution, tightly regulated by a direct inhibitor, securin. However, securin has not been identified, neither functionnally nor sequence similarity, in other clades fungi animals. This raised doubts about conservation of this mechanism branches eukaryotes. Here, we identify characterize plants. Despite...
CRISPR-Cas9 has proven to be highly valuable for genome editing in plants, including the model plant Physcomitrium patens. However, fact that most of events produced using native Cas9 nuclease correspond small insertions and deletions is a limitation. base editors enable targeted mutation single nucleotides eukaryotic genomes therefore overcome this Here, we report two programmable base-editing systems induce precise cytosine or adenine conversions P. Using editors, site-specific single-base...
Abstract Chromosome distribution at anaphase of mitosis and meiosis is triggered by separase, an evolutionarily conserved protease. Separase must be tightly regulated to prevent the untimely release chromatid cohesion disastrous chromosome defects. Securin key inhibitor separase in animals fungi, but has not been identified other eukaryotic lineages. Here, we PATRONUS1 PATRONUS2 (PANS1 PANS2) as Arabidopsis homologues securin. Disruption PANS1 known lead premature separation chromosomes...