A5084727295- Glaucoma and retinal disorders
- Retinal Diseases and Treatments
- Corneal surgery and disorders
- Ophthalmology and Eye Disorders
- Retinal Development and Disorders
- Ocular Surface and Contact Lens
- Corneal Surgery and Treatments
- Retinal Imaging and Analysis
- Fibroblast Growth Factor Research
- Retinal and Macular Surgery
- Ocular Diseases and Behçet’s Syndrome
- Intraocular Surgery and Lenses
- Cerebral Venous Sinus Thrombosis
- Protein Kinase Regulation and GTPase Signaling
- Proteoglycans and glycosaminoglycans research
- Ocular Infections and Treatments
- Neurogenesis and neuroplasticity mechanisms
- Melanoma and MAPK Pathways
- Phytochemical Studies and Bioactivities
- Advanced Battery Technologies Research
- Analytical Chemistry and Chromatography
- Hippo pathway signaling and YAP/TAZ
- Phytochemistry and Bioactive Compounds
- Epigenetics and DNA Methylation
- Wnt/β-catenin signaling in development and cancer
Kumamoto University
2016-2025
Kumamoto University Hospital
2012-2022
Japan Science and Technology Agency
2010-2018
University of Fukui
2018
The University of Tokyo
1995-2017
Kissei Pharmaceutical (Japan)
2014-2017
Duke University
2008-2015
Kumamoto City Hospital
2015
Futaba clinic
2014
Institute of Navigation
2013
This multicenter retrospective study compared the surgical outcomes of Baerveldt glaucoma implant (BGI) surgery with those Ahmed valve (AGV) in patients neovascular (NVG). The included NVG aged ≥ 20 years who had undergone BGI (223 eyes) or AGV (146 between April 1, 2012 and December 31, 2021 across 10 clinical centers Japan. Surgical success failure was primary outcome measure this study. We defined as a reduction < 20% pre-operative intraocular pressure (IOP) criterion A (IOP > 21 mmHg), B...
The proliferation and differentiation of neural precursor cells are mutually exclusive during brain development. Despite its importance for cell self renewal, the molecular linkage between these two events has remained unclear. Fibroblast growth factor 2 (FGF2) promotes concurrently inhibits their differentiation, suggesting a cross talk signaling pathways downstream FGF receptor. We demonstrate that FGF2 through phosphatidylinositol 3 kinase activation inactivates glycogen synthase 3beta...
Significance The lack of intravital imaging axonal transport mitochondria in the living mammalian CNS precludes characterization dynamics diseased and aged CNS. Here we report minimally invasive multiphoton mouse retinal ganglion cells that offers sequential time-lapse images transported a single axon with submicrometer resolution. We show highly dynamic vivo under physiological conditions characterize disturbances mitochondrial glaucoma model age-related changes transport. Our method is...
Abstract Ripasudil hydrochloride hydrate (K-115), a specific Rho-associated coiled-coil containing protein kinase (ROCK) inhibitor, was the first ophthalmic solution developed for treatment of glaucoma and ocular hypertension in Japan. Topical administration K-115 decreased intraocular pressure (IOP) increased outflow facility rabbits. This study evaluated effect on monkey trabecular meshwork (TM) cells Schlemm’s canal endothelial (SCE) cells. induced retraction rounding cell bodies as well...
Abstract Purpose To investigate the intra‐ocular pressure ( IOP )‐lowering effects and safety of 0.4% ripasudil (K‐115), a Rho kinase inhibitor, twice daily for 52 weeks, in patients with open‐angle glaucoma or ocular hypertension OHT ). Methods In this multicentre, prospective, open‐label study, 388 primary glaucoma, exfoliation were enrolled 354 them subdivided into four cohorts (monotherapy, 173; additive therapy to prostaglandin analogs, 62; β ‐blockers, 60; fixed combination drugs, 59)....
We conducted a series of phase 1 clinical trials to elucidate the efficacy and safety selective Rho kinase inhibitor K-115 as candidate drug for treatment glaucoma. report intraocular pressure (IOP)-lowering effects based on our results.To study IOP-lowering topical administration inhibitor, K-115, in healthy male adult volunteers.Randomized, placebo-controlled, double-masked, group comparison trial.In initial single-instillation trial, 50 volunteers were subdivided into groups treated with...
Ripasudil hydrochloride hydrate (K-115), a novel rho kinase inhibitor, provides statistically significant intraocular pressure (IOP)-lowering effects and has tolerable safety profile. However, no studies have evaluated ripasudil combined with β-blockers prostaglandin analogues.To evaluate the additive IOP-lowering of ripasudil, 0.4%, timolol, 0.5%, or latanoprost, 0.005%, in patients primary open-angle glaucoma ocular hypertension.We conducted 2, multicenter, randomized, double-masked,...
Abstract Purpose To investigate the intra‐ocular pressure ( IOP )‐lowering effects of a selective Rho kinase inhibitor, ripasudil (K‐115), over 24 hr in patients with primary open‐angle glaucoma POAG ) or ocular hypertension OHT ). Methods In this multicenter, prospective, randomized, open‐label, 3‐period, Latin‐square crossover clinical study, 28 whose level was 21 mmHg higher were subdivided into three groups. Each patient treated placebo and concentrations 0.2 0.4%, at 9:00 21:00 on day 1...
Thermodynamic property measurements for actinides in the LiCl‐KCl/Cd system have been obtained as part of development an electrochemical pyropartitioning process to remove from PUREX waste residues. Electrochemical potential and distribution ratios were employed obtain standard Gibbs free energies formation actinide chlorides LiCl‐KCl, activity coefficients reduced Cd, relative neodymium. The temperature‐dependent U, Np, Pu , when T is K. energy was −548.7 kJ/mol at 450°C. U LiCl‐KCl ranged...
To evaluate cytokine and growth factor levels in the aqueous humor patients with open-angle glaucoma (OAG).Kumamoto University Hospital, Kumamoto, Japan.Cross-sectional cohort study.Aqueous samples were collected from cataract cases OAG cases. Aqueous of cytokines factors determined by multiplex immunoassay. The data analyzed using Tukey-Kramer honestly-significant-difference test multiple regression analyses.The study evaluated 52 73 In cases, mean interleukin (IL)-6, IL-8, monocyte...
Purpose.: To investigate the effect of a specific inhibitor Rho-associated protein kinase, Y-27632, on monkey Schlemm's canal endothelial (SCE) cells. Methods.: SCE cells were isolated from cynomolgus eyes. The effects Y-27632 aqueous outflow facility evaluated using enucleated eyes and constant-pressure perfusion system. barrier function confluent SCE-cell monolayer was by measuring transendothelial electrical resistance (TEER) fluorescein permeability. Y-27632–induced changes in...
To elucidate the impact of phacoemulsification on aqueous monocyte chemoattractant protein-1 (MCP-1) levels, and identify its cell origin.For clinical study, humor samples were collected before after surgery (17.0 ± 4.0 months postoperatively) from 21 cataract cases that underwent intraocular lens (IOL) implantation. Aqueous MCP-1 levels determined using a multiplex immunoassay. For animal experiments, rabbits (± IOL). nonoperated eyes operated eyes, immunoassays performed. Eyes analyzed by...
To investigate levels of biomarkers Alzheimer's disease in the aqueous humor patients with open-angle glaucoma (OAG).Aqueous samples were collected from 38 cataracts, 20 POAG, and 32 exfoliation glaucoma. Aqueous apolipoprotein (Apo) AI, ApoCIII, ApoE, transthyretin (TTR), complement factor H, C3, α2-macroglobulin (α2M) determined by multiplex bead immunoassay. Clinical data obtained medical charts.Age sex did not differ significantly among groups. POAG had higher Apo TTR, α2M compared...
Purpose: To investigate the roles of Yes-associated protein (YAP)/transcriptional co-activator with PDZ-binding motif (TAZ), major effector molecules Hippo pathway, in TGF-β2–mediated conjunctival fibrosis. Methods: Primary human fibroblasts were treated TGF-β2. The expression YAP/TAZ was examined by Western blot analyses and immunocytochemistry. fibrotic proteins genes evaluated quantitative real-time PCR, respectively. effects on changes knockdown experiments inhibitor, verteporfin....
Purpose To analyze the effects of factors on aqueous humor proinflammatory cytokine and growth factor levels in patients with uveitic glaucoma (UG). Methods In this cross-sectional study, we enrolled 143 participants: 1) UG (n = 39); 2) primary open-angle (POAG) 36); 3) cataract surgery patients, as a comparative group 68). Aqueous samples were obtained at start surgery. determined using multiplex immunoassay (xMAP Human Cytokine/Chemokine Panel I). Results cases, mean interleukin (IL)-6,...
Arylative and alkenylative ring-opening reactions of a [2.2.1]- or [3.2.1]oxabicyclic alkene with Grignard reagent take place in the presence catalytic amount iron(III) chloride stoichiometric TMEDA to produce highly substituted 3-cyclohexen-1-ol 3-cyclohepten-1-ol good yield high regio- stereoselectivity.
The identification of a new class potent and selective ROCK-II inhibitors is presented. Compound 5 (SR-3677) had an IC50 ∼3 nM in enzyme cell based assays off-target hit rate 1.4% against 353 kinases, inhibited only 3 out 70 nonkinase enzymes receptors. Pharmacology studies showed that was efficacious both, increasing ex vivo aqueous humor outflow porcine eyes inhibiting myosin light chain phosphorylation.
We investigated the involvement of RhoA/Rho kinase (ROCK) signal transduction pathway in dexamethasone (DEX)-induced changes aqueous outflow.Using trabecular meshwork (TM) and Schlemm's canal endothelial (SCE) cells, RhoA activation was evaluated with a pull-down assay myosin light chain phosphorylation by Western blot analysis. Outflow facility measured perfused porcine anterior segment organ cultures treated DEX and/or Y-27632, selective ROCK inhibitor. The barrier function cultured cells...
To investigate the rate and risk factors for neovascular glaucoma (NVG) after vitrectomy in proliferative diabetic retinopathy (PDR).Five hundred twelve patients (512 eyes) with PDR who underwent between January 1, 2003 June 30, 2009 at Kumamoto University Hospital, Japan, were retrospectively evaluated. Postoperative NVG was defined as neovascularization anterior segment intraocular pressure (IOP) ≥ 22 mm Hg vitrectomy. Kaplan-Meier survival analysis applied to calculate of Risk identified...