A5082435520- Salmonella and Campylobacter epidemiology
- Vibrio bacteria research studies
- Bacterial biofilms and quorum sensing
- Escherichia coli research studies
- Bacterial Genetics and Biotechnology
- Trace Elements in Health
- Antibiotic Resistance in Bacteria
- Redox biology and oxidative stress
- Bacteriophages and microbial interactions
- Sulfur Compounds in Biology
- Vitamin C and Antioxidants Research
- Immune Response and Inflammation
- Bacterial Infections and Vaccines
- Cancer Research and Treatments
- Microbial Fuel Cells and Bioremediation
Karolinska Institutet
2010-2016
Medizinische Hochschule Hannover
2010-2011
Bacterial genotoxins, produced by several Gram-negative bacteria, induce DNA damage in the target cells. While responses induced host cells have been extensively studied vitro, role of these effectors during course infection remains poorly characterized. To address this issue, we assessed effects Salmonella enterica genotoxin, known as typhoid toxin, vivo models murine infection. Immunocompetent mice were infected with isogenic S. enterica, serovar Typhimurium (S. Typhimurium) strains,...
Cellulose, a 1,4 beta-glucan polysaccharide, is produced by variety of organisms including bacteria. Although the production cellulose has high biological, ecological and economical impact, regulatory mechanisms biosynthesis are mostly unknown. Family eight cellulases regularly associated with operons in bacteria; however, their function poorly characterized. In this study, we analysed role cellulase BcsZ encoded bcsABZC operon Salmonella enterica serovar Typhimurium (S. Typhimurium) biofilm...
Cytolethal-distending toxins (CDTs) belong to a family of DNA damage inducing exotoxins that are produced by several Gram-negative bacteria. Salmonella enterica serovar Typhi expresses its CDT (named as Typhoid toxin) only in the Salmonella-containing vacuole (SCV) infected cells, which requires export for cell intoxication. The mechanisms secretion, release extracellular space and uptake bystander cells poorly understood. We have addressed these issues using recombinant S. Typhimurium...
Production of reactive oxygen species represents a fundamental innate defense against microbes in diversity host organisms. Oxidative stress, amongst others, converts peptidyl and free methionine to mixture methionine-S- (Met-S-SO) methionine-R-sulfoxides (Met-R-SO). To cope with such oxidative damage, sulfoxide reductases MsrA MsrB are known reduce MetSOs, the former being specific for S-form latter R-form. However, at present role pathogenesis intracellular bacterial pathogens has not been...
Vaccination represents an important instrument to control typhoid fever in humans and protects mice from lethal infection with mouse pathogenic serovars of Salmonella species. Mixed infections tagged can be used combination probabilistic models describe the dynamics process. Here we mixed oral strains identify bottlenecks process naïve vaccinated mice. We established a next generation sequencing based method characterize composition which offers fast reliable characterise genome-tagged...
ABSTRACT At present, Salmonella is considered to express two peroxiredoxin-type peroxidases, TsaA and AhpC. Here we describe an additional peroxiredoxin, Tpx, in enterica show that a single tpx mutant susceptible exogenous hydrogen peroxide (H 2 O ), it has reduced capacity degrade H compared the ahpCF tsaA mutants, its growth affected activated macrophages. These results suggest Tpx contributes significantly sophisticated defense system pathogen evolved survive oxidative stress.
In Salmonella enterica serovar Typhimurium (S. Typhimurium), biofilm-formation is controlled by the cytoplasmic intracellular small-molecular second messenger cyclic 3′, 5′-di- guanosine monophosphate (c-di-GMP) through activities of GGDEF and EAL domain proteins. Here we describe that deleting either dsbA or dsbB, respectively encoding a periplasmic protein disulfide oxidase membrane oxidoreductase, resulted in increased on solid medium. This biofilm-formation, defined as red, dry rough...
CsgD and cyclic-3',5'-di-guanylate are key regulators of biofilm formation in Salmonella enterica serovar Typhimurium. Our results show that polynucleotide phosphorylase NlpI oppositely altered expression CsgD. Polynucleotide also had opposite effects on the yjcC, which codes for a phosphodiesterase affecting expression.
The cold acclimatization response in many bacterial species is a tightly regulated process, which ensures the correct folding of macromolecules. In enterobacteria, this part dependent on polynucleotide phosphorylase, encoded by gene pnp. Based transcriptional analysis pnp locus Salmonella enterica serovar Typhimurium, we show that and adjacent membrane lipoprotein nlpI form an operon with both genes contributing independently to at 15 °C. Our findings thereby define new role for NlpI acclimatization.