A5056846880- Microbial Metabolic Engineering and Bioproduction
- Microbial metabolism and enzyme function
- Polysaccharides and Plant Cell Walls
- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Fungal Biology and Applications
- Microbial Natural Products and Biosynthesis
- Fungal and yeast genetics research
- Enzyme Catalysis and Immobilization
- Mycorrhizal Fungi and Plant Interactions
- Antibiotic Resistance in Bacteria
- Plant biochemistry and biosynthesis
- Gout, Hyperuricemia, Uric Acid
- Escherichia coli research studies
- biodegradable polymer synthesis and properties
- Alcohol Consumption and Health Effects
- Bacterial biofilms and quorum sensing
- GABA and Rice Research
- Plant pathogens and resistance mechanisms
- Higher Education and Teaching Methods
- Cancer, Hypoxia, and Metabolism
- Microbial Metabolites in Food Biotechnology
- Vibrio bacteria research studies
- Plant responses to elevated CO2
- Breastfeeding Practices and Influences
Jiangnan University
2009-2025
State Key Laboratory of Food Science and Technology
2022-2025
Changchun University of Science and Technology
2025
Jiangsu Second Normal University
2022-2025
Jiangsu University
2021-2024
Karolinska Institutet
2016-2023
Beijing Academy of Agricultural and Forestry Sciences
2022
Guangzhou University of Chinese Medicine
2022
Institute of Animal Science
2021
North Minzu University
2021
Cellulose, a 1,4 beta-glucan polysaccharide, is produced by variety of organisms including bacteria. Although the production cellulose has high biological, ecological and economical impact, regulatory mechanisms biosynthesis are mostly unknown. Family eight cellulases regularly associated with operons in bacteria; however, their function poorly characterized. In this study, we analysed role cellulase BcsZ encoded bcsABZC operon Salmonella enterica serovar Typhimurium (S. Typhimurium) biofilm...
Two-component systems (TCS) consisting of histidine kinases (HK) and response regulators (RR) play essential roles in bacteria to sense environmental signals regulate cell functions. One type RR is involved metabolism cyclic diguanylate (c-di-GMP), a ubiquitous bacterial second messenger. Although genomic studies predicted large number them existing different bacteria, only few have been studied. In this work, we characterized novel TCS PdeK(PXO_01018)/PdeR(PXO_ 01019) from Xanthomonas...
In the medical field, it is extremely important to use deep learning technology automatically generate diagnostic reports for chest X-ray images. This provides an effective solution challenges faced by field in processing large numbers of Especially during large-scale outbreaks epidemics such as new COVID-19, rapid and accurate screening diagnosis cases become tasks. study uses images, which significantly reduces workload doctors, risk misdiagnosis missed diagnosis, technical support...
In this present work, the polysaccharides from Agaricus bisporus were extracted and fractioned with gradient ethanol precipitation method for first time. Five fractions (ABP40, ABP50, ABP60, ABP70, ABP80) obtained concentrations of 40%, 50%, 60%, 70% 80%, respectively, their characteristics antioxidant activities in vitro investigated. The five presented significant differences total sugar, protein, uronic acid content, a marked discrepancy molar ration monosaccharide composition. molecular...
Bacteriophages have the destructive damage on industrial bioprocess. 2-Keto-gluconic acid (2KGA) producing bacteria had also been attacked and lysed by bacteriophages which lowered glucose consumption 2KGA yield even stopped fermentation process. In this study, we presented characteristics of a novel virulent bacteriophage specifically infecting Pseudomonas fluorescens K1005 proposed an efficient remedial action for phage infection to reduce production loss. The KSL-1 was isolated from...
UDP-glycosyltransferases (UGTs) are the most studied glycosyltransferases, and belong to large GT1 family performing key roles in antibiotic synthesis, development of bacterial glycosyltransferase inhibitors, animal inflammation. They transfer glycosyl groups from nucleotide UDP-sugars (UDP-glucose, UDP-galactose, UDP-xylose, UDP-rhamnose) acceptors including saccharides, proteins, lipids, secondary metabolites. The present review summarized recent UDP-glycosyltransferases, their structures,...
Oleaginous microorganisms have a large potential to be cell factory produce naringenin due their active fatty acid anabolism offering amounts of malonyl-CoA as the substrate for synthesis. However, only few studies reported production flavonoids in oleaginous microorganisms, e.g., Yarrowia lipolytica and Rhodosporidium toruloides. The fungus Mucor circinelloides produces β-carotene 15–36% dry weight lipids. This study is first time engineer an fungus, M. circinelloides, by heterologously...
Abstract Pathogenic bacteria use specific host factors to modulate virulence and stress responses during infection. We found previously that the factor bile component glyco-conjugated cholate (NaGCH, sodium glycocholate) upregulate colonization CS5 in enterotoxigenic Escherichia coli (ETEC). To further understand global regulatory effects of NaGCH, we performed Illumina RNA-Seq crude NaGCH altered expression 61 genes + CS6 ETEC isolates. The most striking finding was high induction operon (...
β-1,3-Glucan synthases play key roles in glucan synthesis, cell wall assembly, and growth of fungi. However, their multi-transmembrane domains (over 14 TMHs) large molecular masses 100 kDa) significantly hamper understanding catalytic characteristics mechanisms. In the present study, 5841-bp gene CMGLS encoding 221.7 kDa membrane-bound β-1,3-glucan synthase Cordyceps militaris was cloned, identified, structurally analyzed. partially purified with a specific activity 87.72 pmol/min/μg,...
A endo-1,4-β-mannanase (CcMan5C) gene was cloned from Coprinopsis cinerea and heterologously expressed in Pichia pastoris, the recombinant enzyme purified by Ni-affinity chromatography identified matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/TOF-MS). CcMan5C hydrolyzed only locust bean gum galactomannan (LBG) but not α-mannan S. cerevisiae or Avicel cellulose, oat spelt xylan, laminarin Laminaria digitata. exhibited distinctive catalytic features...
Grifola frondosa is a Basidiomycete fungus belonging to the family of Grifolaceae and order Polyporales. β-Glucans are main polymers in G. frondosa, playing crucial role physiology representing healthy benefits for humans. The membrane-integrated β-1, 3-glucan synthase (GLS) responsible glucan synthesis, cell wall assembly, differentiation growth edible fungi. However, structural/catalytic characteristics mechanisms synthases still unknown due their extremely complex structures with...
The bacterial pathogens enterotoxigenic