A5086837341- Cellular Mechanics and Interactions
- Force Microscopy Techniques and Applications
- Elasticity and Material Modeling
- Aortic aneurysm repair treatments
- Aortic Disease and Treatment Approaches
- Cardiomyopathy and Myosin Studies
- Cell Adhesion Molecules Research
- Mechanical and Optical Resonators
- Connective tissue disorders research
- Advanced Surface Polishing Techniques
- Cell Image Analysis Techniques
- Polymer Surface Interaction Studies
- Nanotechnology research and applications
- Wnt/β-catenin signaling in development and cancer
- Neuroscience and Neural Engineering
- Hippo pathway signaling and YAP/TAZ
- Air Quality and Health Impacts
- Noise Effects and Management
- Orthopaedic implants and arthroplasty
- PI3K/AKT/mTOR signaling in cancer
- Smoking Behavior and Cessation
Inserm
2019-2024
Mines Saint-Étienne
2019-2024
Université Jean Monnet
2019-2024
Université Claude Bernard Lyon 1
2017-2022
Biologie, ingénierie et imagerie pour l'Ophtalmologie
2019-2021
Centre Interuniversitaire de MicroElectronique et Nanotechnologies
2019
Université Grenoble Alpes
2019
Centre National de la Recherche Scientifique
2017
Laboratoire de Tribologie et Dynamique des Systèmes
2017
École Nationale d'Ingénieurs de Saint-Étienne
2017
Objective The role of YAP/TAZ, two transcriptional co-activators involved in several cancers, was investigated rheumatoid arthritis (RA). Methods Fibroblast like synoviocytes (FLS) from patients with RA or osteoarthritis were cultured 2D into 3D synovial organoids. Arthritis rat model (n=28) and colitis mouse (n=21) used. YAP/TAZ activity inhibited by verteporfin (VP). Multiple techniques used to assess gene and/or protein expression localization, cell phenotype (invasion, proliferation,...
Aortic smooth muscle cells (SMCs) play a vital role in maintaining mechanical homeostasis the aorta. We recently found that SMCs of aneurysmal aortas apply larger traction forces than healthy aortas. This result was explained by significant increase hypertrophic abundance aneurysms. In this study, we investigate whether cytoskeleton stiffness may also be altered For that, use atomic force microscopy (AFM) nano-indentation with specific mode allows subcellular-resolution mapping local across...
Smooth muscle cells (SMCs) usually express a contractile phenotype in the healthy aorta. However, aortic SMCs have ability to undergo profound changes response their extracellular environment, as occurs ascending thoracic aneurysms (ATAA). Accordingly, there is pressing need quantify mechanobiological effects of these at single cell level. To address this need, we applied Traction Force Microscopy (TFM) on 759 coming from three primary (AoPrim) human SMC lineages and aneurysmal (AnevPrim)...
The contractile behavior of smooth muscle cells (SMCs) in the aorta is an important determinant growth, remodeling, and homeostasis. However, quantitative values SMC basal tone have never been characterized precisely on individual SMCs. Therefore, to address this lack, we developed vitro technique based Traction Force Microscopy (TFM). Aortic SMCs from a human lineage at low passages (4-7) were cultured 2 days conditions promoting development their apparatus seeded hydrogels varying elastic...
The potential cardiovascular effects of e-cigarettes remain largely unidentified and poorly understood. E-liquids contain numerous chemical compounds can induce exposure to potentially toxic ingredients (e.g., nicotine, flavorings, etc.). Moreover, the heating process also lead formation new thermal decomposition that may be hazardous. Clinical as well in vitro vivo studies on e-cigarette toxicity have reported damages; however, results conflicting. aim this study was assess, vitro,...
Aortic smooth muscle cells (SMCs) play a vital role in maintaining homeostasis the aorta by sensing and responding to mechanical stimuli. However, mechanisms that underlie ability of SMCs sense respond stiffness change their environment are still partially unclear. In this study, we focus on acto-myosin contractility introduce novel continuum mechanics approach based principles thermal strains. Each stress fiber satisfies universal stress-strain relationship driven Young's modulus,...
Currently, more and organizations are aware of the increasing potential nanoscience nanotechnology, due to wide field innovative applications they address from biomedical molecular technology semiconductor material sciences. Nanotechnology is an area where fundamental or applicative research still in progress many industrial waited come. Despite this field, institutions, notably educational ones, cannot afford investing these technologies allowing access nanoscale, a lack knowledge therefore...
Science education is often limited by the complexity of making acute knowledge accessible and easily remembered a larger public. We proposed another way to introduce teach complex scientific subjects like cell biology polymer micro-rheology using haptic display virtual reality. combined advantages Atomic Force Microscopy (AFM) Fluorescence (FM) in order get complementary real experimental data on an isolated animal adhering protein micro-pattern. obtained high resolution mapping images...
Abstract Aortic Smooth Muscle Cells (SMCs) play a vital role in maintaining homeostasis the aorta by sensing and responding to mechanical stimuli. However, mechanisms that underlie ability of SMCs sense respond stiffness change their environment are still partially unclear. In this study, we focus on acto-myosin contractility introduce novel continuum mechanics approach based principles thermal strains. Each stress fiber satisfies universal stress-strain relationship driven Young’s modulus,...
La caractérisation des propriétés mécaniques de micro et nano-systèmes devient cruciale pour le développement (bio)MEMS/NEMS. Comme la microscopie à force atomique (AFM) est un instrument choix mesurer les morphologiques l’échelle submicronique, nous avons développé TP AFM en mode Peak Force sur cette thématique. Les étudiants effectueront cartographie 4D d’un échantillon polymères dont sont bien différentes. À partir ces cartographies AFM, estimeront modules Young sélectionnant le(s)...
Keywords: aortic smooth muscle cells (AoSMC)atomic force microscopy (AFM)cell biomechanicsisolated cellsnanomechanical mapping
Smooth Muscle Cells (SMCs) play a vital role in maintaining homeostasis the aorta by sensing and responding to mechanical stimuli. However, mechanisms that underlie ability of SMCs sense respond stiffness change their environment are still partially unclear. In this study, we focus on acto-myosin contractility introduce novel continuum mechanics approach based principles thermal strains. Each stress fiber satisfies universal stress-strain relationship driven Young’s modulus, contraction...