A5100622423- Planarian Biology and Electrostimulation
- Neuroscience and Neural Engineering
- Plant and Biological Electrophysiology Studies
- Photoreceptor and optogenetics research
- Ion channel regulation and function
- 3D Printing in Biomedical Research
- Pluripotent Stem Cells Research
- Geomechanics and Mining Engineering
- Retinal Development and Disorders
- Corneal Surgery and Treatments
- Marine Invertebrate Physiology and Ecology
- Wound Healing and Treatments
- Plant Disease Resistance and Genetics
- Genetic factors in colorectal cancer
- Neuroendocrine Tumor Research Advances
- Redox biology and oxidative stress
- Genomic variations and chromosomal abnormalities
- Evaluation Methods in Various Fields
- Ocular Diseases and Behçet’s Syndrome
- Connexins and lens biology
- Ginseng Biological Effects and Applications
- Advanced Nanomaterials in Catalysis
- Freezing and Crystallization Processes
- Cancer Research and Treatments
- Food Industry and Aquatic Biology
Kunming Medical University
2024
University of Aberdeen
2009-2023
Jiangxi Normal University
2023
Changhai Hospital
2022
Second Military Medical University
2022
Yunnan Agricultural University
2022
Sichuan University
2021
West China Hospital of Sichuan University
2021
Nanjing Forestry University
2021
Institute of Medical Sciences
2013-2015
Disrupted-in-Schizophrenia-1 ( DISC1 ), identified by positional cloning of a balanced translocation (1;11) with the breakpoint in intron 8 large Scottish pedigree, is associated range neuropsychiatric disorders including schizophrenia. To model this mutation mice, we have generated Disc1 tr transgenic mice expressing 2 copies truncated encoding first exons using bacterial artificial chromosome (BAC). With partial simulation human situation, discovered phenotypes series novel features not...
Directed cell migration is essential for tissue formation, inflammation, and wound healing. Chemotaxis plays a major role in these situations underpinned by asymmetric intracellular signaling. Endogenous electric fields (EFs) are common where movement occurs, such as healing, cells respond to field gradients reorienting migrating directionally (galvanotaxis/electrotaxis). We show that physiological EF redistributed both EGF (epidermal growth factor) receptors detergent-insoluble membrane...
The mechanisms by which cancer cells migrate to metastasise are not fully understood. Breast cancers accompanied electrical depolarisation of tumour epithelial cells. changes can be detected on the skin and used differentiate malignant from benign breast tumours. Could signals play a role in metastasis promoting cell migration? We report that electric fields stimulate direct migration human Importantly, these effects were more significant highly metastatic than low Electric-field-enhanced...
Breaking the balance between proliferation and differentiation in animal cells can lead to cancer, but mechanisms maintaining this remain largely undefined. The calcium activated chloride channel A1 (CLCA1) is a member of sensitive conductance family proteins expressed mainly colon, small intestine appendix. We show that CLCA1 plays functional role Caco-2 intestinal tissue. spontaneously differentiate either confluent culture or when treated with butyrate, molecule present naturally diet....
Although many pathogenic copy number variations (CNVs) are associated with neuropsychiatric diseases, few of them have been functionally characterised. Here we report multiple schizophrenia cases CNV abnormalities specific to unc-51-like kinase 4 (ULK4), a novel serine/threonine gene. Deletions spanning exons 21–34 ULK4 were present in out 3,391 patients from the International Schizophrenia Consortium, but absent 3,181 controls. removing 33 and 34 large splice variant also enriched Icelandic...
Chloride channel accessory 1 (CLCA1) is a CLCA protein that plays functional role in regulating the differentiation and proliferation of colorectal cancer (CRC) cells. Here we investigated relationship between level CLCA1 prognosis CRC.First, was detected quantitatively normal cancerous colonic epithelial tissues with immunohistochemistry. Next, correlations expression, pathological tumor features, overall survival rate patients analyzed. Finally, 3 publicly available data sets from Gene...
Observing cells in their original niche is a key link between the information gleaned from planar culture and vivo physiology pathology. A new approach combining transparency of cornea, Hoffman modulation optics, digital imaging allowed movements individual corneal to be viewed directly situ. 3-Dimensional time-lapse movies unstained within stratified epithelium during wound healing were made. Tracking cell dynamically provided definitive answer long-standing question: does heal by "sliding"...
Directional cell migration requires proper polarization. The redistribution of the Golgi apparatus is an important event in polarization and many types cells, as a polarized supplies membrane components for leading edge protrusion. Direct current electric fields induce directional wide variety cells. Here we show that 300 mV/mm robust CHO Asymmetric Src PI 3-kinase signalling well actin polymerization are essential field-induced migration. polarizes at same time cells change morphology...
Purpose.: The PI3K/Akt pathway is required for cell polarization and migration, whereas the phosphatase tensin homologue deleted on chromosome 10 (PTEN) has inhibitory effects pathway. authors therefore hypothesized that wounding would downregulate PTEN this downregulation enhance wound healing. Methods.: In human corneal epithelial (HCE) monolayer rat cornea scratch models, investigated Akt expression using Western blot immunofluorescence analyses. of PI3K inhibitors dipotassium bisperoxo...
Abstract Wound healing is a complex and well‐orchestrated biological process. Corneal epithelial cells (CECs) must respond quickly to trauma rapidly restore barrier function protect the eye from noxious agents. They express high level of ß2‐adrenergic receptors but their unknown. Here, we report novel finding that they form part regulatory network in corneal epithelium, capable modulating wound repair. ß‐adrenergic receptor agonists delay CEC migration via protein phosphatase 2A‐mediated...
Endogenous electrical fields (EFs) at corneal and skin wounds send a powerful signal that directs cell migration during wound healing. This therefore may serve as fundamental regulator directing polarization migration. Very little is known of the intracellular molecular mechanisms mediate EF-induced Here, we report Chinese hamster ovary (CHO) cells show robust directional in physiological EF (0.3–1 V/cm) both dissociated culture monolayer culture. An 0.6 V/cm completely abolished into higher...
Neural induction is largely considered a default process, whereas little known about intrinsic factors that drive neural differentiation. Kamiya and colleagues now demonstrate transcription factor, Zfp521, capable of directing embryonic stem (ES) cells into progenitors. They discovered Zfp521 transcripts were enriched in early lineage ES cell Forced expression turned progenitors culture conditions would normally inhibit was expressed mouse embryos during gastrulation. The protein shown to...
Neuroblasts migrate as directed chains of cells during development and following brain damage. A fuller understanding the mechanisms driving this will help define its developmental significance in refinement strategies for repair using transplanted stem cells. Recently, we reported that adult mouse there are ionic gradients within extracellular spaces create an electrical field (EF) rostral migratory stream (RMS), acts a guidance cue neuroblast migration. Here, demonstrate endogenous EF...
This study investigated the production of short-chain organic acids (SCOAs) and ethanol using anaerobic fermentation (AF) in semi-continuous CSTRs (continuously stirred tank reactors) with intermittent feed without solids separation SBRs (sequencing batch separation. A model feedstock, which included main classes substances present food waste, was used (24.7-394.6 gCOD L-1). To improve process sustainability, conditions uncontrolled pH room temperature were used. The effect concentration,...
The apicobasal polarity of enterocytes determines where the brush border membrane (apical membrane) will form, but how this apical faces lumen is not well understood. electrical signal across epithelium could serve as a coordinating cue, orienting and polarizing enterocytes. Here we show that applying physiological electric field (EF) to intestinal epithelial cells, mimic natural EF created by transepithelial potential difference, directed phosphorylation actin-binding protein ezrin,...