A5100321722- Thyroid Disorders and Treatments
- Vitamin D Research Studies
- Thyroid Cancer Diagnosis and Treatment
- Growth Hormone and Insulin-like Growth Factors
- Vitamin C and Antioxidants Research
- Dietary Effects on Health
- Adrenal Hormones and Disorders
- Clinical Laboratory Practices and Quality Control
- Alcohol Consumption and Health Effects
- Pituitary Gland Disorders and Treatments
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Hypothalamic control of reproductive hormones
- Biochemical and Molecular Research
- Meta-analysis and systematic reviews
- Metabolomics and Mass Spectrometry Studies
- Biosimilars and Bioanalytical Methods
- Adipokines, Inflammation, and Metabolic Diseases
- Bone health and osteoporosis research
- Mass Spectrometry Techniques and Applications
- Adipose Tissue and Metabolism
- Glioma Diagnosis and Treatment
- Protein Kinase Regulation and GTPase Signaling
- Pharmacology and Obesity Treatment
- Bone health and treatments
- Tuberculosis Research and Epidemiology
Chinese Academy of Medical Sciences & Peking Union Medical College
2017-2025
Peking Union Medical College Hospital
2017-2025
Second Affiliated Hospital of Nanjing Medical University
2024
Nanjing University
2023
Tsinghua University
2011
ABSTRACT Adequate vitamin K is crucial for optimal health. Although detection methods have been established using liquid chromatography–tandem mass spectrometry (LC–MS/MS), some limitations remain. Therefore, we aimed to establish a stable and rapid LC–MS/MS method that can quantify phylloquinone (VK1), menaquinones‐4 (MK‐4), menaquinones‐7 (MK‐7) in serum cerebrospinal fluid explore its clinical applications. We developed an with atmospheric pressure chemical ionization validate performance...
Yutong Zou, B.S., Danchen Wang, M.S., Xinqi Cheng, Chaochao Ma, Songbai Lin, Ph.D., Yingying Hu, Songlin Yu, Liangyu Xia, Honglei Li, Yicong Yin, Huaicheng Liu, Dianxi Zhang, Kui Xiaolan Lian, Tengda Xu, and Ling Qiu, M.S.. Ann Lab Med 2021;41:77-85. https://doi.org/10.3343/alm.2021.41.1.77
BackgroundThyroid hormone levels are essential for diagnosing and monitoring thyroid diseases. However, their reference intervals (RIs) in elderly Chinese individuals remain unclear. We aimed to identify factors affecting thyroid-stimulating (TSH), free triiodothyronine (FT3), thyroxine (FT4) using clinical "big data" establish level RIs individuals.
There are at least 250 enzymes in Mycobacterium tuberculosis (M. tuberculosis) involved lipid metabolism. Some of the required for bacterial survival and full virulence. The esterase Rv0045c shares little amino acid sequence similarity with other members esterase/lipase family. Here, we report 3D structure Rv0045c. Our studies demonstrated that is a novel member α/β hydrolase fold contains two distinct domains: domain cap domain. active site highly conserved comprised residues: Ser154...
Objectives Diagnosis of thyroid disease among individuals dwelling at high altitude remains a challenge. Reference intervals (RIs) for thyroid-associated hormones Tibetans living various altitudes were established to improve diagnosis. Methods One thousand two hundred eighty-one subjects randomly recruited from Nyingchi, Shigatse/Lhasa, and Ali Tibet. Thyroid-stimulating hormone (TSH), free triiodothyronine (FT3), thyroxine (FT4) measured by the Cobas e601 electrochemiluminescence analyzer....
Background Iodine, an essential nutrient, is the most important trace element in thyroid hormone synthesis and maintenance of function. This study investigated iodine nutrition status healthy Chinese adults assessed relationship between urinary concentration (UIC) levels. Methods A cross‐sectional, multicenter was conducted October 2017 January 2018, with 1017 recruited from five cities China. All subjects underwent ultrasonography, only those normal results were included study. UICs...
Summary Background Indirect sampling methods are not only inexpensive but also efficient for establishing reference intervals (RIs) using clinical data. This study was conducted to select fully normal records establish age- and gender-specific RIs common biochemical analytes by laboratory data mining. Methods In total, 280,206 from 2014 2018 were obtained Peking Union Medical College Hospital. Common [total protein, albumin, total bilirubin (TBil), direct (DBil), alanine aminotransferase...
Abstract Background The ALDH2 rs671 genetic polymorphism has been linked with cardiovascular diseases (CVDs), but comprehensive epidemiological studies are lacking. An observational, retrospective big data study was carried out to evaluate the associations between this and clustering risk factors (CRFs) in a Chinese population. Methods A total of 13,101 individuals (8431 males 4670 females) were enrolled. Genetic assessed using gene mutation detection kits, coupled an automatic fluorescent...
Accurate serum total thyroxine (TT4) measurement is important for thyroid disorder diagnosis and management. We compared the performance of six automated immunoassays with that isotope-diluted liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS) as reference method. also evaluated correlation stimulating hormone (TSH) TT4 measured by ID-LC-MS/MS immunoassays.Serum was collected from 156 patients between October 2015 January 2016. Abbott (Architect), Siemens (ADVIA Centaur XP), Roche...
Despite the need for precise measurement of thyroid hormones levels disease detection and monitoring, importance sampling time accuracy widely used assays is unclear. In this study, effects day on thyroid-stimulating hormone (TSH), free triiodothyronine (FT3), thyroxine (FT4) were retrospectively evaluated using data obtained from Laboratory Information System Peking Union Medical College Hospital. TSH concentrations significantly lower, FT4 FT3 higher in males than females (all p < .05)....
Abstract Background Serum lipid concentrations are affected by long-term high-fat diets; thus, we hypothesize that levels increase after the Spring Festival in China. Method In total, 20,192 individuals (male: n=10,108, female: n=10,084) were enrolled this retrospective cross-sectional study based on clinical data from Laboratory Information System (LIS) and Hospital (HIS) Peking Union Medical College 2014 to 2018. Total cholesterol (TC), triglycerides (TGs), high-density lipoprotein...
Survivin is an inhibitor of apoptosis. Its role in guiding the treatment neoplasms, making diagnosis and predicting prognosis has been reported. However, there little information on implications uses survivin pituitary adenoma (PA) invasiveness. Existing unclear controversial. We thus conducted this meta-analysis to explore whether surviving expression levels invasive PAs (IPA) regular are different or not. considered both non-secreting secreting tumors together.A global search strategy was...
Background Lp‐ PLA 2 is a novel inflammation marker in cardiovascular disease. While several manufactures have registered activity reagents, few studies investigated the consistency among these assays. In this study, we compared and recalibrated Methods Serum samples from 110 patients 140 healthy individuals were collected for method comparison reference interval validation, respectively. Fresh human serum pools (847 442 U/L) used recalibration. was analyzed using all five assays with...
This study aimed to establish a robust, simple method detect 25-hydroxyvitamin D3 (25(OH)D3 ), D2 (25(OH)D2 1,25-dihydroxyvitamin (1,25(OH)2 24,25-dihydroxyvitamin (24,25(OH)2 and ) simultaneously with efficient separation of 3-epi 25(OH)D3 , 24,25(OH)2 23R,25(OH)2 4β,25-dihydroxyvitamin (4β,25(OH)2 by liquid chromatography-tandem mass spectrometry (LC-MS/MS).This was validated according procedures established Clinical Laboratory Standards Institute (CLSI) then applied in healthy population...
To measure and evaluate the distribution possible contributing factors of seven bone metabolism-associated biomarkers in Tibet, a plateau province China.A total 1615 individuals were recruited from Tibet at three different altitudes. The levels serum calcium, phosphorus, ALP, 25OHD, PINP, CTX, PTH evaluated.In total, 1246 Tibetan adults (males: n = 543) eventually enrolled this study. Multiple linear regression recognized age, sex, altitude, BMI as major effect factors. CTX males...
Abstract Objective In our laboratory, 2.36% (6626/280765) samples obtained for insulin evaluation have serum concentrations higher than 300 mU/L, resulting in curves outside the linear range release test (IRT). Accordingly, using appropriate dilution protocols to determine concentration accurately is important. Here, we compared effectiveness and economy of four different solutions diluting high‐insulin routine clinical measurements. Method Residual with ranging from 200 mU/L were collected...