A5003851740- Adipose Tissue and Metabolism
- Cancer, Hypoxia, and Metabolism
- Angiogenesis and VEGF in Cancer
- Retinoids in leukemia and cellular processes
- Ion channel regulation and function
- Adipokines, Inflammation, and Metabolic Diseases
- Ion Transport and Channel Regulation
- Drug Transport and Resistance Mechanisms
- Mesenchymal stem cell research
- Nitric Oxide and Endothelin Effects
- Receptor Mechanisms and Signaling
- COVID-19 Clinical Research Studies
- Eicosanoids and Hypertension Pharmacology
- Cancer Cells and Metastasis
- Adenosine and Purinergic Signaling
- Heme Oxygenase-1 and Carbon Monoxide
- HIV Research and Treatment
- Neuroscience of respiration and sleep
- Neuropeptides and Animal Physiology
- Zebrafish Biomedical Research Applications
- Neuroscience and Neuropharmacology Research
- Glioma Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Restless Legs Syndrome Research
Inserm
1988-2022
Centre National de la Recherche Scientifique
2004-2022
Université Côte d'Azur
2011-2022
Institut de Biologie Valrose
2013-2022
Centre de Biologie du Développement
2005
Harvard University
2004
Beth Israel Deaconess Medical Center
2004
Brigham and Women's Hospital
2004
Jackson Laboratory
2004
Institut de Pharmacologie Moléculaire et Cellulaire
1991-2000
OBJECTIVE Growth of white adipose tissue takes place in normal development and obesity. A pool progenitors is responsible for the formation new adipocytes potential this to expand response chronic energy overload. However, factors controlling self-renewal human are largely unknown. We investigated expression profile role activin process. RESEARCH DESIGN AND METHODS Expression INHBA/activin was three types progenitors. then analyzed at molecular level function during adipogenesis. finally...
Histamine receptors are present on the surface of various normal and tumor-derived cell types, where their biological function is incompletely understood. Here we report that histamine not only stimulates proliferation under serum-free conditions, but also chemotactic for human carcinoma (Hela A431) melanoma (A875) cells expressing H1 type receptors. was found to be a potent activator phospholipase C, leading polyphosphoinositide hydrolysis subsequent intracellular Ca2+ mobilization. In...
Vascular endothelial growth factor (VEGF) is overexpressed in hyperproliferative diseases, such as psoriasis and cancers, which are characterized by increased angiogenesis. Experimentally, VEGF overexpression can be induced the treatment of cell cultures biological tissues with phorbol esters, 12-O-tetradecanoylphorbol-13-acetate (TPA). Using normal human keratinocytes conventional skin grafted onto nude mice vivo, we show that retinoids inhibit TPA-mediated gene induction at transcriptional...
Increasing attention has been paid to the effects of hypoxia, heavy metals and heat shocks on gene expression similarities in their actions. This paper compares mRNA levels two putative metal shock sensitive genes: heme oxygenase‐1 (HO‐1) vascular endothelial growth factor (VEGF) myocyte‐enriched cultures neonatal rat heart cells. HO‐1 is stimulated by hemin, Cd 2+ , Co but not Ni or Mn . It long (13 h) short (4 periods anoxia. Conversely, VEGF as well anoxia, hemin shocks. The results...
Abstract Human induced pluripotent stem cells (hiPSCs) show great promise for obesity treatment as they represent an unlimited source of brown/brite adipose progenitors (BAPs). However, hiPSC-BAPs display a low adipogenic capacity compared to adult-BAPs when maintained in traditional cocktail. The reasons this feature are unknown and hamper their use both cell-based therapy basic research. Here we that with TGFβ pathway inhibitor SB431542 together ascorbic acid EGF were required promote...
Endothelial cells from brain microvessels (BCEC) express high affinity receptor sites for endothelin-1 that recognize endothelin-3 with a low (Vigne, P., Marsault, R., Breittmayer, J.P. & Frelin, C. (1990) Biochem. J. 266, 415-420). Binding experiments using 125I-endothelin-3 showed the presence in BCEC of new class had (Kd = 0.8 nM), and sarafotoxin S6b 0.3 nM). Endothelins activated phospholipase C produced transient increases intracellular Ca2+ properties receptor. also increased 22Na+...
In cutaneous veins where purinergic neurotransmission is more prominent compared with in deep vessels, physiological and pathological roles of nerve-released ATP have been described. Neuronally released has reported to act through activation unidentified ionotropic P2X receptor(s). This study analyzed receptor subtypes expressed human saphenous vein smooth muscle their functions. Transcripts for both hP2X1 receptors, already identified other muscles, and, surprisingly, hP2X7 receptors known...
NCB5OR is a highly conserved NAD(P)H reductase that contains cytochrome b5-like domain at the N terminus and b5 reductase-like C terminus. The enzyme located in endoplasmic reticulum (ER) widely expressed organs tissues. Targeted inactivation of this gene mice has no impact on embryonic or fetal viability. At 4 weeks age, Ncb5or-/- have normal blood glucose levels but impaired tolerance. Isolated islets markedly glucose- arginine-stimulated insulin secretion. By 7 these develop severe...
Abstract Background Cancer cells cooperate with that compose their environment to promote tumor growth and invasion. Among them, adipocytes provide lipids used as a source of energy by cancer adipokines contribute expansion. Mechanisms supporting the dynamic interactions between stromal adipocytes, however, remain unclear. Methods We set-up co-culture model breast grown in 3D mammospheres human accurately recapitulate intrinsic features tumors, such hypoxia cell–adipocytes interactions....
Retinoic acid, which induces the differentiation of HL 60 cells to granulocytes, produces a cell alkalinization from pHi = 7.03 7.37. The half-maximum effect retinoic acid is observed at 10 nM. on develops slowly, and it precedes cells. A also after by dimethyl sulfoxide. It not using etretinate, synthetic retinoid that does promote Two regulating mechanisms coexist in Na+/H+ exchange system major mechanism allows recover an intracellular acidosis. second Na-HCO3 cotransport system. During...
The NAD(P)H cytochrome b5 oxidoreductase, Ncb5or (previously named b5+b5R), is widely expressed in human tissues and broadly distributed among the animal kingdom. NCB5OR first example of an flavohemoprotein containing chrome reductase cytodomains. We initially reported to be a 487-residue soluble protein that reduces c, methemoglobin, ferricyanide, molecular oxygen vitro. Bioinformatic analysis genomic sequences suggested presence upstream start codon. confirm endogenous indeed has...
U937 cell possess two mechanisms that allow them to recover from an intracellular acidification. The first mechanism is the amiloride‐sensitive Na + /H exchange system. second system involves bicarbonate ions. Its properties have been defined pH (pH i ) recovery experiments, 22 uptake 36 Cl − influx and efflux experiments. Bicarbonate induced of cells after a cellular acidification = 6.3 provided ions were present in assay medium. Li or K could not substitute for . seemed be electroneutral....