A5074958637- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Viral Infections and Vectors
- Vector-borne infectious diseases
- Leptospirosis research and findings
- Archaeological and Historical Studies
- Vector-Borne Animal Diseases
- Insect Pest Control Strategies
- Medieval Architecture and Archaeology
- Bartonella species infections research
- Parasitic Diseases Research and Treatment
- Parasites and Host Interactions
- Insect symbiosis and bacterial influences
- Genomics and Phylogenetic Studies
- Viral Infections and Outbreaks Research
- Insects and Parasite Interactions
- Plant and Fungal Interactions Research
- Ancient and Medieval Archaeology Studies
- Genetics, Bioinformatics, and Biomedical Research
- Intimate Partner and Family Violence
- Ancient Mediterranean Archaeology and History
- Mosquito-borne diseases and control
- Mollusks and Parasites Studies
Universidade Nova de Lisboa
2007-2023
Instituto de Investigação Científica Tropical
2015-2021
University of Lisbon
2015-2016
Zoonotic visceral leishmaniasis caused by Leishmania infantum which is transmitted phlebotomine sand flies (Diptera, Psychodidae) endemic in the Mediterranean basin. The main objectives of this study were to (i) detect DNA and (ii) identify blood meal sources wild caught female zoonotic region Algarve, Portugal/Southwestern Europe. Phlebotomine collected using CDC miniature light traps sticky papers. Sand identified morphologically tested for sp. PCR ITS-1 as target sequence. source engorged...
The emergence of drug-resistant strains Leishmania infantum, a parasite that infects dogs and humans, poses an increasing threat, underscoring the need for One Health framework adequate control. Aneuploidy gene copy-number variation (CNV) are associated with resistance to first-line drugs. We analyzed aneuploidies, genome plasticity, 15 CNVs as drug-resistance biomarkers in 35 L. infantum isolates from human canine host across Mediterranean by nanopore-only whole-genome sequencing automated...
The so-called canine vector-borne diseases (CVBD) are caused by a wide range of pathogens transmitted arthropods. In addition to their veterinary importance, many these can also affect the human population due zoonotic potential, situation that requires One Health approach. As prevalence in cats from southern Portugal has been recently evaluated, aim present study was assess if same agents were dogs living area, and positivity-associated risk factors. thousand ten (521 domestic 489 stray)...
Sandfly-borne phleboviruses are endemic in the Mediterranean basin. However, levels of exposure human and animal populations inadequately researched. Toscana virus (TOSV) is present Portugal where it causes infection disease; contrast there few data for sandfly fever Sicilian (SFSV) which has neither been isolated nor detected by molecular tests only limited serological data. The sera collected from 1160 dogs 189 cats southern were tested presence neutralizing antibodies against TOSV SFSV,...
Summary This study reports isoenzyme polymorphism of Leishmania strains isolated in different regions Portugal between 1982 and 2005. A total 213 were obtained from cases visceral cutaneous leishmaniasis immunocompetent patients (adults children) immunocompromised adults, as well dogs sandflies. Four zymodemes identified: MON‐1, MON‐24, MON‐29 MON‐80. Zymodeme MON‐1 was identified 96.7% the strains, predominating both human patients, it only zymodeme dogs. Isoenzyme diversity HIV‐infected...
The Algarve Region (AR) in southern Portugal, which is an international tourist destination, has been considered endemic region of zoonotic leishmaniasis caused by Leishmania infantum since the 1980s. In present study, phlebotomine and canine surveys were conducted to identify sandfly blood meal sources update occurrence infection vectors dogs. Four species captured: Phlebotomus perniciosus, ariasi, sergenti Sergentomyia minuta. one P. perniciosus female, L. DNA was detected. Blood tests...
Canine leishmaniosis (CanL) caused by Leishmania infantum is an important zoonosis in southwestern European countries where this disease endemic, and dogs, as domestic animals close contact with humans, are the reservoir hosts for parasite. In Portugal, CanL of relevant veterinary concern. The previous national study revealed overall seroprevalence 6.3%. Since then, new prophylactic measures, such vaccines, have been introduced Europe. aim was to update infection reassess risk factors...
Leishmaniosis caused by the protozoan Leishmania infantum and dirofilariosis nematodes Dirofilaria immitis or repens are vector-borne zoonoses widely present in Mediterranean basin. In addition, some studies reported that endosymbiont Wolbachia spp. play a role biology pathogenesis of filarial parasites. The aim this work was to evaluate frequency mono- co-infections L. infantum, filariae their association with clinical signs dogs from south Portugal. Leishmanial, DNA were evaluated specific...
Asymptomatic infection is the most common outcome of exposure to Leishmania parasites. In Mediterranean region, where infantum endemic, studies on prevalence asymptomatic have often relied serological testing in blood donors. Spain, regional shown seroprevalence donors between 1 and 8%; Portugal, values 0 2% were suggested by two localized studies, different populations. The purpose this study was (i) estimate mainland (ii) association detection antibodies sociodemographic factors, also...
SUMMARY Leishmania (Viannia) braziliensis is the main cause of highly disfiguring mucocutaneous leishmaniasis (MCL) in South America. The related species L. (V.) peruviana has only been identified simple cutaneous lesions (CL). Hybrids between and have reported although genetic exchange considered to be rare. Here we compared growth vitro, adaptive capacity under thermal oxidative stress behaviour a hamster model, braziliensis, , their putative hybrids. At 24°C, optimal temperature for vitro...
Leishmaniosis, dirofilariosis and angiostrongylosis are parasitic diseases of established importance in dogs worldwide. The aim the present report was to determine all together levels infection with or exposure Leishmania infantum, Dirofilaria immitis Angiostrongylus vasorum from Algarve region, southern Portugal. Serum samples were obtained a total 170 apparently healthy dogs. Antibodies L. infantum detected by direct agglutination test; antigens D. A. an enzyme-linked immunosorbent assay...
Abstract The frequency of sandfly–host contacts can be measured by host antibody levels against sandfly salivary proteins. Recombinant proteins are suggested to represent a valid replacement for gland homogenate ( SGH ); however, it is necessary prove that such antigens recognized antibodies various populations the same species. P hlebotomus perniciosus D iptera: sychodidae) main vector L eishmania infantum T rypanosomatida: rypanosomatidae) in southwest E urope and widespread from ortugal I...
This is the first clinical report of feline viscerocutaneous leishmaniosis caused by Leishmania infantum genotype E associated with an invasive squamous cell carcinoma (SCC) in a domestic cat from Portugal. Initially, presented single cutaneous lesion right nostril. A fine-needle aspiration was performed and amastigotes were observed without presence cells compatible neoplasia. Systemic treatment allopurinol started. One year later, crateriform non-encapsulated badly delineated mass nasal...
Phlebotomine sand fly-borne diseases such as leishmanioses and phleboviruses are emerging threats to animal public health. Canine leishmaniosis caused by Leishmania infantum is an endemic zoonosis in Portugal. Antibodies Toscana virus (TOSV) fly fever Sicilian (SFSV) were also reported dogs from the south of country. The aim this work was evaluate a possible association between exposure L. infantum, TOSV SFSV sheltered Seventy-six (13.1%) out 581 seropositive for 327 (56.3%) 36 (6.2%) TOSV....
Abstract Background In endemic areas of zoonotic leishmaniosis caused by L. infantum , early detection Leishmania infection in dogs is essential to control the dissemination parasite humans. The aim this study was evaluate serological and/or molecular diagnostic performance minimally and non-invasive samples (conjunctiva cells (CS) peripheral blood (PB)) for monitoring infection/exposure Phlebotomus perniciosus salivary antigens at beginning end sand fly seasonal activity (May October,...
Background Leishmaniasis, caused by Leishmania protozoan parasites transmitted Phlebotomine sand flies, is a significant public health concern in the Mediterranean basin. Effective monitoring of -infected flies requires standardized tools for comparing their distribution and infection prevalence. Consistent quantitative real-time PCR (qPCR) parameters efficient DNA extraction protocols are crucial reliable results over time across regions. However, absence technical recommendations detection...
Zoonotic leishmaniosis, caused by the protozoan Leishmania infantum, is a public and animal health problem in Asia, Central South America, Middle East Mediterranean Basin. Several phlebotomine sand fly species from subgenus Larroussius are vectors of L. infantum. Data dogs living endemic areas leishmaniosis advocate use antibody response to saliva as an epidemiological biomarker for monitoring vector exposure. The aim this study was analyse exposure cats flies using detection IgG antibodies...