A5014768890- Congenital heart defects research
- Neural dynamics and brain function
- Congenital Heart Disease Studies
- Pluripotent Stem Cells Research
- Neuroscience and Neural Engineering
- Mitochondrial Function and Pathology
- EEG and Brain-Computer Interfaces
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- Neuroscience and Neuropharmacology Research
- Neuroscience and Music Perception
- Metabolism and Genetic Disorders
- RNA Interference and Gene Delivery
- Coronary Artery Anomalies
- Pancreatic function and diabetes
- Genomic variations and chromosomal abnormalities
- Memory and Neural Mechanisms
- Receptor Mechanisms and Signaling
- Pneumocystis jirovecii pneumonia detection and treatment
- Atrial Fibrillation Management and Outcomes
- Genetics and Neurodevelopmental Disorders
- Bacterial Infections and Vaccines
- Peptidase Inhibition and Analysis
- Advanced Battery Materials and Technologies
- Liver physiology and pathology
Qingdao University
2025
Affiliated Hospital of Qingdao University
2025
Vanderbilt University Medical Center
2025
University of Pittsburgh
2018-2024
Cincinnati Children's Hospital Medical Center
2024
University of California, San Diego
2023-2024
Chinese Academy of Sciences
2008-2023
Center for Excellence in Brain Science and Intelligence Technology
2020-2023
University of Chinese Academy of Sciences
2014-2020
Fiona Stanley Hospital
2018
The direct conversion, or transdifferentiation, of non-cardiac cells into cardiomyocytes by forced expression transcription factors and microRNAs provides promising approaches for cardiac regeneration. However, genetic manipulations raise safety concerns are thus not desirable in most clinical applications. discovery full chemically induced pluripotent stem suggest the possibility replacing with chemical cocktails. Here, we report generation automatically beating cardiomyocyte-like from...
Somatic cells can be reprogrammed into induced pluripotent stem (iPSCs) by defined factors. The low efficiency of reprogramming and genomic integration oncogenes viral vectors limited the potential application iPSCs. Here we report that Lithium (Li), a drug used to treat mood disorders, greatly enhances iPSC generation from both mouse embryonic fibroblast human umbilical vein endothelial cells. Li facilitates with one (Oct4) or two factors (OS OK). effect on promoting only partially depends...
Prelithiation plays a crucial role in advancing the development of high-energy-density batteries, and ultrathin lithium (UTL) has been proven to be promising anode prelithiation reagent. However, there remains need explore an adjustable, efficient, cost-effective method for manufacturing UTL. In this study, we introduce producing UTL with adjustable thicknesses ranging from 1.5 10 μm through blade coating molten on poly(vinylidene fluoride)-modified copper current collectors. By employing...
Primary hepatocytes cultured in vitro are a powerful tool to study the functions of and evaluate metabolism toxicity new drugs. However, culture has proven be very difficult. Ordinary conditions lead dedifferentiation hepatocytes, resulting rapid change cell morphology significant reduction specific functions. In current study, we show that hepatocyte is process under 21% O2 conditions. Hepatocytes undergo epithelial-to-mesenchymal transition (EMT), obtain fibroblast-like morphology,...
Our previous work identified a 12-amino acid peptide that targets the heart, termed cardiac targeting (CTP). We now quantitatively assess bio-distribution of CTP, show clinical application with imaging murine and study its mechanisms transduction. Bio-distribution studies cyanine5.5-N-Hydroxysuccinimide (Cy5.5) labeled CTP were undertaken in wild-type mice. Cardiac was Technetium 99m (99mTc) using chelator hydrazino-nicotinamide (HYNIC), performed micro-single photon emission computerized...
Maintaining the self-renewal of embryonic stem cells (ESCs) could be achieved by activating extrinsic signaling, i.e., use leukemia inhibitory factor (LIF), or blocking intrinsic differentiation pathways, GSK3 and MEK inhibitors (2i). Here we found that even in medium supplemented with LIF, mESCs still tend to differentiate toward meso-endoderm lineages after long-term culture spontaneously secretes vascular endothelial growth factors (VEGFs). Blocking VEGF signaling sunitinib, an...
Abstract Flexible electrodes have demonstrated better biocompatibility than rigid in relieving tissue encapsulation and long-term recording. Nonhuman primates are closer to humans their brains’ structural functional properties, thus making them more suitable rodents as animal models for potential clinical usage. However, the application of flexible on nonhuman has rarely been reported. In present study, a multichannel electrode array was developed implemented extracellular recording behaving...
Environmental stress-mediated adaptation plays essential roles in the evolution of life. Cellular mechanisms usually involve regulation chromatin structure, transcription, mRNA stability and translation, which eventually lead to efficient changes gene expression. Global epigenetic change is also involved reprogramming somatic cells into induced pluripotent stem (iPS) by defined factors. Here we report that environmental stress such as hyperosmosis not only facilitates four factor-mediated...
Responses to repeated auditory stimuli were examined in 103 neurons the region of thalamic reticular nucleus (TRN) and 20 medial geniculate (MGB) anesthetized rats. A further six TRN recorded from awake The showed strong responses first trial weak subsequent trials electrical stimulation MGB cortex when interstimulus interval (ISI) was short (<3 s). They responded second lengthened >or=3 s. These contrasted those neurons, which different ISIs. a significant increase onset response 9.5 76.5...
Abstract The recent recovery of mutations in vesicular trafficking genes causing congenital heart disease (CHD) revealed an unexpected role for the endocytic pathway. We now show that mice with a C4232R missense mutation Low density lipoprotein receptor related protein 1 (LRP1) exhibit atrioventricular septal defects double outlet right ventricle. Lrp1 m/m shortened outflow tracts (OFT) and dysmorphic hypocellular cushions reduced proliferation increased apoptosis. embryonic fibroblasts...
Background: Congenital heart disease (CHD) with single-ventricle (SV) physiology is now survivable a three-stage surgical course ending Fontan palliation. However, 10-year transplant-free survival remains at 39-50%, ventricular dysfunction progressing to failure (HF) being common sequela. For SV-CHD patients who develop HF, undergoing the would not be helpful and could even detrimental. As HF risk cannot predicted metabolic defects have been observed in Ohia mice, we hypothesized that...
Analysis of large-scale human genomic data has yielded unexplained mutations known to cause severe disease in healthy individuals. Here, we report the unexpected recovery a rare dominant lethal mutation TPM1, sarcomeric actin-binding protein, eight individuals with large atrial septal defect (ASD) five-generation pedigree. Mice Tpm1 exhibit early embryonic lethality disrupted myofibril assembly and no heartbeat. However, patient-induced pluripotent-stem-cell-derived cardiomyocytes show...
The ability to detect unexpected or deviant events in natural scenes is critical for survival. In the auditory system, neurons from midbrain cortex adapt quickly repeated stimuli but this adaptation does not fully generalize other, rare stimuli, a phenomenon called stimulus-specific (SSA). Most studies of SSA were conducted with pure tones different frequencies, and it by now well-established that tone frequency strong robust cortex. Here we tested ear stimulation using broadband noise. We...
Hypoplastic left heart syndrome (HLHS) is a relatively rare severe congenital defect (CHD) closely linked to other ventricular outflow tract (LVOT) lesions including bicuspid aortic valve (BAV), one of the most common defects. While HLHS, BAV, and LVOT have strong genetic underpinning, their etiology remains poorly understood. Findings from large-scale mouse mutagenesis screen showed HLHS has multigenic genetically heterogenous, explaining difficulties in identifying causes HLHS. In
Abstract Brain-machine interfaces (BMIs) are being developed for translating neural signals to control an external device such as a robotic arm. The prevalent decoding strategy of current BMIs is the continuously conversion spike trains motor variables via discriminative algorithm, but this typically results in un-natural and slow motion. Recently, we implemented BMI under feedforward interception moving targets, though accuracy was insufficient accurate online control. To improve...
Summary Hypoplastic left heart syndrome (HLHS) is a severe congenital defect with 30% mortality from failure (HF) in the first year of life, but why only some patients suffer early-HF and its cause remain unknown. Modeling using induced pluripotent stem cell-derived cardiomyocytes (iPSC-CM) showed patient iPSC-CM have increased apoptosis, redox stress, failed antioxidant response. This was associated mitochondrial permeability transition pore (mPTP) opening, hyperfusion respiration defects....