A5047283974- Influenza Virus Research Studies
- interferon and immune responses
- Respiratory viral infections research
- Immune Response and Inflammation
- Virology and Viral Diseases
- Vibrio bacteria research studies
- Trace Elements in Health
- Viral Infections and Vectors
- Complement system in diseases
- Heavy Metal Exposure and Toxicity
- Chromium effects and bioremediation
- Bacteriophages and microbial interactions
- Plant Virus Research Studies
- RNA modifications and cancer
Imperial College London
2017-2020
University of Leeds
2015
New vaccine platforms are needed to address the time gap between pathogen emergence and licensure. RNA-based vaccines an attractive candidate for this role: they safe, produced cell free, can be rapidly generated in response emergence. Two RNA available: synthetic mRNA molecules encoding only antigen of interest self-amplifying (sa-RNA). sa-RNA is virally derived encodes both proteins enabling replication. Both have been shown induce immune response, but it not clear which approach optimal....
Abstract Objectives To gain a more detailed understanding of endogenous (mutational) and exogenous (horizontally acquired) resistance to silver in Gram-negative pathogens, with an emphasis on clarifying the genetic bases for resistance. Methods A suite microbiological molecular techniques was employed select characterize several species. Results In Escherichia coli, arose after 6 days exposure silver, consequence two point mutations that were both necessary sufficient phenotype. These...
Cellular intrinsic immunity, mediated by the expression of an array interferon-stimulated antiviral genes, is a vital part host defense. We have previously used bioinformatic screen to identify two genes (ISG) with poorly characterized function, interferon-induced protein 44 (IFI44) and 44-like (IFI44L), as potentially being important in respiratory syncytial virus (RSV) infection. Using overexpression systems, CRISPR-Cas9-mediated knockout, knockout mouse model, we investigated capability...
Host susceptibility to viral infection is multifactorial, but early control of viruses not previously encountered predominantly mediated by the interferon-stimulated gene (ISG) family. There are upwards 300 these genes, majority which do have a clearly defined function or mechanism action. The cellular location proteins may an important effect on their function. One ISG located at plasma membrane interferon-inducible transmembrane protein 1 (IFITM1). Here we demonstrate that IFITM1 can...
Paramyxoviruses can establish persistent infections both in vitro and vivo , some of which lead to chronic disease. However, little is known about the molecular events that contribute establishment by RNA viruses. Using parainfluenza virus type 5 (PIV5) as a model we show phosphorylation P protein, key component viral polymerase complex, determines whether or not transcription replication becomes repressed at late times after infection. If repressed, persistence established, but if not,...
The initiation of an immune response is dependent on the activation and maturation dendritic cells after sensing pathogen associated molecular patterns by pattern recognition receptors. However, needs to be balanced as excessive pro-inflammatory cytokine production in viral or stress-induced receptor signaling has been with severe influenza A virus (IAV) infection. Here, we use inhibitor Toll-like (TLR)3, a single-stranded oligonucleotide (ssON) capacity inhibit certain endocytic routes,...
Current influenza vaccines manufactured using eggs have considerable limitations, both in terms of scale up production and the potential impact passaging through can on antigenicity vaccine virus strains. Alternative methods manufacture are required, particularly context an emerging pandemic strain. Here we explore recombinant haemagglutinin ciliated protozoan Tetrahymena thermophila. For first time were able to produce from seasonal A B This ciliate derived material was immunogenic,...
Abstract Cellular intrinsic immunity, mediated by the expression of an array interferon-stimulated antiviral genes, is a vital part host defence. We have previously used bioinformatic screen to identify two interferon stimulated genes (ISG) with poorly characterised function, Interferon-induced protein 44 (IFI44) and interferon-induced 44-like (IFI44L), as potentially being important in Respiratory Syncytial Virus (RSV) infection. Using overexpression systems, CRISPR-Cas9-mediated knockout,...