A5036336157- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Salmonella and Campylobacter epidemiology
- Viral gastroenteritis research and epidemiology
- COVID-19 Clinical Research Studies
- Vibrio bacteria research studies
- RNA Interference and Gene Delivery
- Immune Response and Inflammation
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Animal Virus Infections Studies
- Escherichia coli research studies
- CAR-T cell therapy research
- Bacterial Infections and Vaccines
- Pneumonia and Respiratory Infections
- Influenza Virus Research Studies
- Hepatitis B Virus Studies
- Pregnancy-related medical research
- Immune responses and vaccinations
- HIV/AIDS Research and Interventions
- Cardiac electrophysiology and arrhythmias
- Rheumatoid Arthritis Research and Therapies
- HIV/AIDS oral health manifestations
- Phagocytosis and Immune Regulation
University of Siena
2015-2025
Libera Università Maria SS. Assunta
2023-2024
Ospedale San Giuseppe
2024
University of Palermo
2023
SARS-CoV-2 mRNA vaccines have demonstrated high efficacy and immunogenicity, but limited information is currently available on memory B cell generation long-term persistence. Here, we investigated spike-specific cells humoral responses in 145 subjects, up to 6 months after the BNT162b2 vaccine (Comirnaty) administration. Spike-specific antibodies peaked 7 days second dose significant antibody titers ACE2/RBD binding inhibiting activity were still observed months, despite a progressive...
Adjuvants contribute to enhancing and shaping the vaccine immune response through different modes of action. Since primary can influence overall quality generated, here we investigate early biomarkers adjuvanticity after immunization with four adjuvants combined chimeric tuberculosis antigen H56. C57BL/6 mice were immunized by subcutaneous route formulations, modulation CD4+ T cell B responses was assessed within draining lymph nodes, blood spleen, 7 12 days priming. Vaccine formulations...
Limited longitudinal data are available on immune response to mRNA SARS-CoV-2 vaccination in people living with HIV (PLWHIV); therefore, new evidence induction and persistence of spike-specific antibodies B cells is needed.In this pilot study we investigated the humoral cell responses up six months after two doses vaccines 84 PLWHIV under antiretroviral therapy compared 79 healthy controls (HCs).Spike-specific IgG persisted no significant differences HCs, even though a significantly lower...
Combinations of different delivery routes for priming and boosting represent vaccination strategies that can modulate magnitude, quality, localization the immune response. A murine model was used to study T cell clonal expansion following intranasal (IN) or subcutaneous (SC) priming, secondary responses after by either homologous heterologous routes. primary activation studied using adoptive transfer ovalbumin-specific transgenic CD4(+) cells. Both IN SC immunization efficiently elicited, in...
Heterologous prime-boost schedules have been employed in SARS-CoV-2 vaccination, yet additional data on immunogenicity and effectiveness are still needed.
Vaccination against SARS-CoV-2 using mRNA-based vaccines has been highly recommended for fragile subjects, including myelofibrosis patients (MF). Available data on the immune responsiveness of MF to mRNA vaccination, and impact therapy with JAK inhibitor ruxolitinib, are still fragmented. Here, we profile spike-specific IgG memory B-cell response in patients, treated or not after second third dose BNT162b2 (BioNTech) mRNA-1273 (Moderna) vaccines. Plasma peripheral blood mononuclear cells...
Priming of T cells is a key event in vaccination, since it bears decisive influence on the type and magnitude immune response. T-cell priming after mucosal immunization via nasal route was studied by investigating distribution antigen-loaded antigen presenting (APCs) primed antigen-specific cells. Nasal studies were conducted using model protein ovalbumin (OVA) plus CpG oligodeoxynucleotide adjuvant. Trafficking analyzed vivo adoptive transfer OVA-specific transgenic presence or absence...
Transcriptomic profiling of the immune response induced by vaccine adjuvants is critical importance for rational design vaccination strategies. In present study, transcriptomics was employed to profile effect adjuvant used priming on following re-exposure antigen alone. mRNA sequencing performed blood samples collected (1, 2 and 7 days after boosting) from mice primed with chimeric H56 Mycobacterium tuberculosis administered alone or CAF01 boosted Gene expression analysis at day showed that...
Immunization with mRNA SARS-CoV-2 vaccines has been highly recommended and prioritized in fragile subjects, including patients myelofibrosis (MF). Available data on the vaccine immune response developed by MF impact of ruxolitinib treatment are still too fragmented to support an informed decision a third dose for this category subjects. Here, we show that 76% develop spike-specific IgG after second dose, but slower kinetics compared healthy suggesting reduced capability their system promptly...
Characterizing the impact of vaccination schedule on induction B and T cell immune responses is critical for improving vaccine immunogenicity. Here we compare effect a short (4 weeks) or long (18 interval between priming boosting in mice, using model formulation based chimeric tuberculosis antigen H56 combined with alum. While no significant difference was observed serum antigen-specific IgG response follicular helper cells into draining lymph nodes after two immunization schedules, longer...
The mRNA vaccines for SARS-CoV-2 have demonstrated efficacy and immunogenicity in the real-world setting. However, most of research on vaccine has been centered characterizing antibody response, with limited exploration into persistence spike-specific memory B cells. Here we monitored durability cell response up to 9 months post-vaccination, characterized trajectory phenotypes healthy individuals who received two doses BNT162b2 vaccine. To profile applied tSNE Cytotree automated approaches....
Preventing SARS-CoV-2 infection is of utmost importance in allogeneic hematopoietic cell transplantation patients (allo-HCT), given their heightened susceptibility to adverse outcomes associated with infection. However, limited data are available regarding the immune response COVID-19 vaccines these subjects, particularly concerning generation and persistence spike-specific memory response. Here, we analyzed B cells a cohort allo-HCT recipients vaccinated multiple doses mRNA-1273 vaccine...
Salmonella enterica serovars Typhimurium and Enteritidis are the predominant causes of invasive nontyphoidal (iNTS) disease. Considering co-endemicity Enteritidis, a bivalent vaccine formulation against both pathogens is necessary for protection iNTS disease, thus investigation glycoconjugate combination required. In present work, we investigated immune responses induced by S. monovalent vaccines adjuvanted with alum only or in CpG. Humoral cellular, local systemic, were characterized two...
Abstract Aim To investigate the bidirectional influence between periodontitis and psoriasis, using respective experimental models of ligature‐ imiquimod‐induced diseases on murine models. Materials Methods Thirty‐two C57/BL6J mice were randomly allocated to four groups: control (P− Pso−), ligature‐induced (P+ psoriasis Pso+) Pso+). Samples (maxilla, dorsal skin blood) harvested immediately after death. Measures (distance cemento‐enamel junction alveolar bone crest [CEJ–ABC] number...
The mRNA vaccines for SARS-CoV-2 have demonstrated efficacy and immunogenicity in the re-al-world setting. However, most of research on vaccine has been centered characterizing antibody response, with limited exploration into persistence spike-specific memory B cell response. Here we monitored durability response characterize trajectory phenotypes healthy individuals who received two doses BNT162b2 up to 9 months post-vaccination. To profile applied tSNE Cytotree automated approaches. Our...
Primary T-cell activation at mucosal sites is of utmost importance for the development vaccination strategies. priming after vaginal immunization, with ovalbumin and CpG oligodeoxynucleotide adjuvant as model vaccine formulation, was studied in vivo hormone-synchronized mice compared to one induced by nasal route. Twenty-four hours both or antigen-loaded dendritic cells were detected within respective draining lymph nodes. Vaginal immunization elicited a strong recruitment antigen-specific...
The induction and modulation of the immune response to vaccination can be rationally designed by combining different vaccine formulations for priming boosting. Here, we investigated impact heterologous prime-boost approaches on vaccine-specific cellular humoral responses specific a mycobacterial antigen. C57BL/6 mice were primed with chimeric antigen H56 administered alone or CAF01 adjuvant, boosted alone, combined squalene-based oil-in-water emulsion (o/w Squalene) adjuvant. A strong...
The generation of the B cell response upon vaccination is characterized by induction different functional and phenotypic subpopulations strongly dependent on vaccine formulation, including adjuvant used. Here, we have profiled subsets elicited vaccination, using machine learning methods for interpreting high‐dimensional flow cytometry data sets. an adjuvanted compared to antigen alone, was two automated based clustering (FlowSOM) dimensional reduction (t‐SNE) approaches. method identified,...
ABSTRACT SARS-CoV-2 mRNA vaccines have demonstrated high efficacy and immunogenicity, but limited information is currently available on memory B cells generation long-term persistence. Here, we investigated Spike-specific humoral responses in 145 subjects, up to six months after the BNT162b2 vaccine (Comirnaty) administration. antibody titers peaked 7 days second dose significant neutralizing activity were still observed months, despite a progressive decline over time. Concomitant reduction,...
Typhoid fever is a public health problem, especially among young children in developing countries. To address this need, glycoconjugate vaccine Vi-CRM₁₉₇, composed of the polysaccharide antigen Vi covalently conjugated to non-toxic mutant diphtheria toxin CRM₁₉₇, under development. Here, we assessed antibody and cellular responses, both local systemic, following subcutaneous injection Vi-CRM₁₉₇. The elicited Vi-specific serum IgG titers significantly higher than unconjugated Vi, with...