A5074564268- Computational Drug Discovery Methods
- Cancer-related Molecular Pathways
- Cholinesterase and Neurodegenerative Diseases
- Viral gastroenteritis research and epidemiology
- Escherichia coli research studies
- Chronic Lymphocytic Leukemia Research
- Acute Myeloid Leukemia Research
- Alzheimer's disease research and treatments
- Immune Response and Inflammation
- Lymphoma Diagnosis and Treatment
- Clostridium difficile and Clostridium perfringens research
- Analytical Chemistry and Chromatography
- Pneumonia and Respiratory Infections
- Bacterial Infections and Vaccines
- Ubiquitin and proteasome pathways
- Cancer, Hypoxia, and Metabolism
- Protein purification and stability
- Cancer Research and Treatments
- Chronic Myeloid Leukemia Treatments
- Monoclonal and Polyclonal Antibodies Research
- Antimicrobial Resistance in Staphylococcus
- Salmonella and Campylobacter epidemiology
- Immunotherapy and Immune Responses
- Immunodeficiency and Autoimmune Disorders
- Chemical Synthesis and Analysis
University of Bologna
2006-2025
Toscana Life Sciences
2015-2024
Sapienza University of Rome
2009-2023
Global Vaccines (United States)
2023
Agostino Gemelli University Polyclinic
2021
Istituti di Ricovero e Cura a Carattere Scientifico
2021
GlaxoSmithKline (Italy)
2021
Institute of Cell Biology and Neurobiology
2012-2019
Università Cattolica del Sacro Cuore
2005-2019
National Research Council
2007-2019
Platinum-based anticancer drugs cause neurotoxicity. In particular, oxaliplatin produces early-developing, painful, and cold-exacerbated paresthesias. However, the mechanism underlying these bothersome dose-limiting adverse effects is unknown. We hypothesized that transient receptor potential ankyrin 1 (TRPA1), a cation channel activated by oxidative stress cold temperature, contributes to mechanical hypersensitivity caused cisplatin. Oxaliplatin cisplatin evoked glutathione-sensitive...
A novel series of donepezil−tacrine hybrids designed to simultaneously interact with the active, peripheral and midgorge binding sites acetylcholinesterase (AChE) have been synthesized tested for their ability inhibit AChE, butyrylcholinesterase (BChE), AChE-induced Aβ aggregation. These compounds consist a unit tacrine or 6-chlorotacrine, which occupies same position as at AChE active site, 5,6-dimethoxy-2-[(4-piperidinyl)methyl]-1-indanone moiety donepezil (or indane derivative thereof),...
The outcome of children and adults with acute lymphoblastic leukemia is markedly different. Since there limited information on the distribution clinico-biological variables in different age cohorts, we analyzed 5202 patients enrolled Italian multicenter AIEOP GIMEMA protocols stratified them nine cohorts. highest prevalence was observed children, although a second peak recorded from 4th decade onwards. Interestingly, lowest incidence found females between 14–40 years. Immunophenotypic...
Molecular interactions between male and female factors during mating profoundly affect the reproductive behavior physiology of insects. In natural populations malaria mosquito Anopheles gambiae, blood-fed females direct nutritional resources towards oogenesis only when inseminated. Here we show that mating-dependent pathway egg development in these mosquitoes is regulated by interaction steroid hormone 20-hydroxy-ecdysone (20E) transferred males copulation a Mating-Induced Stimulator...
A family of huprine-tacrine heterodimers has been developed to simultaneously block the active and peripheral sites acetylcholinesterase (AChE). Their dual site binding for AChE, supported by kinetic molecular modeling studies, results in a highly potent inhibition catalytic activity human AChE and, more importantly, vitro neutralization pathological chaperoning effect toward aggregation both β-amyloid peptide (Aβ) prion with key role protein. Huprine-tacrine take on added value that they...
Abstract Induction of persistent protective immune responses is a key attribute successful vaccine formulation. MF59 adjuvant, an oil-in-water emulsion used in human vaccines, known to induce high-affinity functional Ab titers and memory B cells, but how it really shapes the Ag-specific cell compartment poorly documented. In this study, we characterized Ab- wild-type mice immunized with HlaH35L, Staphylococcus aureus Ag measurable responses, formulated or aluminum salts, focusing on germinal...
The biological diversity of marine habitats is a unique source chemical compounds with potential use as pharmaceuticals, cosmetics and dietary supplements. However, screening analysis extracts pose specific technical constraints require adequate sample preparation. Here we report an improved method on Solid Phase Extraction (SPE) to fractionate organic containing high concentration salt that hampers the recovery secondary metabolites. procedure uses water suspension load...
Technology platforms are an important strategy to facilitate the design, development and implementation of vaccines combat high-burden diseases that still a threat for human populations, especially in low- middle-income countries, address increasing number global distribution pathogens resistant antimicrobial drugs. Generalized Modules Membrane Antigens (GMMA), outer membrane vesicles derived from engineered Gram-negative bacteria, represent attractive technology design affordable vaccines....
We report data from Stage 1 of an ongoing two-staged, phase I/II randomized clinical trial (NCT05073003) with a 4-component Generalized Modules for Membrane Antigens-based vaccine against Shigella sonnei and S. flexneri 1b, 2a 3a (altSonflex1-2-3, GSK).
A rapidly acting, single dose vaccine against Staphylococcus aureus would be highly beneficial for patients scheduled major surgeries or in intensive care units. Here we show that one immunization with a multicomponent S. candidate vaccine, 4C-Staph, formulated novel TLR7-dependent adjuvant, T7-alum, readily protected mice from death and bacterial dissemination, both kidney abscess peritonitis models, outperforming alum-formulated vaccine. This increased efficacy was paralleled by higher...
Shigellosis is the leading cause of diarrheal disease, especially in children low- and middle-income countries, often associated with anti-microbial resistance. Currently, there are no licensed vaccines widely available against
Shigellosis is a leading cause of diarrheal disease in low-middle-income countries (LMICs). Effective vaccines will help to reduce the burden, exacerbated by increasing antibiotic resistance, most susceptible population represented young children. A challenge for broadly protective vaccine against shigellosis cover epidemiologically relevant serotypes among >50 Shigella circulating worldwide. The GMMA platform has been proposed as an innovative delivery system O-antigens, and we have...
A new family of dual binding site acetylcholinesterase (AChE) inhibitors has been designed, synthesized, and tested for their ability to inhibit AChE, butyrylcholinesterase (BChE), AChE-induced self-induced β-amyloid (Aβ) aggregation β-secretase (BACE-1), cross the blood-brain barrier. The heterodimers consist a unit racemic or enantiopure huprine Y X donepezil-related 5,6-dimethoxy-2-[(4-piperidinyl)methyl]indane moiety as active peripheral mid-gorge-interacting moieties, respectively,...
Simulating protein flexibility is a major issue in the docking-based drug-design process for which single methodological solution does not exist. In our search of new anti-Alzheimer ligands, we were faced with challenge including receptor plasticity virtual screening campaign aimed at finding β-secretase inhibitors. To this aim, incorporated simulations by using an ensemble static X-ray enzyme structures to screen National Cancer Institute database. A unified description motion was also...