ChatGPT is a Large Language Model (LLM) which allows for natural language processing and interactions with users in conversational style. Since its release 2022, it has had significant impact many occupational fields, including medical education. We sought to gain insight into the extent type of usage at Caribbean school, American University Antigua College Medicine (AUA).We administered questionnaire 87 full-time faculty school via email. quantified made graphical representations results...

The majority of species within the genus Malassezia are lipophilic yeasts that colonize skin warm-blooded animals. Two species, globosa and restricta, implicated in causation seborrheic dermatitis/dandruff (SD/D). During our survey SD/D cases, we isolated several noticed vast variations a few lipid-dependent species. Variations observed phenotypic characteristics (colony morphology, absence catalase activity, growth at 37°C, precipitation surrounding wells containing Tween 20 or Cremophor...

Summary The aetiology of psoriasis remains elusive. Among multiple factors hypothesised, association Malassezia spp. is supported by response to topical antifungals. objective this study was evaluate the with psoriatic lesion. subjects included 50 consecutive patients psoriasis, and age‐ sex‐matched healthy controls. Samples were collected using scotch tape over one square inch area from lesional non‐lesional sites. isolated identified phenotypic methods confirmed ITS2 PCR‐RFLP sequencing...

Purpose. We aimed to evaluate a PCR-based technique for the diagnosis of mucormycosis and identification fungi from fresh tissue specimens in patients with rhino-orbito-cerebral-mucormycosis (ROCM). Methodology. Fifty cases ROCM were included study. Conventional was performed using microscopy culture. Molecular by amplifying ribosomal DNA pan-fungal ITS primers semi-nested Mucorales-specific 18S region. The amplified products sequenced identify agents. utility PCR–RFLP region rDNA evaluated...

: Cervical cancer is the fourth leading cause of cancer-related deaths in women. Human papilloma virus (HPV) known to cervical cancer. The incidence and mortality has drastically reduced due effective vaccination against HPV developed countries. projected rise cases Latin American Caribbean countries necessitates a study evaluate awareness about HPV, cancer, vaccine, prevention among women Antigua Barbuda.

Magnusiomyces capitatus is an emerging opportunistic yeast in the Mediterranean region. We report from Nepal one case of M. infection and six other cases colonization/probable due to at a tertiary care center. Majority patients were immunocompromised, extreme age, associated with comorbidities, had history close contact livestock poultry. The isolates identified by phenotypic genotypic (ITS D1/D2 region 26S rDNA sequence) methods. Molecular typing was carried out amplified fragment length...

In Asian countries, Trichosporon infection is a well-known disease in Japan. India, the increasingly recognised. The study was conducted to characterise clinical isolates from India by phenotypic and molecular techniques. A total of 31 isolates, recovered patients 14 hospitals across were sequenced (ITS IGS1 regions rDNA). vitro drug susceptibility testing performed against amphotericin-B, fluconazole, itraconazole, voriconazole posaconazole. IGS1, rather than ITS sequences, correctly...

The epidemiology of nocardiosis is evolving with increasing number Nocardia spp. causing human infection. In recent years, molecular techniques have been used to identify There are limited data available on the spectrum isolated from clinical samples in India. Here, a study was carried 30 isolates maintained our National Culture Collection evaluate for identifying agents. were identified by sequencing two promising genes: 16S rRNA gene and hsp65. Both hsp65 could reliably 90 % isolates, i.e....

Human infection due to Nocardiopsis, an actinomycete, is rare and the majority of those infections are Nocardiopsis dassonvillei. This agent has been implicated in cutaneous, pulmonary, eye disseminated infections. It never isolated from nose or any nasal infection. We report here a case vestibular abscess N. dassonvillei adult diabetic patient. The bacterium was identified on basis morphological biochemical characteristics, confirmed by sequencing 16S rRNA hsp65 genes. patient successfully...

Malassezia species, which are skin colonizers, being debated as to their pathogenic role in various cutaneous diseases. Species identification of is important particular species have been implicated or associated with specific japonica, a relatively newly described has not completely characterized owing the rarity its isolation. In present study we describe phenotypic and molecular characterization six M. japonica strains isolated from patients psoriasis vulgaris. contrast physiological...

Seborrhoeic dermatitis/dandruff (SD/D) is a common, persistent, relapsing inflammatory condition affecting the areas rich in sebaceous glands. SD/D widely prevalent India but Malassezia species implicated are not well studied. To estimate prevalence and spectrum of causing understand sociodemographic characteristics rural urban populations, total 200 patients 100 healthy controls (HC) from both backgrounds were enrolled this study. severity was clinically graded as mild, moderate, severe,...

Malassezia is a commensal that sometimes becomes pathogenic under the influence of diverse factors. Several species are difficult to culture, making traditional methods identification challenging. The problem with molecular typing in association seborrheic dermatitis/dandruff (SD/D) arises due unavailability these fastidious yeast cultures. aim study was investigate between fluorescent amplified fragment length polymorphism (FAFLP) genotypes, disease state (SD/D), and geographic distribution...

β-Endorphin is known to stimulate phospholipase production by Malassezia pachydermatis during canine dermatoses. The role of β-endorphin in infection humans not well studied. present study compares the influence on globosa and restricta isolated from patients with seborrhoeic dermatitis/dandruff (SD/D) healthy controls. isolates (five each two species controls) were grown modified Dixon's agar or without 100 nmol/L β-endorphin. Phospholipase activity was quantified based its ability...

Pityriasis versicolor (PV) is the most common chronic superficial infection of stratum corneum, reported in 40-60% tropical population. After description new Malassezia species, only a few studies have been conducted from India.Molecular identification, quantification species implicated with PV and correlation to its clinical presentation.The subjects include 50 patients, who attended dermatology outpatient department our hospital healthy individuals. Same size area skin was sampled lesional...