A5018046260- Antifungal resistance and susceptibility
- Bacterial Identification and Susceptibility Testing
- Fungal Infections and Studies
- Plant Pathogens and Fungal Diseases
- Fungal Biology and Applications
- Advanced Cellulose Research Studies
- Infective Endocarditis Diagnosis and Management
- Antimicrobial Resistance in Staphylococcus
- COVID-19 Clinical Research Studies
- Bacterial biofilms and quorum sensing
- COVID-19 Impact on Reproduction
- Probiotics and Fermented Foods
- Immune Cell Function and Interaction
- NF-κB Signaling Pathways
- SARS-CoV-2 and COVID-19 Research
- Natural Antidiabetic Agents Studies
- Helminth infection and control
- Lichen and fungal ecology
- Antibiotic Resistance in Bacteria
- Wound Healing and Treatments
- Nail Diseases and Treatments
- Advances in Cucurbitaceae Research
- Hydrogels: synthesis, properties, applications
- Drug Solubulity and Delivery Systems
- Carbon and Quantum Dots Applications
University of Tennessee Health Science Center
2023-2024
Southeast University
2015-2024
Post Graduate Institute of Medical Education and Research
2014-2024
Bangladesh University of Textiles
2024
Georgetown University
2021-2023
East Carolina University
2021-2022
University of Dhaka
2022
MedStar Georgetown University Hospital
2021-2022
George Institute for Global Health
2021
A mussel mimetic transdermal patch was prepared using bacterial cellulose (BC), a green resource derived from Glucanoacetobacter xylinus (MTCC7795). To impart the property, dopamine (DOPA), catechol-containing compound, used to modify isolated BC via an amidation reaction between carboxylated and DOPA, end product successively characterized by 1H NMR FTIR analysis. The free hydroxyl group of DOPA moiety DOPA-modified (BC-DOPA) utilized prepare BC-DOPA/rGO/Ag NPs, composite film incorporating...
This study was designed to understand the molecular mechanisms of azole resistance in Candida tropicalis using genetic and bioinformatics approaches. Thirty-two azole-resistant 10 azole-susceptible (S) clinical isolates C. were subjected mutation analysis target genes including ERG11. Inducible expression 17 other potentially associated with also evaluated. Homology modeling docking performed effect amino acid alterations mediating resistance. Of 32 resistant isolates, 12 (37.5%) showed...
Abstract Objective Momordica charantia, commonly known as bitter gourd, is traditionally used remedies for various diseases including diabetes. The main objective of this study to investigate the in vitro and vivo insulinotropic antidiabetic effects an 80% ethanolic extract M. charantia (EEMC) fruit, well underlying molecular mechanism involved preliminary phytochemical screening. Methods insulin secretion was measured using clonal pancreatic BRIN-BD11 β-cells isolated mouse islets. ability...
Candida albicans and Staphylococcus aureus are two commonly associated pathogens that cause nosocomial infections with high morbidity mortality. Our prior current work using a murine model of polymicrobial intra-abdominal infection (IAI) demonstrates synergistic lethality is driven by Candida-induced upregulation functional S. α-toxin leading to sepsis organ damage. In order determine the candidal effector(s) mediating enhanced virulence, an unbiased screen C. transcription factor mutants...
With the changing epidemiology and emergence of antifungal resistance among Candida species, rapid susceptibility testing (AFST) is crucial for optimization therapy. This study was conducted to standardize a matrix-assisted laser desorption ionization-time flight mass spectrometry (MALDI -TOF MS) based AFST method (ms-AFST) tropicalis isolates. Clinical isolates C. were confirmed fluconazole by CLSI (M27-A3) method. The incubation period drug concentration optimized determine minimal profile...
Purpose Invasive fungal infections caused by melanized fungi are a growing concern. Rapid and reliable identification plays an important role in optimizing therapy. Matrix‐assisted laser desorption/ionization–time‐of‐flight mass spectrometry (MALDI‐TOF MS)‐based has emerged as faster more accurate diagnostic technique. However, lack of protein extraction protocol limited database restricts the MALDI‐TOF MS. The study is designed to standardize protocol, enrich existing, create in‐house for...
Candida tropicalis arises as one of the predominant non-Candida albicans (NCAC) species causing invasive candidiasis in Asian countries. A rise reports C. with a parallel increase fluconazole resistance has also been observed. The genes and underlying pathways associated azole antifungal is still not properly understood. RT-qPCR most promising approach for expression analysis target to understand mechanisms resistance. reliability reproducibility this technique depend on selection suitable...
To standardize the matrix-assisted laser desorption ionization-time of flight mass spectrometry protocols and expansion existing Bruker Biotyper database for mold identification.Four different sample preparation methods (protocol A, B, C D) were evaluated.On analyzing each protein extraction method, reliable identification best log scores achieved through protocol D. The same was used to identify 153 clinical isolates. Of these 153, 123 (80.3%) accurately identified by using remaining 30...
Increasing incidence of azole resistance in Candida tropicalis, especially to fluconazole, has been seen Asian countries including India. Limited knowledge is available on the molecular mechanisms associated with development C. tropicalis. The present study examined dynamics vitro tropicalis after prolonged treatment fluconazole.Nine fluconazole-susceptible isolates were used this study. Fluconazole was induced experimentally isolates. stability and cross-resistance other azoles examined....
A layer-by-layer (L-B-L) bacterial cellulose (BC)-based transdermal patch has been prepared via a Schiff base reaction. The L-B-L assembly consisting of covalently cross-linked ethylene diamine-modified carboxymethylated BC isolated from the Glucanoacetobacter xylinus (MTCC7795) strain and aldehyde-modified pectin formed presence imine bond assists self-healing process after being scratched in pH 7.4 buffer solution monitored optical microscopy, atomic force tensile strength analyses....
Summary Mucorales are saprobes, ubiquitously distributed and able to infect a heterogeneous population of human hosts. The fungi require robust stress responses survive in host. We tested the growth presence different abiotic stress. Eight pathogenic species Mucorales, including Rhizopus arrhizus, microsporus, Rhizomucor pusillus, Apophysomyces elegans, Licthemia corymbifera, Cunninghamella bertholletiae, S yncephalastrum racemosum Mucor racemosus , were exposed inducers: osmotic (sodium...
Increasing reports of azole resistance in Candida tropicalis , highlight the development rapid detection techniques. Nonsynonymous mutations lanosterol C14 alpha-demethylase ( ERG11 ) gene is one predominant mechanisms C . We evaluated tetra primer-amplification refractory mutation system-PCR (T-ARMS-PCR), restriction site (RSM), and high-resolution melt (HRM) analysis methods for based on polymorphism Twelve azole-resistant 19 susceptible isolates were included. DNA sequencing was performed...
Regulatory T cells (Tregs) are not terminally differentiated but can acquire effector properties. Here we report an increased expression of human endogenous retrovirus 1 (HERV1-env) proteins in Tregs patients with de novo autoimmune hepatitis and hepatitis, which induces endoplasmic reticulum (ER) stress. HERV1-env-triggered ER stress activates all three branches (IRE1, ATF6, PERK) the unfolded protein response (UPR). Our coimmunoprecipitation studies show interaction between HERV1-env ATF6...
ABSTRACT Candida albicans and Staphylococcus aureus are two commonly associated pathogens that cause nosocomial infections with high morbidity mortality. Our prior current work using a murine model of polymicrobial intra-abdominal infection (IAI) uncovered synergistic lethality was driven by -induced upregulation functional S. ⍺-toxin leading to sepsis organ damage. In order determine the candidal effector(s) mediating enhanced virulence, an unbiased screen C. transcription factor mutants...
The clinical profile, molecular characteristics and antifungal susceptibility patterns of the Mucoraceous mould, Syncephalastrum spp are poorly characterized. present study provides a comprehensive overview these aspects this rare fungus. We characterized 8 isolates Syncephalestrum by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF), 28S rDNA sequencing, Amplified Fragment Length Polymorphism (AFLP, n = 7) in-vitro testing. Clinical details eight cases were reviewed...
Accurate and early identification of dermatophytes enables prompt antifungal therapy. However, phenotypic molecular methods are time-consuming. MALDI-TOF MS-based is rapid, but an optimum protocol not available.To develop validate protein extraction for the efficient accurate by MS.Trichophyton mentagrophytes complex (n = 4), T. rubrum 4) Microsporum gypseum were used optimisation protocols. Thirteen different evaluated. A total 125 DNA sequence confirmed clinical isolates to create expand...