A5101880450- Cellular Mechanics and Interactions
- Developmental Biology and Gene Regulation
- Hippo pathway signaling and YAP/TAZ
- Neurobiology and Insect Physiology Research
- Cellular transport and secretion
- Invertebrate Immune Response Mechanisms
- Wnt/β-catenin signaling in development and cancer
- Animal Genetics and Reproduction
- Insect and Pesticide Research
- Plant Reproductive Biology
- CRISPR and Genetic Engineering
- Plant Surface Properties and Treatments
- Cell Adhesion Molecules Research
- Insect and Arachnid Ecology and Behavior
- Endoplasmic Reticulum Stress and Disease
- Plant responses to water stress
- Viral Infectious Diseases and Gene Expression in Insects
- Cytokine Signaling Pathways and Interactions
- Silk-based biomaterials and applications
Nanjing University
2011-2019
Model Animal Research Center
2011-2019
Shangrao Normal University
2019
The major environmental determinants of honeybee caste development come from larval nutrients: royal jelly stimulates the differentiation larvae into queens, whereas beebread leads to worker bee fate. However, these are not fully characterized. Here we report that plant RNAs, particularly miRNAs, which more enriched in than jelly, delay and decrease body ovary size honeybees, thereby preventing queens inducing bees. Mechanistic studies reveal amTOR, a stimulatory gene differentiation, is...
During Drosophila oogenesis, two actin dynamics regulators, cofilin and Rac, are required for the collective migration of a coherent cluster cells called border cells. Cell culture data have shown that Rac both essential lamellipodium formation, but signaling results in phosphorylation hence inactivation cofilin. So it remains unclear whether plays promoting or inhibitory role during cell migration. We show here is F-actin turnover lamellipodial protrusion Interestingly, reducing dosage by...
Apical-basal polarity is a hallmark of epithelia and it needs to be remodeled when epithelial cells undergo morphogenetic cell movements. Here, we use border in Drosophila ovary address how the apical-basal turned into front-back, inside-outside polarities, during collective migration. We find that Crumbs (Crb) complex required for generation three distinct but inter-connected polarities cells. Specifically, Crb complex, together with Par endocytic recycling machinery, ensures strict...
During epithelial morphogenesis, cells not only maintain tight adhesion for integrity but also allow dynamic intercellular movement to take place within cell sheets. How these seemingly opposing processes are coordinated is well understood. Here, we report that the actin disassembly factors AIP1 and cofilin required remodeling of adherens junctions (AJs) during ommatidial precluster formation in Drosophila eye epithelium, a highly stereotyped rearrangement process which describe detail our...
During collective migration, guidance receptors signal downstream to result in a polarized distribution of molecules, including cytoskeletal regulators and themselves, response an extracellular gradient chemotactic factors. However, the underlying mechanism asymmetry generation context migration group cells is not well understood. Using border Drosophila ovary as model system for we found that receptor tyrosine kinase (RTK) PDGF/VEGF (PVR) required recycling endosome exocyst leading cell...
Collective migration of loosely or closely associated cell groups is prevalent in animal development, physiological events, and cancer metastasis. However, our understanding the mechanisms collective incomplete. Drosophila border cells provide a powerful vivo genetic model to study identify essential genes for this process. Using cell-specific RNAi-silencing Drosophila, we knocked down 360 conserved signaling transduction adult flies pathways migration. We uncovered plethora genes, large...
Abstract Apical-basal polarity plays critical roles in the functions of epithelial tissues. However, mechanisms establishment and maintenance remain to be fully elucidated. Here we show that membrane-associated guanylate kinase (MAGUK) family protein Dlg5 is required for apical follicle epithelium during Drosophila oogenesis. localizes at membrane adherens junction (AJ) early stage egg chambers. Specifically, demonstrate major function promote localization Crumbs, since overexpression Crumbs...
Collective migration plays critical roles in animal development, physiological events, and cancer metastasis. However, the molecular mechanisms of collective cell are not well understood. Drosophila border cells represent an excellent vivo genetic model to study identify novel regulatory genes for migration. Using Mosaic Analysis with a Repressible Cell Marker (MARCM) system, we screened 240 P-element insertion lines essential Two were uncovered, including dlg5 (discs large 5) CG31689....
The Drosophila transgenic technology and fluorescent protein fusions are powerful tools to analyze expression patterns, subcellular localization dynamics. Recently, the has been improved by highly efficient phiC31 site-specific integration system. Many new proteins with desirable advantages have developed. However, system newly developed not systematically applied in vectors. Here, we constructed a modular toolset of C-terminal fusion vectors based on for generation lines. These cloning...
During collective migration, guidance receptors signal downstream to result in a polarized distribution of molecules, including cytoskeletal regulators and themselves, response an extracellular gradient chemotactic factors. However, the underlying mechanism asymmetry generation context migration group cells is not well understood. Using border Drosophila ovary as model system for we found that receptor tyrosine kinase (RTK) PDGF/VEGF (PVR) required recycling endosome exocyst leading cell...