A5074878459- Cutaneous Melanoma Detection and Management
- Melanoma and MAPK Pathways
- Epigenetics and DNA Methylation
- Cancer Immunotherapy and Biomarkers
- Cancer Cells and Metastasis
- Hedgehog Signaling Pathway Studies
- Cancer-related Molecular Pathways
- Cell Adhesion Molecules Research
- RNA and protein synthesis mechanisms
- Cancer-related molecular mechanisms research
- Cell death mechanisms and regulation
- RNA Interference and Gene Delivery
- Glutathione Transferases and Polymorphisms
- Genomics and Phylogenetic Studies
- Reproductive Biology and Fertility
- Cancer Genomics and Diagnostics
- Cancer Mechanisms and Therapy
- Single-cell and spatial transcriptomics
- Cancer-related gene regulation
- Viral Infections and Outbreaks Research
- Ovarian function and disorders
- Lipid metabolism and disorders
- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- Molecular Biology Techniques and Applications
Hospital de Câncer de Barretos
2015-2025
Centre International de Recherche sur le Cancer
2022-2023
Universidade do Porto
2015
BRAF inhibitor (BRAFi) therapy for melanoma patients harboring the V600E mutation is initially highly effective, but almost all relapse within a few months. Understanding molecular mechanisms underpinning BRAFi-based therefore an important issue. Here we identified previously unsuspected mechanism of BRAFi resistance driven by elevated Hedgehog (Hh) pathway activation that observed in cohort after vemurafenib treatment. Specifically, demonstrate cell lines, with acquired vitro-induced...
BRAF mutations are known drivers of melanoma development and, recently, were also described as players in the Warburg effect, while this reprogramming energy metabolism has been identified a possible strategy for treating patients. Therefore, aim work was to evaluate expression and prognostic value panel glycolytic metabolism-related proteins series melanomas. The immunohistochemical MCT1, MCT4, GLUT1, CAIX evaluated 356 patients presenting 20 benign nevi. Samples included nevi, 282 primary...
Acral lentiginous melanoma (ALM) is the less common subtype with singular characterization. TERT (human telomerase reverse transcriptase) promoter mutations have being described as recurrent in melanomas and infrequent ALM, but their real incidence clinical relevance unclear. The objectives of this study were to describe prevalence correlate molecular profile other drive genes features. Sixty-one samples from 48 patients ALM analyzed. After DNA isolation, mutation profiles hotspot region...
Homeobox A9 promoter methylation (HOXA9) has been reported as a biomarker for early lung adenocarcinoma patients' prognosis. We aim to evaluate its prognostic value, regardless of disease stage. Using droplet digital PCR, we measured HOXA9 in cohort comprising 161 Brazilian patients. Low was associated with higher cancer-specific survival but showed no significance after adjustment clinical covariates. While low earlier stages, association observed this subset Overall, is not an independent...
Abstract Ultraviolet radiation (UV) is causally linked to cutaneous melanoma, yet the underlying epigenetic mechanisms, known as molecular sensors of exposure, have not been characterized in clinical biospecimens. Here, we integrate clinical, epigenome (DNA methylome), genome and transcriptome profiling 112 melanoma from two multi-ethnic cohorts. We identify UV-related alterations regulatory regions immunological pathways, with multi-OMICs cancer driver potential affecting patient survival....
Mutation testing of the key genes involved in melanoma oncogenesis is now mandatory for application targeted therapeutics. However, knowledge mutational profile remains largely unknown Brazil.In this study, we assessed mutation status driver BRAF, NRAS, TERT, KIT, and PDGFRA a cohort 459 patients attended at Barretos Cancer Hospital between 2001 2012. We used polymerase chain reaction followed by Sanger sequencing to analyze hot spot mutations BRAF exon 15 (V600E), NRAS (codons 12/13 61),...
Melanin is produced by melanocytes and protects against DNA damage ultraviolet light. Unfortunately, the melanin protein present in melanoma tumor cells often co-purified during extraction, this contamination may inhibit subsequent PCR methods, which directly impacts research applications molecular diagnostic tests needed for targeted therapeutics. There are presently no described purification protocols that efficiently remove from genomic DNA. In study, we compare six different methods...
Waterpipe smoking is frequent in the Middle East and Africa with emerging prevalence worldwide. The epigenome acts as a molecular sensor to exposures crucial driver several diseases. With widespread use of waterpipe smoking, it timely investigate its epigenomic markers their role addiction, central player disease prevention therapeutic strategies. DNA methylome-wide profiling was performed on an exposure-rich population from East, constituting 216 blood samples split equally between never,...
Apoptosis induction has emerged as a treatment option for anticancer therapy. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), type II transmembrane protein, is potent and specific pro-apoptotic protein ligand, which activates the extrinsic apoptosis pathway of cell death receptors. Here we describe construction characterization new soluble TRAIL, sfTRAIL, stabilized with trimerization Foldon domain from Fibritin bacteriophage T4. Supernatants 0.22 μM-filtered supernatants...
Abstract Melanoma represents the deadliest of all skin cancers being responsible for more than 75% cancer-related deaths. Although results with BRAF inhibitors have been very promising, practically patients treated thus far developed resistance to these drugs. The Hedgehog (Hh) signaling pathway has implicated in a variety malignancies, including melanoma. However, there are no reports on activation this setting vemurafenib-resistance. Herein, we first identified that melanoma cell lines,...
Abstract Ultraviolet radiation (UV) is causally linked to cutaneous melanoma, yet the underlying epigenetic mechanisms, known as molecular sensors of exposure, have never been characterized in clinical biospecimen. Here, we integrate and epigenome (DNA methylome), genome transcriptome profiling 112 melanoma from two multi-ethnic cohorts. We identify UV-related alterations regulatory regions immunological pathways, with multi-OMICs cancer driver potential affecting patient survival. TAPBP ,...
The polycystic ovary syndrome is the most common endocrine disorder, characterized by dysregulation of ovarian angiogenesis. This alteration can be related to changes in activities vascular endothelial growth factor (VEGF) gene. Single-nucleotide polymorphisms have been observed promoter, intronic, and untranslated regions VEGF gene, several studies suggested that these may associated with risk syndrome. study aimed investigate association between rs2010963 rs833061 haplotypes etiology...