A5038009298- Cancer Cells and Metastasis
- Lung Cancer Treatments and Mutations
- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Cancer, Hypoxia, and Metabolism
- Testicular diseases and treatments
- Cancer Immunotherapy and Biomarkers
- Epigenetics and DNA Methylation
- Cancer-related Molecular Pathways
- Colorectal Cancer Treatments and Studies
- Head and Neck Cancer Studies
- HER2/EGFR in Cancer Research
- BRCA gene mutations in cancer
- Hedgehog Signaling Pathway Studies
- Sarcoma Diagnosis and Treatment
- Melanoma and MAPK Pathways
- Chromosomal and Genetic Variations
- Cervical Cancer and HPV Research
- Genetics and Neurodevelopmental Disorders
- Genomic variations and chromosomal abnormalities
- Genital Health and Disease
- PI3K/AKT/mTOR signaling in cancer
Hospital de Câncer de Barretos
2016-2025
Hospital São Paulo
2025
Zero to Three
2020
Universidade de São Paulo
2012
To better understand the immune microenvironment of pancreatic ductal adenocarcinomas (PDACs), here we explored relevance T and B cell compartmentalisation into tertiary lymphoid structures (TLSs) for generation local antitumour immunity.We characterised functional states spatial organisation PDAC-infiltrating cells using single-cell RNA sequencing (scRNA-seq), flow cytometry, multicolour immunofluorescence, gene expression profiling microdissected TLSs, as well in vitro assays. In addition,...
Abstract BRAF , NRAS and TERT mutations occur in more than 2/3 of melanomas. Its detection patient’s blood, as circulating tumor DNA (ctDNA), represents a possibility for identification monitoring metastatic disease. We proposed to standardize liquid biopsy platform identify hotspot plasma samples from advanced melanoma patients investigate whether it was associated clinical outcome. Firstly, we performed digital polymerase chain reaction using cell lines validation determination limit (LOD)...
// Lidia Maria Rebolho Batista Arantes 1 , Ana Carolina Laus Matias Eliseo Melendez de Carvalho Bruna Pereira Sorroche Pedro Rafael Martins De Marchi 2 Adriane Feijó Evangelista Cristovam Scapulatempo-Neto 3 Luciano Souza Viana André Lopes 1, 4 Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos, SP, Brazil Department of Clinical Oncology, Pathology, Head and Neck Surgery, Correspondence to: Carvalho, email: carvalhoal@gmail.com Keywords: HNSCC, chemoradiation, microRNA...
Head and neck squamous cell carcinoma (HNSCC) is the sixth most common type of cancer. The majority patients present advanced stage disease has poor survival. Therefore, it imperative to search for new biomarkers alternative effective treatment options. Most cancer cells rely on aerobic glycolysis generate energy metabolic intermediates. This phenotype a hallmark cancer, characterized by an increase in glucose consumption production high amounts lactate. Consequently, need up-regulate many...
Background The molecular non-genetic changes of resistance to sotorasib are currently uncertain. aim this study was generate a sotorasib-resistant cell line via selective pressure and systematically examine the phenotypic alterations caused by resistance. Methods Mutant NCI-H358 (KRAS G12C ) were exposed incremental doses (2–512 nM) sotorasib. Then, resistant clones separated single-cell sorting. Proliferation analyzed in real-time xCELLigence; protein profiles quantified arrays; mRNA...
Ductal carcinoma in situ is a non-obligate precursor of invasive breast and presents potential risk over or undertreatment. Finding molecular biomarkers disease progression could allow for more adequate patient treatment. We aimed to identify that can predict invasiveness risk.In this epithelial cell-based study archival formalin-fixed paraffin-embedded blocks from six patients diagnosed with lesions (pure ductal carcinoma), in-situ situ), synchronous (invasive an component) three...
Abstract Current diagnostic tests for tuberculosis (TB) are not able to predict reactivation disease progression from latent TB infection (LTBI). The main barrier predicting is the lack of our understanding host biomarkers associated with active disease. Here, we applied an immune-based gene expression profile by NanoString platform identify whole blood markers that can distinguish other lung diseases (OPD), and could be further evaluated as a predictor. Among 23 candidate genes...
One-hundred-six patients diagnosed with non-muscle invasive bladder cancer and treated intravesical BCG were included divided into two groups, BCG-responsive (n = 47) -unresponsive 59). Immunohistochemistry was used to evaluate PD-L1 expression MSI assessed by a commercial multiplex PCR kit. The mRNA profile of 15 immune checkpoints performed using the nCounter technology. For in silico validation, distinct cohorts sourced from Gene Expression Omnibus (GEO) database used.
Abstract Cell-free DNA is present in different biological fluids and when released by tumor cells may contribute to pro-tumor events such as malignant transformation of adjacent the metastasis. Thus, this study analyzed effect cell-free DNA, isolated from blood prostate cancer patients, on non-tumor cell lines (RWPE-1 PNT-2). To achieve this, we performed quantification characterization assays, evaluation gene miRNA expression profiling focused progression EMT, metabolomics mass spectrometry...
Cancer development by the human papillomavirus (HPV) infection can occur through canonical HPV/p53/RB1 pathway mediated E2/E6/E7 viral oncoproteins. During transformation process, HPV inserts its genetic material into host Integration Sites (IS), affecting coding genes and miRNAs. In penile cancer (PeCa) there is limited data on miRNAs that regulate mRNA targets associated with HPV, such as TP53 RB1 genes. Considering high frequency of in PeCa patients Northeast Brazil, global miRNA...
Background: EGFR mutations are present in approximately 15−50% of non-small cell lung cancer (NSCLC), which predictive anti-EGFR therapies. At variance, NSCLC patients harboring KRAS resistant to those approaches. Afatinib and allitinib second-generation pan-EGFR drugs, yet no biomarkers known the context. In study, we evaluated efficacy inhibitors a panel 15 lines associated with phenotype. Methods: wild-type sensitive NCI-H292 line was further transfected (p.G12D p.G12S). The inhibitors’...
microRNAs (miRNAs) are small endogenous noncoding RNAs, and alterations in their expression may contribute to oncogenesis. Discovering a unique miRNA pattern holds the potential for early detection novel treatment possibilities cancer. This study aimed evaluate pediatric patients with gonadal germ cell tumors (GCTs), focusing on characterizing profiles of each histological subtype identifying distinct signature total 42 samples GCTs. The analysis revealed all types, regardless primary site....
Background: Testicular germ cell tumors (TGCTs), a group of heterogeneous neoplasms, are the most frequent teenagers and young men, with incidence rising worldwide. High cure rates can be achieved through cisplatin (CDDP)-based treatment, but approximately 10% patients present refractory disease virtually no treatment alternatives. Here, we explored new strategies to treat CDDP-resistant. Methods: In vitro TGCT CDDP-resistance model was established differential mRNA expression profiles were...
Abstract Background Oropharyngeal squamous cell carcinomas (OpSCCs) are commonly associated with high rates of treatment failure. Objectives To evaluate methylation‐based markers in plasma from OpSCC patients as emerging tools for accurate/noninvasive follow‐up. Methods Pretreatment formalin‐fixed paraffin‐embedded (FFPE) biopsies (n = 52) and paired 15) were tested the methylation CCNA1 , DAPK CDH8 TIMP3 by droplet digital PCR (ddPCR). Results Seventy‐one percent (37/52) showed at least one...
The molecular profile of endometrial cancer has become an important tool in determining patient prognosis and their optimal adjuvant treatment. In addition to Cancer Genome Atlas (TCGA), simpler tools have been developed, such as the Proactive Molecular Risk Classifier for Endometrial (ProMisE). We attempted determine a genetic signature build recurrence risk score patients diagnosed with low- intermediate-risk cancer.A case-control study was conducted. eligible were women between January...
This study aimed to evaluate the association between self-reported race/color and ancestry in Brazilian patients with breast cancer.This was an observational, transversal, epidemiological study, evaluating race 1,127 cancer. For genetic ancestry, a 46-AIM-INDEL panel used. The ancestral profile evaluated Structure v.2.3.3 software. Descriptive statistics were performed. To assess differences analysis of variance Bonferoni adjustment used.The distribution 77.7% white, 17.6% brown, 4.1% black,...
Melanin is produced by melanocytes and protects against DNA damage ultraviolet light. Unfortunately, the melanin protein present in melanoma tumor cells often co-purified during extraction, this contamination may inhibit subsequent PCR methods, which directly impacts research applications molecular diagnostic tests needed for targeted therapeutics. There are presently no described purification protocols that efficiently remove from genomic DNA. In study, we compare six different methods...
Metastatic lymph node involvement influences therapy decisions and serves as a prognostic indicator in oral squamous cell carcinoma (OSCC). However, many early-stage patients with clinically negative nodes exhibit no metastasis upon surgical staging. This study aimed to identify differentially expressed miRNAs capable of distinguishing pathologically positive (pN+) from (pN0) OSCC without clinical evidence metastases (cN0).Expression levels 798 were assessed tumor samples 10 pN+ pN0 using...
The Barretos Cancer Hospital Animal Facility (BCHAF) is a unique facility in Brazil exclusively dedicated to working with animal models for cancer research. In this article, we briefly present our modern and the main experiments performed, focusing on mutant strains of mice (PTCH-knockout ApcMin mice), xenograft models, patient-derived xenografts (PDXs). Our results show progress challenges establishing these need having an appropriate representation population better understand tumor...