Equine-chorionic gonadotropin (eCG) is widely used in fixed-time artificial ovulation and superovulation protocols to improve reproductive performance. Commercial products available the market for veterinary use consist of partially purified preparations pregnant mare serum (PMSG). eCG a heterodimeric glycoprotein thus extremely challenging quantify through chromatography. Pharmaceutical companies usually carry out vivo methods measure potency finished containing eCG. Considering three Rs...

Usnic acid is one of the most common and abundant metabolites found in various lichen genera, which are important sources biologically active compounds. The aim this study was to evaluate genotoxic antigenotoxic potential (+)-usnic (UA) by micronucleus comet assays V79 cell cultures Swiss mice. For assessment genotoxicity, cells were treated with 15, 30, 60, 120μg/mL UA, established based on clonogenic efficiency cytotoxic assay. mice UA doses 25, 50, 100, 200mg/kg body weight. same...

ABSTRACT Copaifera lansdorffii Desf. is known as ‘copaíba’, ‘copaiva’ or ‘paú‐de‐óleo’, and found in part of Brazil. The present study was undertaken to evaluate the genotoxic potential C . langsdorffii leaf hydroalcoholic extract (CLE) its influence on genotoxicity induced by chemotherapeutic agent doxorubicin (DXR) using Swiss mouse peripheral blood micronucleus test. HPLC analysis CLE two monolithic columns linked series allowed quantification major flavonoid heterosides, quercitrin...

Solanum lycocarpum St.-Hil (Solanaceae) is a hairy shrub or small much-branched tree of the Brazilian Cerrado, popularly known as "fruit-of-wolf". Considering that induction chromosomal mutations involved in process carcinogenesis, and S. often used folk medicine, it becomes relevant to study its effect on genetic material. In this sense, aim present was determine possible cytotoxic, genotoxic antigenotoxic potentials fruits glycoalkaloid extract (SL) Chinese hamster lung fibroblasts (V79...

Rosmarinic acid (RA) is a polyphenolic compound that shows number of interesting biological activities, such as antiapoptotic, antifibrotic, antioxidant, hepatoprotective, antineurodegenerative, and anti-inflammatory properties. The aim this study was to investigate the ability RA prevent 1,2-dimethylhydrazine (DMH)-induced primary DNA damage aberrant crypt foci (ACF) in Wistar rat colon. animals were treated by gavage with doses 4, 8, 16 mg/kg body weight/day. Next, received single...

This study evaluated the antiproliferative activity of Brazilian green propolis and <i>Baccharis dracunculifolia</i> extracts their major compounds artepillin C baccharin in different tumor cell lines. The lowest IC₅₀ values observed for <i>B. were 41.0 ± 4.5 µg/mL U343 44.9 7.1 HepG2, respectively. Regarding baccharin, 20.1 2.9 13.0 1.5 B16F10, For association plus result was 35.2 0.5 B16F10. Artepillin more cytotoxic than both extracts. No additive or synergistic effect baccharin.

Abstract Baccharis dracunculifolia (Asteraceae), the main botanical source of green propolis, is a shrub Brazilian ‘cerrado’. In folk medicine it used as an anti‐inflammatory agent, mainly for treatment gastrointestinal diseases. The aim present study was to evaluate genotoxic and antigenotoxic effects B. ethyl acetate extract (Bd‐EAE) on Chinese hamster lung fibroblasts (V79 cells) by comet assay. Methyl methanesulfonate (MMS; 200 μ M ) inducer DNA damage. Genotoxicity evaluated using four...

Baccharis dracunculifolia is a plant native from Brazil, commonly known as 'Alecrim-do-campo' and 'Vassoura' used in alternative medicine for the treatment of inflammation, hepatic disorders stomach ulcers. Previous studies reported that artepillin C (ArtC, 3-{4-hydroxy-3,5-di(3-methyl-2-butenyl)phenyl}-2(E)-propenoic acid), main compound interest leaves. This study was undertaken to assess mutagenic effect ethyl acetate extract B. leaves (Bd-EAE: 11.4-182.8 µg/plate) ArtC (0.69-10.99 by...

The fruits of Solanum lycocarpum, known as wolf-fruit, are used in folk medicine, and because that we have evaluated both the genotoxic potential its glycoalkaloidic extract (SL) influence on genotoxicity induced by methyl methanesulfonate. Furthermore, blocking effect SL intake initial stage colon carcinogenesis Wistar rats was investigated a short-term (4-week) bioassay using aberrant crypt foci (ACF) biomarker. Swiss mice peripheral blood micronucleus test. animals were treated with...

(-)-Cubebin (CUB) is a lignan isolated from dry seeds of Piper cubeba. We aimed to assess its genotoxic potential and influence on chromosomal damage (frequency micronuclei - MN) induced by doxorubicin (DXR) in V79 cells urethane (URE) somatic Drosophila melanogaster cells. Our findings indicate an absence CUB-mediated effect at the concentrations tested. The results also revealed that CUB significantly reduced frequency MN DXR, with mean reduction 63.88%. In previous study, our research...

Corrected by: Erratum for: Antimutagenic Potential of Solanum lycocarpum against Induction Chromosomal Aberrations in V79 Cells and Micronuclei Mice by DoxorubicinPlanta Med 2011; 77(13): E13-E13DOI: 10.1055/s-0031-1280192

Color Index (C.I.) Disperse Red 1 (DR1) is a widely used textile azo dye found in rivers. As it may not be completely removed by conventional treatments, humans can exposed through drinking water. Studies have supported vitro toxicity and mutagenicity of commercial DR1. This study aimed to investigate the mutagenic toxicogenomic effects DR1 multiple tissues/organs Swiss male mice. For that, animals were orally (by gavage), at single doses 0.0005, 0.005, 0.5, 50, or 500 mg/kg bw. The two...

Baccharis dracunculifolia De Candole (Asteraceae), a native plant from the Brazilian "cerrado", is widely used in folk medicine as an anti-inflammatory agent and for treatment of gastrointestinal diseases. B. has been described most important source propolis southeastern Brazil, which called green due to its color. The aim present study was evaluate mutagenic antimutagenic effects ethyl acetate extract leaves (Bd-EAE) on Chinese hamster ovary cells. On one hand, results showed significant...

Background: EGFR mutations are present in approximately 15−50% of non-small cell lung cancer (NSCLC), which predictive anti-EGFR therapies. At variance, NSCLC patients harboring KRAS resistant to those approaches. Afatinib and allitinib second-generation pan-EGFR drugs, yet no biomarkers known the context. In study, we evaluated efficacy inhibitors a panel 15 lines associated with phenotype. Methods: wild-type sensitive NCI-H292 line was further transfected (p.G12D p.G12S). The inhibitors’...

The development of natural oil-based cosmetic and pharmaceutical products presents great scientific commercial interest. Herein, we aimed to extract green coffee oil from Arabic by a sustainable cold-pressing method. Furthermore, this work characterize the obtained Fourier-Transform Infrared (FT–IR) ultraviolet–visible spectroscopies (UV–Vis), peroxide analysis, fatty acids profile gas chromatography–mass spectrometry (GC–MS). Moreover, functional biological properties green-coffee product...

Abstract Cervical cancer is the fourth most common in women. Although cure rates are high for early stage disease, clinical outcomes advanced, metastatic, or recurrent disease remain poor. To change this panorama, a deeper understanding of cervical biology and novel study models needed. Immortalized human cell lines such as HeLa constitute crucial scientific tools, but there few other available, limiting our known its molecular heterogeneity. This aimed to establish derived from Brazilian...

Baccharis dracunculifolia (Asteraceae), the main botanical source of green propolis, also known as 'alecrim-do-campo' and 'vassourinha', is a shrub Brazilian 'cerrado' native to South Southeast Brazil. The effects B. ethyl acetate extract (Bd-EAE) were evaluated on 1,2-dimethylhydrazine (DMH)-induced DNA damage aberrant crypt foci (ACF) in colon male Wistar rats by comet ACF assays, respectively. animals treated gavage with doses 6, 12, 24 mg/kg body weight/day. Animals administered single...

Copaifera langsdorffii Desf. is a plant found in South America, especially Brazil. Oleoresin and the leaves of this used as popular medicinal agent. However, few studies on chemical composition aerial parts related biological activities are known. This study aimed to examine cytotoxic, genotoxic, antigenotoxic potential C. hydroalcoholic extract (CLE) two its major compounds afzelin quercitrin. The cytotoxic CLE was determined follows: 1) against genotoxicity induced by doxorubicin (DXR) or...

Betulinic acid (BA) is a pentacyclic triterpene that can be isolated from many medicinal plants around the world. The aim of this study was to evaluate genotoxic potential BA and its effect on genotoxicity induced by different mutagens in V79 cells using cytokinesis-block micronucleus assay. Different concentrations were combined with methyl methanesulfonate (MMS), doxorubicin (DXR), camptothecin (CPT), etoposide (VP-16). frequencies micronuclei cultures treated did not differ those negative...