A5078877220- Potato Plant Research
- Phytochemistry Medicinal Plant Applications
- Cassava research and cyanide
- Research on Leishmaniasis Studies
- Essential Oils and Antimicrobial Activity
- Trypanosoma species research and implications
- Sesquiterpenes and Asteraceae Studies
- Carcinogens and Genotoxicity Assessment
- Botanical Research and Applications
- Parasites and Host Interactions
- 3D Printing in Biomedical Research
- Plant tissue culture and regeneration
- Natural product bioactivities and synthesis
- Tissue Engineering and Regenerative Medicine
- Phytase and its Applications
- Single-cell and spatial transcriptomics
- Advances in Cucurbitaceae Research
- Parasite Biology and Host Interactions
- Bladder and Urothelial Cancer Treatments
- Insect Pest Control Strategies
- Sesame and Sesamin Research
- Plant Pathogens and Resistance
- Patient Safety and Medication Errors
- Cardiac Arrhythmias and Treatments
- Morinda citrifolia extract uses
University of Florida
2021-2024
Kiel University
2024
José R. Reyes Memorial Medical Center
2023
Centro Hospitalar Lisboa Norte
2023
Universidade de São Paulo
2013-2022
Florida Agricultural and Mechanical University
2019-2020
Universidade Federal de Juiz de Fora
2007-2012
Universidade de Ribeirão Preto
2012
University of New Mexico
2007
Hospital Universitário da Universidade de São Paulo
2007
Introduction: Cell-based assays using three-dimensional (3D) cell cultures may reflect the antitumor activity of compounds more accurately, since these models reproduce tumor microenvironment better. Methods: Here, we report a comparative analysis behavior in two most widely employed methods for 3D spheroid culture, forced floating (Ultra-low Attachment, ULA, plates), and hanging drop (HD) methods, RT4 human bladder cancer line as model. The morphology parameters growth/metabolism spheroids...
Abstract 3D bioprinting improves orientation of in vitro tumor models by offering layer positioning cancer cells and associated fibroblasts (CAFs) which can replicate microenvironment. Aim this study was to develop a sodium alginate -gelatin (SA-GL) hydrogel optimizing rheological parameters print non-small cell lung (NSCLC) patient derived xenograft (PDX) CAFs co-cultures. SA-GL hydrogels were prepared, properties evaluated. Both the mixed with printed using INKREDIBLE bioprinter. Hydrogels...
Single-cell transcriptome analysis has been extensively applied in humans and animal models to uncover gene expression heterogeneity between the different cell types of a tissue or an organ. It demonstrated its capability discover key regulatory elements that determine fate during developmental programs. requires isolation labeling messenger RNA (mRNA) derived from each cell. These challenges were primarily addressed mammals by developing microfluidic-based approaches. For plant species...
The X-linked McLeod neuroacanthocytosis syndrome (MLS) has originally been denoted as 'benign' myopathy. We assessed the clinical findings and muscle pathology in eponymous index patient, Hugh McLeod, nine additional MLS patients. Only one patient had manifested with neuromuscular symptoms. During a mean follow-up of 15 years, however, eight patients including initial showed elevated skeletal creatine kinase levels ranging from 300 to 3000 U/L, developed weakness atrophy. Two disabling leg...
Abstract Leishmaniasis is an infection caused by a protozoan parasite of the genus Leishmania and second most prevalent parasitic protozoal disease after malaria in world. We report vitro leishmanicidal activity on promastigote forms amazonensis cytotoxicity, using LLCMK 2 cells, glycoalkaloids from fruits Solanum lycocarpum , determined colorimetric methods. The alkaloidic extract was obtained acid‐base extraction; solamargine solasonine were isolated silica‐gel chromatography, followed...
ABSTRACT Little has been done during the past 100 years to develop new antileishmanial drugs. Most infected individuals live in poor countries and have a low cash income be attractive targets pharmaceutical corporations. Two heterosidic steroids, solamargine solasonine, initially identified as major components of Brazilian plant Solanum lycocarpum , were tested for leishmanicidal activity. Both alkaloids killed intracellular extracellular Leishmania mexicana parasites more efficiently than...
The chemical composition, analgesic and anti-inflammatory properties of essential oil from Ageratum fastigiatum were investigated. main compounds found in the germacrene D, α-humulene β-cedrene. oil, with LD50 2.50 g/kg, inhibited acetic acid-induced writhing at dose 200 mg/kg. In formalin test, first phase (200 mg/kg) second (100 mg/kg mg/kg). hot plate after 30 60 min treatment doses 100 increased reaction time. antiedematogenic effect, reduction on exudate volume leukocyte mobilization...
Expression of the transmembrane protein PD-L1 is frequently upregulated in cancer. Because PD-L1-expressing cells can induce apoptosis or anergy T lymphocytes through binding to PD1 receptor, PD-L1-mediated inhibition activated PD1+ considered a major pathway for tumor immune escape. However, mechanisms that regulate expression microenvironment are not fully understood. Analysis organotypic tissue slice cultures, obtained from mice with implanted syngeneic tumors (MBT2 bladder C3H mice,...
Solanum lycocarpum (Solanaceae) is native to the Brazilian Cerrado. Fruits of this species contain glycoalkaloids solasonine (SN) and solamargine (SM), which display antiparasitic anticancer properties. A method has been developed for extraction HPLC-UV analysis SN SM in different parts S. lycocarpum, mainly comprising ripe unripe fruits, leaf, stem. This analytical was validated gave good detection response with linearity over a dynamic range 0.77-1000.00 μg mL(-1) recovery 80.92-91.71%,...
Abstract Objective This study proposed to use the nanotechnology deliver glycoalkaloidic extract (AE) bladder cancer cells, evaluating their activity in 2D and 3D models biological mechanism of cell death. Methods NPs were prepared by nanoprecipitation method using polylactic acid (PLA) characterized considering size, charge, particle concentration stability. The cytotoxicity was evaluated model, apoptosis cycle investigated flow cytometry. Key findings loading AE (NP-AE) had diameter around...
Melanoma is the most aggressive type of skin cancer, and thus it important to develop new drugs for its treatment. The present study aimed examine antitumor effects solamargine a major alkaloid heteroside in Solanum lycocarpum fruit. In addition was incorporated into nanoparticles (NP) yttrium vanadate functionalized with 3-chloropropyltrimethoxysilane (YVO4:Eu3+:CPTES:SM) determine activity. anti-melanoma assessment performed using syngeneic mouse melanoma model B16F10 cell line. addition,...
O presente trabalho investigou as propriedades antinociceptiva, antiinflamatória e antiulcerogênica do extrato etanólico dos galhos de E. erythropappus. Foram realizados os testes contorções abdominais induzidas por ácido acético; tempo da lambida pata induzida formalina; placa quente; edema induzido carragenina; lesões ulcerativas indometacina etanol. As doses 50, 100 200 mg/kg inibiram em 43,07%, 69,34% 70,07%, respectivamente. foi reduzido ambas fases nas testadas, enquanto o latência...
Schistosomiasis is a chronic disease caused by trematode flatworms of the genus Schistosoma; it accounts for more than 280,000 deaths annually. In this work we investigated effect alkaloidic extract obtained acid-base extraction dried fruits Solanum lycocarpum on schistosomiasis. We used at concentrations 10, 20, and 40 mg/kg to treat mice infected with Schistosoma mansoni in different phases parasite cycle, compared its that positive control praziquantel (60 mg/kg). evaluated results basis...
Cernumidine (CER) is a guanidinic alkaloid isolated from Solanum cernuum leaves. In this work, we investigated the cytotoxicity, chemosensitizing effect of cernumidine to cisplatin (cDDP) and possible mechanism action combination on bladder cancer cells. showed cytotoxicity could sensitize cells cisplatin. The CER+cDDP inhibited cell migration T24 down-regulated MMP-2/9 p-ERK1/2, while it increased EGFR activity corroborating observed inhibition. Down-regulation Bcl-2 up-regulation...