Compelling evidence supports the role of oxidative stress and renal interstitial inflammation in pathogenesis hypertension. Resveratrol is a polyphenolic stilbene, which can lower by activating transcription factor nuclear factor-E 2 -related factor-2 (Nrf2), master regulator numerous genes encoding antioxidant phase II-detoxifying enzymes molecules. Given hypertension, we conducted this study to test hypothesis that long-term administration resveratrol will attenuate and, hence, progression...

Oxidative stress plays a pathogenic role in hypertension, particularly the one associated with diabetes and obesity. Here, we test hypothesis that renal dopamine D1 receptor dysfunction obese Zucker rats is caused by oxidative stress. One group each from lean received tempol, superoxide dismutase mimetic drinking water for 2 weeks. Obese animals were hypertensive, hyperglycemic, hyperinsulinemic, exhibited stress, increased protein kinase C activity. Also, there was hyperphosphorylation of...

Reactive oxygen species have emerged as important molecules in cardiovascular dysfunction such diabetes and hypertension. Recent work has shown that oxidative stress angiotensin II signaling mutually regulate each other by multiple mechanisms contribute to the development of Most known biological actions can be attributed AT1 receptors. The present study was carried out investigate role renal receptor stress-mediated Male Sprague-Dawley rats received tap water (control) or 30 mM l-buthionine...

Age is one of the major risk factors for development chronic pathologies, including kidney diseases. Oxidative stress and mitochondrial dysfunction play a pathogenic role in aging disease. Transcription factor NRF2, master regulator redox homeostasis, altered during aging, but exact implications NRF2 signaling on age-related renal impairment are not yet clear. Herein, we investigated sulforaphane, well-known activator, dysfunction. Young (2-4 month) aged (20-24 male Fischer 344 rats were...

Renal dopamine, via activation of D1 receptors, plays a role in maintaining sodium homeostasis and BP. There exists defect renal receptor function hypertension, diabetes, aging, conditions that are associated with oxidative stress. However, the exact underlying mechanism stress-mediated impaired signaling hypertension is not known. The effect stress on was investigated healthy animals. Male Sprague-Dawley rats received tap water (vehicle) 30 mM L-buthionine sulfoximine (BSO), an oxidant,...

High salt intake produces vascular changes that contribute to the development of hypertension in salt-sensitive individuals. Because reactive oxygen species play a role pathogenesis cardiovascular diseases, we investigated whether oxidative stress contributes hypertension. Sprague-Dawley rats were divided different groups and received tap water (vehicle), 30 mmol/L l-buthionine sulfoximine ([BSO] an oxidant), high ([HS] 1% NaCl), BSO plus HS without with antioxidant tempol (1 mmol/L)...

Oxidative stress modulates angiotensin (Ang) II type 1 receptor (AT R) expression and function. Ang activates renal Na + /H exchanger 3 (NHE3) to increase sodium reabsorption, but the mechanisms are still elusive. In addition, upregulation of AT R during oxidative could promote retention lead an in blood pressure. Herein, we investigated mechanism II–mediated, R-dependent NHE3 regulation effect on signaling development hypertension. Male Sprague-Dawley rats received tap water (control) or 30...

Oxidative stress and renal inflammation play a pivotal role in the pathogenesis of hypertension. The redox-sensitive transcription factor, nuclear factor E2-related 2 (Nrf2) is master regulator phase II antioxidant enzymes that protects against oxidative inflammation. This study aimed to investigate effect Nrf2 inhibition on stress-associated hypertension dopamine 1 receptor (D1R) dysfunction mice. Male C57BL/6 J mice were treated with pro-oxidant, L-buthionine sulfoximine (BSO) (10 mmol/L...

Renal dopamine plays an important role in maintaining sodium homeostasis and blood pressure (BP) during increased intake. The present study was carried out to determine whether renal D1 receptor (D1R) dysfunction contributes increase salt sensitivity oxidative stress. Male Sprague-Dawley rats, divided into various groups, received tap water (vehicle); 1% NaCl (high [HS]); L-buthionine sulfoximine (BSO), oxidant; HS plus BSO with or without Tempol, antioxidant, for 12 days. Compared vehicle,...

Angiotensin (ANG) II via AT1 receptors (AT1Rs) maintains sodium homeostasis by regulating renal transporters including Na(+)/H(+) exchanger 3 (NHE3) in a biphasic manner. Low-ANG concentration stimulates whereas high concentrations inhibit NHE3 activity. Oxidative stress has been shown to upregulate AT1R function that could modulate the ANG II-mediated regulation. This study was designed identify signaling pathways responsible for regulation of proximal tubular and effect oxidative on this...

The association of oxidative stress with hypertension is well known. However, a causal role in unclear. Vascular angiotensin II type 1 receptor (AT1R) upregulation prominent contributor to pathogenesis hypertension. the mechanisms causing this are unknown. Oxidative an important regulator protein expression via activation transcription factors such as nuclear factor kappa B (NFκB). present study was carried out test hypothesis that contributes vascular AT1R NFκB human aortic smooth muscle...

Dopamine, via activation of renal D(1) receptors, inhibits the activities Na-K-ATPase and Na/H exchanger subsequently increases sodium excretion. Decreased dopamine production excretion are associated with type I diabetes. However, it is not known whether response to receptor altered in The present study was designed examine effect streptozotocin-induced diabetes on expression function. Streptozotocin treatment Sprague-Dawley rats caused a fourfold increase plasma levels glucose along...

Oxidative stress causes changes in angiotensin (Ang) type 1 receptor (AT1R) function, which contributes to hypertension. Ang II affects blood pressure via maintenance of sodium homeostasis by regulating renal Na + absorption through its effects on Na/K-ATPase (NKA). At low concentrations, stimulates NKA; higher concentrations inhibit the enzyme. We examined effect oxidative AT1R function involved biphasic regulation NKA. Male Sprague-Dawley rats received tap water (control) and 30 mmol/L l...

Chromium is widely used in industry but exposure to chromium compounds the workplace can result nephrotoxicity. Various nephrotoxicants affect brush border membrane (BBM) lining epithelial cells of proximal tubule, there have been no studies regarding effect potassium dichromate (K2Cr2O7), a hexavalent compound, on renal BBM. In present work, administering single intraperitoneal dose (15 mg/kg body weight) K2Cr2O7 rat BBM enzymes and inorganic phosphate (Pi) transport was studied. The...

The renal dopamine system plays an important role in sodium homeostasis and a defect D1 receptor (D1R) function is present hypertension, diabetes, aging. Our previous studies hyperinsulinemic animals cell cultures treated with insulin showed decrease D1R number defective coupling to G proteins; however, the exact mechanisms remained unknown. Therefore, we investigated insulin-mediated desensitization underlying molecular mechanism opossum kidney (OK) cells. Chronic exposure (24 h) of OK...

The dopamine D1 receptors (D1R), expressed in renal proximal tubules, participate the regulation of sodium transport. A defect coupling D1R to its G protein/effector complex tubules has been reported various conditions associated with oxidative stress. Because protein-coupled receptor kinases (GRKs) are known play an important role desensitization, we tested hypothesis that increased stress obese Zucker rats may cause GRK2 upregulation and, subsequently, dysfunction. Lean and were given...

Earlier we have reported a defective dopamine D 1 –like receptor function, which was accompanied by decrease in D1 numbers and the inability of to inhibit Na,K-ATPase Na,H-exchanger proximal tubules hyperinsulinemic obese Zucker rats. The present study designed test hypothesis that defect function is result hyperinsulinemia We experiments rats treated with rosiglitazone, as it lowers plasma insulin improving sensitivity. A group untreated lean served controls. Rosiglitazone treatment (10...

Increased renal sodium retention is considered a major risk factor contributing to hypertension associated with chronic hyperinsulinemia and obesity. However, the molecular mechanism involved not understood. The present study investigates effect of insulin treatment on AT1 receptor expression ANG II-induced stimulation Na/H exchanger (NHE) Na-K-ATPase (NKA) in opossum kidney (OK) cells, proximal tubule cell line. presence receptors OK cells was confirmed by specific binding 125I-sar-ANG II...

The role of dopamine D 1 -like receptors (DR) in the regulation renal Na + transporters, natriuresis, and blood pressure is well established. However, involvement angiotensin 1–7 (ANG 1−7)-Mas receptor balance not clear. present study aimed to investigate hypothesis that ANG can regulate homeostasis by modulating system. Sprague-Dawley rats were infused with saline alone (vehicle) or 1–7, antagonist A-779, DR agonist SKF38393, SCH23390. Infusion caused significant natriuresis diuresis...

Recently we have reported that rosiglitazone treatment of obese Zucker rats reduced plasma insulin and restored the ability dopamine to inhibit Na,K-ATPase (NKA) in renal proximal tubules. The present study was performed test hypothesis a chronic increase levels causes decrease expression D1 receptor its uncoupling from G proteins, which may account for diminished inhibitory effect on NKA rats. We conducted experiments primary tubule epithelial cells obtained Sprague-Dawley rat kidneys....