A5061415922- CRISPR and Genetic Engineering
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- SARS-CoV-2 detection and testing
- Statistical Methods and Applications
- Data Visualization and Analytics
- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Sensory Analysis and Statistical Methods
- Spectroscopy and Chemometric Analyses
- Forest ecology and management
- Gastrointestinal motility and disorders
- Nosocomial Infections in ICU
- Morphological variations and asymmetry
- Graph Theory and Algorithms
- Chromosomal and Genetic Variations
- Topological and Geometric Data Analysis
- Viral Infections and Immunology Research
- RNA and protein synthesis mechanisms
- Sepsis Diagnosis and Treatment
- Management of metastatic bone disease
- Nausea and vomiting management
- Microscopic Colitis
- Pluripotent Stem Cells Research
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
Broad Institute
2019-2022
McGovern Institute for Brain Research
2019-2022
Howard Hughes Medical Institute
2020-2022
IIT@MIT
2022
Massachusetts Institute of Technology
2019-2021
OpenBiome
2016-2020
Duke University
2020
University of North Carolina at Chapel Hill
2008-2014
Cognizant (India)
2010
Neurological Surgery
2009
CRISPR-Cas nucleases are powerful tools for manipulating nucleic acids; however, targeted insertion of DNA remains a challenge, as it requires host cell repair machinery. Here we characterize CRISPR-associated transposase from cyanobacteria
Programmable RNA editing enables reversible recoding of information for research and disease treatment. Previously, we developed a programmable adenosine-to-inosine (A-to-I) approach by fusing catalytically inactivate RNA-targeting CRISPR-Cas13 (dCas13) with the adenine deaminase domain ADAR2. Here, report cytidine-to-uridine (C-to-U) editor, referred to as Editing Specific C-to-U Exchange (RESCUE), directly evolving ADAR2 into cytidine deaminase. RESCUE doubles number mutations targetable...
Eukaryotic genomes contain domesticated genes from integrating viruses and mobile genetic elements. Among these are homologs of the capsid protein (known as Gag) long terminal repeat (LTR) retrotransposons retroviruses. We identified several mammalian Gag that form virus-like particles one LTR retrotransposon homolog, PEG10, preferentially binds facilitates vesicular secretion its own messenger RNA (mRNA). showed mRNA cargo PEG10 can be reprogrammed by flanking interest with Peg10's...
Abstract The recent outbreak of the novel coronavirus SARS-CoV-2, which causes COVID-19, can be diagnosed using RT-qPCR, but inadequate access to reagents and equipment has slowed disease detection impeded efforts mitigate viral spread. Alternative approaches based on combinations isothermal amplification CRISPR-mediated detection, such as SHERLOCK ( S pecific H igh ensitivity E nzymatic R eporter Un LOCK ing) technique, offer reduced dependence RT-qPCR equipment, previously reported methods...
We have identified the tRNAs which are incorporated into both wild-type human immunodeficiency virus type 1 strain IIIB (HIV-1IIIB) produced in COS-7 cells transfected with HIV-1 proviral DNA and mutant, noninfectious HIV-1Lai particles a genetically engineered Vero cell line. The mutant contains nucleotides 678 to 8944; i.e., long terminal repeats primer binding site absent. As analyzed by two-dimensional polyacrylamide gel electrophoresis, contain four major-abundance tRNA species, include...
Abstract RNA extraction has become a bottleneck for detection of COVID-19, in part because reagent shortages. We present here rapid protocol that circumvents the need is compatible with RT-qPCR-based methods.
Hutchinson-Gilford progeria syndrome (HGPS) is a rare disorder caused by point mutation in the Lamin A gene that produces protein progerin. Progerin toxicity leads to accelerated aging and death from cardiovascular disease. To elucidate effects of progerin on endothelial cells, we prepared tissue-engineered blood vessels (viTEBVs) using induced pluripotent stem cell-derived smooth muscle cells (viSMCs) (viECs) HGPS patients. viECs aligned with flow but exhibited reduced flow-responsive...
Rapid and extensive testing of large parts the population specific subgroups is crucial for proper management severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections decision-making in times a pandemic outbreak. However, point-of-care (POC) places such as emergency units, outpatient clinics, airport security points or entrance any public building major challenge. The need thermal cycling nucleic acid isolation hampers use standard PCR-based methods this purpose.To avoid these...
In 1999, the Society of Critical Care Medicine formally recognized that pharmacists were essential for provision high quality care to critically ill population. This study is a brief quantitative analysis benefit provided by clinical pharmacist in multidisciplinary neurosurgical setting.Patients admitted service 2 years before and after implementation dedicated pharmacy services retrospectively reviewed. The was responsible monitoring evaluating all adult patients on rounding with team 6...
Brain metastases are an increasingly important determinant of survival and quality life in patients with cancer. Current approaches to the management brain driven by prognostic factors, including Karnofsky Performance Status, tumor histology, number metastases, patient age, status systemic disease. Most treated radiosurgery, computer-assisted surgery, or whole radiation therapy. Remarkable advances neuronavigation have made neurosurgical removal safer, even eloquent areas brain....
Point-of-care (POC) nucleic acid detection technologies are poised to aid gold-standard in controlling the COVID-19 pandemic, yet shortcomings capability perform critically needed complex detection-such as multiplexed for viral variant surveillance-may limit their widespread adoption. Herein, we developed a robust clustered regularly interspaced short palindromic repeats (CRISPR)-based using LwaCas13a and PsmCas13b simultaneously diagnose severe acute respiratory syndrome coronavirus 2...
Fecal microbiota transplantation (FMT) is a recommended therapy to prevent recurrent Clostridioides difficile infection (CDI).1,2 Stool banks have emerged enable safe widespread access by provid...
Looking for empty parking spaces in congested can be painstaking and time consuming. The average spent bays cruising vacant approximately varies from 3.5-12 minutes. These cars also add to the traffic pollution inside bay. present management system urban cities of growing economies like India lacks efficiency, often leaving drivers frustrated. We are engaged developing an automated employing Wireless Sensor Network (WSN) technology. detect presence and/or absence a vehicle respective...
Background: Continuous insulin infusions have become a standard of care in many intensive units (ICUs); however, methods for transitioning patients from continuous to other therapies not been extensively evaluated. Objective: To determine the most effective method ICU subcutaneous therapy. Methods: A searchable pharmacy database at University North Carolina Hospitals was used retrospectively identify adults admitted neurosurgery and prescribed infusion between May 2007 February 2008, All...
Abstract Background Rapid and extensive testing of large parts the population specific subgroups is crucial for proper management severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections decision-making in times a pandemic outbreak. However, point-of-care (POC) places such as emergency units, outpatient clinics, airport security points or entrance any public building major challenge. The need thermal cycling nucleic acid isolation hampers use standard PCR-based methods this...
Developments in science and technology over the last two decades has motivated study of complex data objects. In this article, we consider topological properties a population tree-structured Our interest centers on modeling relationship between response other covariates. For objects, poses serious challenges since most regression methods rely linear operations Euclidean space. We generalize notion nonparametric to case variable. addition, develop fast algorithm give its theoretical...
This study introduces a new method of visualizing complex tree structured objects. The usefulness this is illustrated in the context detecting unexpected features data set very large trees. major contribution novel two-dimensional graphical representation each tree, with covariate coded by color. motivating contains three dimensional representations brain artery systems 105 subjects. Due to inaccuracies inherent medical imaging techniques, issues reconstruction algo- rithms and...
Tracheal aspirate Gram's stains are used to guide antibiotic selection in empiric pneumonia treatment the surgical intensive care unit (SICU). We questioned whether predict organism cultured.A retrospective review of prospectively collected data.Gram's correlated with cultured 284 543 (52%) SICU cultures and 226 403 (56%) trauma (TICU) cultures. Gram-negative rod (GNR) yielded GNR organisms 182 205 (89%) 160 176 (91%) TICU Gram-positive coccus (GPC) GPC 75 228 (33%) 52 149 (35%)...
Introduction: Fecal microbiota transplantation (FMT) is an effective therapy for recurrent Clostridium difficile infection (CDI) and stool banks have enabled widespread adoption by providing pre-processed samples that can be stored locally until clinically required. It unknown whether variation in processing time or storage duration of banked material may compromise bacterial viability reduce clinical effectiveness. In-vitro work suggests changes are modest, however, given the mechanism...
Abstract Point-of-care (POC) nucleic acid detection technologies are poised to aid gold-standard in controlling the COVID-19 pandemic, yet shortcomings capability perform critically needed complex detection—such as multiplexed for viral variant surveillance—may limit their widespread adoption. Herein, we developed a robust CRISPR-based using LwaCas13a and PsmCas13b simultaneously diagnose SARS-CoV-2 infection pinpoint causative of concern (VOC)— including globally dominant VOCs Delta...