A5024058809- Renal Diseases and Glomerulopathies
- Blood groups and transfusion
- Reproductive System and Pregnancy
- Monoclonal and Polyclonal Antibodies Research
- Chronic Kidney Disease and Diabetes
- Renal Transplantation Outcomes and Treatments
- Metabolism, Diabetes, and Cancer
- T-cell and B-cell Immunology
- Cell Adhesion Molecules Research
- Pregnancy and Medication Impact
- Dialysis and Renal Disease Management
- Nitric Oxide and Endothelin Effects
- Immunodeficiency and Autoimmune Disorders
- Pancreatic function and diabetes
- Renal and Vascular Pathologies
- Complement system in diseases
- Platelet Disorders and Treatments
- Pregnancy and preeclampsia studies
- Renal and related cancers
- Hemoglobinopathies and Related Disorders
- NF-κB Signaling Pathways
- Organ Transplantation Techniques and Outcomes
- Xenotransplantation and immune response
- Proteoglycans and glycosaminoglycans research
- Renin-Angiotensin System Studies
The University of Melbourne
2014-2024
Institute for Breathing and Sleep
2014-2024
Austin Health
2015-2024
Imperial College Healthcare NHS Trust
2016-2023
St Mary's Hospital
2023
St. Mary's Hospital
2023
University Hospital Limerick
2018-2020
Imperial College London
2020
St Vincent's Hospital
1993-2019
Kidney Research UK
2018
The AMP‐activated protein kinase (AMPK) in rat skeletal and cardiac muscle is activated by vigorous exercise ischaemic stress. Under these conditions AMPK phosphorylates inhibits acetyl‐coenzyme A carboxylase causing increased oxidation of fatty acids. Here we show that co‐immunoprecipitates with endothelial NO synthase (eNOS) Ser‐1177 the presence Ca 2+ ‐calmodulin (CaM) to activate eNOS both vitro during ischaemia hearts. In absence ‐calmodulin, also at Thr‐495 CaM‐binding sequence,...
The structural basis of normoalbuminuric renal insufficiency in patients with type 2 diabetes remains to be elucidated. We compared biopsy findings and estimated glomerular filtration rate (eGFR) measured GFR <60 mL/min/1.73 m2, associated either normo-, micro-, or macroalbuminuria.In normo- (n = 8) microalbuminuria 6), biopsies were performed according a research protocol. In macroalbuminuria 17), clinical indication. Findings categorized the Fioretto classification: category 1 (C1),...
OBJECTIVE—Anemia is common in diabetes, potentially contributing to the pathogenesis of diabetes complications. This study aims establish prevalence and independent predictors anemia a cross-sectional survey 820 patients with long-term follow-up single clinic. RESEARCH DESIGN AND METHODS—A full blood count was obtained addition routine urine test results for all over 2-year period encompass patterns review. Predictors most recent Hb concentration were identified using multiple logistic...
NADPH oxidase–derived excessive production of reactive oxygen species (ROS) in the kidney plays a key role mediating renal injury diabetes. Pathological changes diabetes include mesangial expansion and accumulation extracellular matrix (ECM) leading to glomerulosclerosis. There is paucity data about Nox5 isoform oxidase animal models diabetic nephropathy since absent mouse genome. Thus, we examined human cells an inducible transgenic exposed streptozotocin-induced In biopsies, was identified...
Background Expression of genes regulating fatty acid metabolism is reduced in tubular epithelial cells from kidneys with tubulointerstitial fibrosis (TIF), thus decreasing the energy produced by oxidation (FAO). Acetyl-CoA carboxylase (ACC), a target for energy-sensing AMP-activating protein kinase (AMPK), major controller rate FAO within cells. Metformin has well described antifibrotic effect, and increases phosphorylation ACC AMPK, thereby increasing FAO. Methods We evaluated cell mouse...
Graft-derived cell-free DNA (donor-derived DNA) is an emerging marker of kidney allograft injury. Studies examining the clinical validity this biomarker have previously used graft fraction, or proportion total that graft-derived. The present study evaluated diagnostic absolute measurements graft-derived DNA, as well calculated for diagnosis dysfunction. Plasma and fraction were correlated with biopsy individual Banff scores. Sixty-one samples included in analysis. For antibody mediated...
The sequelae of diabetes include microvascular complications such as diabetic kidney disease (DKD), which involves glucose-mediated renal injury associated with a disruption in mitochondrial metabolic agility, inflammation, and fibrosis. We explored the role innate immune complement component C5a, potent mediator pathogenesis DKD clinical experimental diabetes. Marked systemic elevation C5a activity was demonstrated patients diabetes; conventional renoprotective agents did not...
Background and Purpose— Fibrinogen is an independent risk factor for coronary events in population-based studies patients with heart disease, but there uncertainty about prediction of stroke, particularly secondary prevention. Methods— We studied unpublished data from 3 prospective recent transient ischemic attack (TIA) or minor stroke: the United Kingdom TIA Aspirin (UK-TIA) trial (n=1860); Dutch (n=2960); Oxford Study (n=293). By separate pooled analysis, we used Cox models to determine...
Weight loss is common in Alzheimer's disease (AD) and predictive of mortality. Leptin, an adipocyte-derived peptide hormone implicated the regulation satiety energy expenditure. It acts on hypothalamus to suppress appetite increase We undertook this study determine if inappropriately elevated leptin levels play a role AD-associated weight loss. Serum 8 patients each following groups were determined: (1) AD, body mass index (BMI) >25; (2) BMI <20; (3) non-Alzheimer's (vascular) dementia...
Objective: To determine whether eating Lactobacillus rhamnosus GG (LGG) in the form of commercially available yoghurt improves clearance vancomycin-resistant enterococci (VRE). Design: Double-blind, randomised, placebo-controlled trial. Setting: Renal ward Austin Health, a tertiary hospital, Feb–Oct 2005. Participants: 27 VRE-positive patients, 14 receiving active treatment and 13 controls. Interventions: Subjects were randomly assigned to either group (receiving 100 g daily containing LGG...
OBJECTIVE—To investigate the role of intrarenal vascular disease in pathogenesis nonalbuminuric renal insufficiency type 2 diabetes. RESEARCH DESIGN AND METHODS—We studied 325 unselected clinic patients who had sufficient clinical and biochemical information to calculate an estimated glomerular filtration rate (eGFR) using Modified Diet Renal Disease six-variable formula, at least two estimations urinary albumin excretion rates (AER), a duplex scan estimate resistance index interlobar...
Mutations in SCARB2 were recently described as causing action myoclonus renal failure syndrome (AMRF). We hypothesized that mutations might account for unsolved cases of progressive epilepsy (PME) without impairment, especially those resembling Unverricht-Lundborg disease (ULD). Additionally, we searched the PRICKLE1 gene, newly recognized a cause PME mimicking ULD.We reviewed referred diagnosis over two decades which molecular had not been reached. Patients classified according to age...
Summary Background and objectives Retinal abnormalities are common in inherited acquired renal disease. This study determined the prevalence of retinal chronic kidney disease (CKD) stages 3 to 5. Design, setting, participants, & measurements One hundred fifty patients with CKD 5 150 age- gender-matched hospital 1 2 underwent bilateral photography. These images were reviewed for incidental abnormalities, microvascular (Wong Mitchell classification) diabetic retinopathy (Airlie House...
Excessive production of renal reactive oxygen species (ROS) plays a major role in diabetic kidney disease (DKD). Here, we provide key findings demonstrating the predominant pathological pro-oxidant enzyme NADPH oxidase 5 (NOX5) DKD, independent previously characterized NOX4 pathway. In patients with diabetes, found increased expression NOX5 association enhanced ROS formation and upregulation ROS-sensitive factors early growth response 1 (EGR-1), protein kinase C-α (PKC-α), metabolic gene...