ABSTRACT We report the development of a multiplex PCR protocol for diagnosis staphylococcal infection. The was designed to (i) detect any species exclusion other bacterial pathogens (based on primers corresponding Staphylococcus -specific regions 16S rRNA genes), (ii) distinguish between S. aureus and coagulase-negative staphylococci (CNS) amplification clfA gene), (iii) provide an indication likelihood that present in specimen are resistant oxacillin mecA gene). expected fragments were...

Objective: To investigate maternal and neonatal outcomes after antioxidant supplementation relatively early in pregnancy (8 to 12 weeks) for pregnant women with low status. Methods: A randomized, double-blind, placebo-controlled trial of daily was performed on screening positive status at 8 weeks gestation. Low defined as a superoxidedismutase (SOD) level below 1102 U/g Hb or 164 U/mL. The group received the following antioxidants daily: vitamins (1000 IU), B6 (2.2 mg), B12 μg), C (200 E...

Abstract Objective To evaluate the direct alterations in mRNA expression among chorionic villous samples from 11 weeks' pregnant women who would develop preeclampsia (PE) later pregnancy. Method Case‐control study encompassing five destined to PE [cases matched 1:5 for gestational age (GA) with 25 controls]. We quantified on tissue sampling (CVS) of normal and patients. then assessed expressions vascular endothelial growth factor (VEGFA), VEGFA receptor 1 (Flt‐1), endoglin (Eng), placental...

Background . Preterm birth is still a global burden particularly in Indonesia. The suboptimal concentration of certain micronutrients and heavy metals hypothesized to play role the mechanism preterm birth. Objective This study aimed analyze concentrations between subjects with term Design A cross-sectional was conducted during January–June 2017 Cipto Mangunkusumo Hospital Budi Kemuliaan Hospital, Jakarta, Subjects were divided into groups. measured outcomes maternal serum, placental, blood...

The lack of standardized clinical practice impeding the optimal management iron deficiency (ID) and anemia (IDA) in women is a global concern, particularly Asia-Pacific region. aim this study was to determine best practices through Delphi consensus process. In Round 1, panelists were asked rate their level agreement with 99 statements across four domains: identification, diagnosis assessment, prevention, treatment ID/IDA women. 2, reappraised ratings view collective feedback responses 1....

To compare mRNA concentrations of corticotrophin-releasing hormone (CRH), placenta specific-1 (PLAC1), and selectin-P in preeclamptic normal pregnancies.Peripheral blood samples were obtained from 43 pregnant women with preeclampsia 41 control subjects. Plasma was harvested RNA extracted. analyzed using reverse transcription polymerase chain reaction (PCR) assay. Median CRH, PLAC1, plasma compared, to assess possible differences distribution. Data also stratified compared according clinical...

BACKGROUND: Oxidative stress and trace elements in erythrocytes are linked to impaired nitric oxide that can lead endothelial dysfunction preeclampsia patients. The morphology of could also be affected by oxidative elements. While the relationships between erythrocyte index, superoxide dismutase (SOD) activity, have been well established, less attention has given their role disease progression. This study was performed examine element profile women with preeclampsia, comparing it controls....

Detection of placental mRNA in maternal plasma has been reported high-risk pregnancies. We attempted to investigate the concentrations plasminogen activator inhibitor-1 (PAI-1) and tissue-type (tPA) preeclampsia.Peripheral blood samples were obtained from healthy pregnant women before after delivery also with or without preeclampsia. Plasma was isolated these samples, RNA extracted. PAI-1 tPA then measured by use reverse transcription PCR assays. The converted into multiples median (MoM)...

Abstract Objective To assess the alterations of mRNA expressions associated with oxidative stress in cellular component blood from pregnant women pre‐eclampsia. Methods Peripheral samples were obtained and without Cellular RNA was subjected to a reverse transcription polymerase chain reaction (PCR) assay order examine distribution among pre‐eclampsia ( n = 24) control subjects during 35–41 weeks gestation. The data analyzed by non‐parametric statistics. Results Significant differences...

Background Preeclampsia has become the world's major maternal health problem putting a huge burden on mothers, newborns and also systems. The pathogenesis of preeclampsia seems to include events in very early pregnancy affecting differentiation placental villous trophoblast. arising changes cell death spectrum from apoptosis via increased autophagy aponecrosis necrosis turn induce systemic inflammation mother. Methods Placental tissue samples serum 40 pregnant women were collected normal...

A panel of cellular mRNA markers was used to predict the occurrence pre-eclampsia in pregnant women at 15-20 weeks gestation.Prospective cohort study.The Department Obstetrics and Gynaecology, University Indonesia, Cipto Mangunkusumo National Hospital, Indonesia.Peripheral blood samples from asymptomatic women.Among 660 women, 62 developed later gestation (pre-eclampsia group) each case matched with five controls. Therefore, RNA expression levels component maternal were compared those 310...

Abstract Aim: The aim of this study was to investigate the benefit antioxidant supplementation in a cohort women with low status and determine changes cell‐free mRNA. Material Methods: This randomized, placebo‐controlled trial 8–12 weeks' pregnant who had treated either antioxidants or control diets daily until 2 postpartum. primary end‐point risk pre‐eclampsia secondary angiogenic anti‐oxidant mRNA markers related outcome (ClinicalTrial.gov, number NCT01232205). Results: There were 110...

In Brief OBJECTIVE: To perform gene expression profiling and real-time quantitative reverse-transcription polymerase chain reaction (PCR) analysis to identify biomarkers of preeclampsia in cellular messenger RNA (mRNA) from maternal blood. METHODS: We performed a microarray with five blood samples women matched control subjects. Up-regulated was further analyzed through PCR 28 consecutive affected 29 controls. RESULTS: Both pregnancy-specific β1 glycoprotein trophoblast were selected based...

Preeclampsia and intra-uterine growth restriction (IUGR) are major health problems during pregnancy affecting both mother child. Defective placental development failure of trophoblast differentiation important aspects in the pathogenesis syndromes. Recent studies have shown that autophagy is involved survival capacity. As vitamin D has a central role many cellular processes, we studied relation those processes preeclampsia IUGR.Serum samples from four groups cases; normal term, IUGR,...

Abstract Long-Chain Polyunsaturated Fatty Acid (LCPUFA) is essential throughout pregnancy, since deficiency of LPUFA may linked to obstetrical complications. This study aimed investigate LCPUFA status in severe preeclampsia and preterm birth. A cross sectional was conducted 104 pregnant women, which divided into normal birth groups. Serum percentage concentration total LCPUFA, omega-3, alpha-linolenic acid (ALA), eicosapentaenoic (EPA), docosahexaenoic (DHA), omega-6, linoleic (LA),...

Preeclampsia has been one of the leading causes maternal death in Indonesia. It is postulated that its relationship with oxidative stress may be underlying pathology disease. Nutrients and amino acids have suggested as a scavenger for oxygen-free radicals. No previous study regarding acid status preeclampsia conducted women Indonesia.This was cross-sectional total 64 pregnant women, 30 normal pregnancy 34 severe preeclampsia. Data were obtained Cipto Mangunkusumo National Referral Hospital...

Background: Preeclampsia has great implication on adverse neonatal outcome. Appearance, pulse, grimace, activity, respiration (APGAR) score at 1 or 5 minutes is one of the indicators physiologic maturity infant. Therefore, aim this study was to know correlation APGAR in preeclamptic deliveries with its risk factors. Methods: This a retrospective cohort. Data were collected from January December 2013 including all women singleton live pregnancies who delivered their babies Cipto Mangunkusumo...

Introduction. Senescent cells have been demonstrated to release High Mobility Group Box 1 (HMGB1) which induces labor through an inflammatory pathway. This research is aimed at demonstrating whether telomere shortening, proinflammatory HMGB1, and oxidative damage marker 8-OHdG play a role in the placenta of preterm birth comparison term birth. Method. A cross-sectional study on 67 full thickness obtained from mothers with Mothers clinical signs infection ( <math...

Background: Maternal nutrition before and during pregnancy is important for a healthy outcome. According to National Basic Health Research (Riskesdas) 2013, 24.2% of pregnant women are at risk chronic malnutrition 37.1% them suffer from anemia. The aim this study was obtain information about the nutrient intake serum micronutrient status in first trimester Jakarta.Methods: A descriptive conducted towards 234 with gestational age no more than 14 weeks. data obtained conversion frequency food...