Three-dimensional (3D) digital brain atlases and high-throughput brain-wide imaging techniques generate large multidimensional datasets that can be registered to a common reference frame. Generating insights from such depends critically on visualization interactive data exploration, but this challenging task. Currently available software is dedicated single atlases, model species or types, generating 3D renderings merge anatomically diverse sources requires extensive development programming...

Abstract Animals generate neural representations of their heading direction. Notably, in insects, direction is topographically represented by the activity neurons central complex. Although head cells have been found vertebrates, connectivity that endows them with properties unknown. Using volumetric lightsheet imaging, we find a topographical representation neuronal network zebrafish anterior hindbrain, where sinusoidal bump rotates following directional swims fish and otherwise stable over...

We present Stytra, a flexible, open-source software package, written in Python and designed to cover all the general requirements involved larval zebrafish behavioral experiments. It provides timed stimulus presentation, interfacing with external devices simultaneous real-time tracking of parameters such as position, orientation, tail eye motion both freely-swimming head-restrained preparations. Stytra logs recorded quantities, metadata, code version standardized formats allow full...

Abstract Many oligodendrocyte precursor cells (OPCs) do not differentiate to form myelin, suggesting additional roles of this cell population. The zebrafish optic tectum contains OPCs in regions devoid myelin. Elimination these impaired precise control retinal ganglion axon arbor size during formation and maturation retinotectal connectivity degraded functional processing visual stimuli. Therefore, fine-tune neural circuits independently their canonical role make

Neuroscientists routinely perform experiments aimed at recording or manipulating neural activity, uncovering physiological processes underlying brain function elucidating aspects of anatomy.Understanding how the generates behaviour ultimately depends on merging results these into a unified picture anatomy and function.Brain atlases are crucial in this endeavour: by outlining organization regions they provide reference upon which our understanding can be anchored.More recently, digital...

Animals must adapt their behavior to survive in a changing environment. Behavioral adaptations can be evoked by two mechanisms: feedback control and internal-model-based control. Feedback controllers maintain the sensory state of animal at desired level under different environmental conditions. In contrast, internal models learn relationship between motor output its consequences used recalibrate behaviors. Here, we present multiple unpredictable perturbations visual larval zebrafish...

Impairments of the dialogue between excitation and inhibition is commonly associated to neuropsychiatric disorders like autism, bipolar epilepsy. Moderate levels hyperexcitability can lead mild alterations EEG are often with cognitive deficits even in absence overt seizures. Indeed, various testing paradigms have shown degraded performances presence acute or chronic non-ictal epileptiform activity. Evidences from both animal models clinics suggest that anomalous activity cause by transiently...

Sampling behaviors have sensory consequences that can hinder perceptual stability. In olfaction, sniffing affects early odor encoding, mimicking a sudden change in concentration. We examined how the inhalation speed representation of concentration main olfactory cortex. Neurons combine input with global top-down signal preceding sniff and mechanosensory feedback generated by air passage through nose during inhalation. Still, population is remarkably invariant. This because responses are...

The cerebellum is a conserved structure of the vertebrate brain involved in timing and calibration movements. Its function supported by convergence fibers from granule cells (GCs) inferior olive neurons (IONs) onto Purkinje (PCs). Theories cerebellar postulate that IONs convey error signals to PCs that, paired with contextual information provided GCs, can instruct motor learning. Here, we use larval zebrafish investigate (1) how sensory representations same stimulus vary across GCs (2) PC...

Abstract The recent development of high-resolution three-dimensional (3D) digital brain atlases and high-throughput wide imaging techniques has fueled the generation large datasets that can be registered to a common reference frame. This registration facilitates integrating data from different sources resolutions assemble rich multidimensional datasets. Generating insights these new types depends critically on ability easily visualize explore in an interactive manner. is, however,...

Abstract Animals can use different strategies to navigate. They may guide their movements by relying on external cues in environment or, alternatively, using an internal cognitive map of the space around them and position within it. An essential part this representation are heading cells, neurons whose activity depends direction animal. Although those cells have been found vertebrates, full network has never observed there is very little mechanistic understanding how these acquire response...

Sensory information is fundamental for navigation. Visual motion used by animals to estimate their traveling distance and direction, visual landmarks allow tether location orientation environment. How such signals are integrated in the vertebrate brain poorly understood. Here we investigate representation of directional whole field landmark position a circuit larval zebrafish consisting habenula, interpeduncular nucleus (IPN) anterior hindbrain (aHB). This has been recently implicated...

Abstract Epileptiform activity is associated with impairment of brain function even in absence seizures, as demonstrated by failures various testing paradigm presence hypersynchronous interictal spikes (ISs). Clinical evidence suggests that cognitive deficits might be directly caused the anomalous rather than its underlying etiology. Indeed, we seek to understand whether ISs interfere neuronal processing connected areas not participating an acute model epilepsy. Here cause focal visual...

Abstract The cerebellum is a highly conserved structure of the vertebrate central nervous system that plays role in timing and calibration motor sequences. Its function supported by convergence fibers from granule cells (GCs) inferior olive neurons (IONs) onto Purkinje (PCs). Theories cerebellar postulate IONs convey error signals to PCs that, paired with contextual information provided GCs, can be used as teaching signal guide learning. Here, we use larval zebrafish investigate (i) how...

Abstract Many oligodendrocyte precursor cells (OPCs) do not differentiate to form myelin, suggesting additional roles of this cell population. The zebrafish optic tectum contains OPCs in regions devoid myelin. Elimination these impaired precise control retinal ganglion axon arbor size during formation and maturation retinotectal connectivity, consequence visual acuity. Therefore, fine-tune neural circuit structure function independently their canonical role make

Abstract Animals must adapt their behavior to survive in a changing environment. Behavioral adaptations can be evoked by two mechanisms: feedback control and internal-model-based control. Feedback controllers maintain the sensory state of animal at desired level under different environmental conditions. In turn, internal models learn relationship between resulting consequences order modify when this changes. Here, we present multiple perturbations visual larval zebrafish performing optomotor...

We present Stytra, a flexible, open-source software package, written in Python, that we designed to cover all the general requirements involved larval zebrafish behavioral experiments. It allows timed stimulus presentation, interfacing with external devices and simultaneous real-time tracking of position, tail eye motion both freely-swimming head-restrained preparations. logs standardized formats recorded quantities, metadata, code version allow full provenance tracking, from data...

Abstract The habenulo-interpeduncular pathway is a highly conserved neural circuit across vertebrates, but the anatomical and functional architecture of interpeduncular nucleus (IPN) remains poorly understood. Here, we use combination immunohistochemistry, volumetric electron microscopy (EM), two-photon imaging to provide first detailed characterization internal organization IPN in larval zebrafish. We show that receives extensive projections from tegmentum, reveal strict segregation between...