A5066546365- Alzheimer's disease research and treatments
- Neuroscience and Neuropharmacology Research
- Computational Drug Discovery Methods
- Spectroscopy Techniques in Biomedical and Chemical Research
- Mitochondrial Function and Pathology
- Cellular transport and secretion
- Metabolomics and Mass Spectrometry Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Nuclear Receptors and Signaling
- Medicinal Plants and Neuroprotection
- Neurogenesis and neuroplasticity mechanisms
- Parkinson's Disease Mechanisms and Treatments
- Calcium signaling and nucleotide metabolism
- Receptor Mechanisms and Signaling
- Mass Spectrometry Techniques and Applications
- Lipid Membrane Structure and Behavior
- Spectroscopy and Chemometric Analyses
- Essential Oils and Antimicrobial Activity
Lund University
2020-2024
Lund Science (Sweden)
2023
Infrared spectroscopic imaging is widely used for the visualization of biomolecule structures, and techniques such as optical photothermal infrared (OPTIR) microspectroscopy can achieve <500 nm spatial resolution. However, these approaches lack specificity particular cell types components thus cannot be a stand-alone technique to assess their properties. Here, we have developed novel tool, fluorescently guided microspectroscopy, that simultaneously exploits epifluorescence OPTIR perform IR...
Synaptic changes and neuronal network dysfunction are among the earliest in Alzheimer’s disease (AD). Apolipoprotein E4 (ApoE4), major genetic risk factor AD, has been shown to be present at synapses induce hyperexcitability mouse knock-in brain regions vulnerable AD. ApoE is mainly generated by astrocytes, however, neurons can also produce under stress conditions such as aging. The potential synaptic function(s) of whether cellular source might affect excitability remain poorly understood....
Alzheimer’s disease (AD) is increasingly seen as a of synapses and diverse evidence has implicated the amyloid-β peptide (Aβ) in synapse damage. The molecular cellular mechanism(s) by which Aβ and/or its precursor protein, amyloid protein (APP) can affect remains unclear. Interestingly, early hyperexcitability been described human AD mouse models AD, precedes later hypoactivity. Here we show that neurons culture with either elevated levels or APP mutated to prevent generation both induce...
Apolipoprotein E (APOE), one of the primary lipoproteins in brain has three isoforms humans, APOE2, APOE3, and APOE4. APOE4 is most well-established risk factor increasing predisposition for Alzheimer's disease (AD). The presence allele alone shown to cause synaptic defects neurons recent studies have identified multiple pathways directly influenced by However, mechanisms underlying APOE4-induced dysfunction remain elusive. Here, we report that acute exposure cortical or synaptoneurosomes...
Label-free chemical imaging of living and functioning systems is the holy grail biochemical research. However, existing techniques often require extensive sample preparation to remove interfering molecules such as water, rendering many molecular unsuitable for in situ structural studies. Here, we examined freshly extracted tissue biopsies small vertebrates at submicrometer resolution using optical photothermal infrared (O-PTIR) microspectroscopy demonstrated following major advances: (1)...
Apolipoprotein E4 (ApoE4) is the most important genetic risk factor for Alzheimer's disease (AD). Among earliest changes in AD endosomal enlargement neurons, which was reported as enhanced ApoE4 carriers. ApoE thought to be internalized into endosomes of whereas β-amyloid (Aβ) accumulates within neuronal early AD. However, it remains unknown whether and Aβ intersect intracellularly. We show that astrocytic localizes mostly lysosomes neuroblastoma cells astrocytes, preferentially...
Abstract Label-free chemical and structural imaging of complex living tissue biological systems is the holy grail biomedical research clinical diagnostics. The current analysis techniques are time-consuming and/or require extensive sample preparation, often due to presence interfering molecules such as water, making them unsuitable for systems. Here, we demonstrate a proof-of-principle study using label-free optical photothermal mid-infrared microspectroscopy (O-PTIR) fast, direct...
Abstract Background Apolipoprotein E4 (ApoE4) is the main genetic risk factor for Alzheimer’s disease (AD). Previous research supports that ApoE4 increases amyloid‐β (Aβ) aggregation and plaque deposition. However, it remains poorly understood how affects of Aβ prior to seeding on a cellular level. The aim this project use high‐resolution techniques study intraneuronal accumulation at synaptic subcellular level investigate different ApoE isoforms influence this. Method in cultured primary...
Abstract Background APOE genotype is known as the most important genetic risk factor for developing AD. Understanding cellular differences between ApoE isoforms will be crucial understanding fundamental pathways that are disrupted in AD and potential can therapeutically targeted. Accumulating evidence suggesting influence endosomal system, one of earliest sites affected However, our how affect endolysosomal pathway still limited. We aim to understand pathway, but also dynamics system context...
Abstract Apolipoprotein E (APOE), one of the primary lipoproteins in brain has three isoforms humans – APOE2, APOE3, and APOE4. APOE4 is most well-established risk factor increasing pre-disposition for Alzheimer’s disease. The presence allele alone shown to cause synaptic defects neurons recent studies have identified multiple pathways directly influenced by However, mechanisms underlying induced dysfunction remain elusive. Here, we report that acute exposure cortical leads a significant...
Abstract Background ApoE4, the most important genetic risk factor for Alzheimer’s disease (AD), is mainly produced by astrocytes, to some extent microglia, and under stress also neurons. Under healthy conditions ApoE transports lipids from astrocytes neurons, where it binds receptors internalized. ApoE4 was reported impair normal receptor recycling, modulates synaptic associates with enlarged endosomes in AD. target replacement (TR) mice absence of AD mutations show neuronal hyperactivity....
Abstract The proper function of the nervous system is dependent on appropriate timing neuronal firing. Synapses continually undergo rapid activity-dependent modifications that require feedback mechanisms to maintain network activity within a window in which communication energy efficient and meaningful. Homeostatic synaptic plasticity (HSP) homeostatic intrinsic (HIP) are such negative mechanisms. Accumulating evidence implicates Alzheimer’s disease (AD)-related amyloid precursor protein...
Summary Apolipoprotein E4 (ApoE4) is the most important genetic risk factor for Alzheimer’s disease (AD). Among earliest changes in AD endosomal enlargement neurons, which was reported as enhanced ApoE4 carriers. ApoE thought to be internalized into endosomes of while β-amyloid (Aβ) accumulates within neuronal early AD. However, it remains unknown whether and Aβ intersect intracellularly. We show that astrocytic localizes mostly lysosomes neuroblastoma cells astrocytes, neurons...
The presence of an apolipoprotein E4 (ApoE4) genotype is the major genetic risk factor for Alzheimer's disease (AD). Astrocytes are main source ApoE in brain, however can also be produced by neurons and microglia. plays a role many cell types processes related to AD, however, it remains unclear which mechanism(s) cellular most critical AD. One earliest changes AD early such as endosomal synaptic alterations. ApoE4 has been associated with impaired trafficking dysregulated plasticity....