Canine distemper virus (CDV) is a major pathogen of dogs and represents serious threat to both unvaccinated vaccinated animals. This study surveyed with or without clinical signs related canine from different regions Brazil 2008 2012. A total 155 out 386 animals were found be CDV positive by RT-PCR; 37 (23.8%) asymptomatic at the time sampling, 90 (58%) displayed suggestive distemper. Nineteen (12.2%) had record complete vaccination, 15 (9.6%) an incomplete vaccination protocol, 76 (49%) no...

Escherichia coli is a commensal bacterium of the bird's intestinal tract, but it can invade different tissues resulting in systemic symptoms (colibacillosis). This disease occurs only when E. infecting strain presents virulence factors (encoded by specific genes) that enable adhesion and proliferation host organism. Thus, important to differentiate pathogenic (APEC, avian coli) non-pathogenic or fecal (AFEC, isolates. Previous studies analyzed occurrence strains isolated from birds with...

AbstractSalmonella enterica serovar Gallinarum biovar is a pathogenic bacterium that causes fowl typhoid (FT), affecting chicken flocks worldwide. This study aimed to evaluate the emergence, dissemination and genomic profile of S. lineages from Brazil. Twelve whole-genomes sequences (WGS) different strains isolated Brazilian poultry farms (2014 2018) were obtained used construct dataset with other 31 previously published data (five more Brazil). phylogenetic diversity, temporal evolution...

Colibacillosis is a chicken disease caused by avian pathogenic Escherichia coli (APEC). Pathogenicity in birds determined the occurrence of bacterial genes encoding virulence factors APEC strains. Furthermore, and other infections commercial poultry farms have been treated with intensive use antimicrobials for decades. Currently, many strains are no longer susceptible to frequently used antibiotics due increasing antimicrobial resistance (AMR) associated acquisition mutation specific genes....

Canine parvovirus type 2 (CPV-2) is a relevant pathogen for dogs and causes severe disease in carnivore species. CPV-2 reached pandemic proportions after the 1970s with worldwide dissemination, generating antigenic genetic variants (CPV-2a, CPV-2b, CPV-2c) different pathobiology comparison original CPV-2. The present study aimed to assess current global molecular phylogeny analyze diversity temporal spreading of from Brazil. A total 284 whole-genome sequences (WGS) 684 VP2 complete genes...

The prediction of outcome is one the major problems associated with traumatic brain injury. Recently, investigations have been performed on potential use circulating cell-free DNA in plasma for clinical diagnosis and prognosis a variety conditions. In this study, we investigated concentrations after severe injury (TBI) its correlation primary outcome. We studied 41 male victims TBI, isolated TBI or exracranial injuries. Control samples were obtained from 13 healthy volunteers. Plasma was...

Avian infectious bronchitis is a highly contagious viral disease with economic effects on poultry agribusiness. The presents multi-systemic clinical signs (respiratory, renal, enteric, and reproductive) caused by one coronavirus (infectious virus, IBV). Infectious virus classified into different serotypes genotypes (vaccine strains field variants). This study aimed to evaluate the occurrence of IBV in commercial flocks from 3 important producing regions Brazil determine tropism main...

Human papillomavirus (HPV) infection is common in sexually active women and viral persistence may cause intraepithelial lesions eventually progress to cervical cancer (CC). The present study aimed investigate epidemiological factors related HPV evaluate CC precursor frequencies from a city the countryside of South Brazil. Three hundred were recruited primary public health care clinic. patients interviewed underwent sampling with brushes for HPV-DNA detection/typing by PCR-based assay...

Infectious bronchitis virus (IBV) is the agent of a highly contagious disease that affects domestic fowl (Gallus gallus). Recent reports showed high prevalence one main IBV genotype (Brazil or BR-I) with low genetic diversity in commercial poultry flocks from Brazil. This research analyzed positive different rearing regions to verify S1 gene variability and geographic distribution variant strains recent years (2010 2011). Samples IBV-positive were obtained 60 farms. Forty-nine partial...

Hepatitis B virus (HBV) infection is a major public health problem in Brazil. Several risk factors are involved HBV and their identification by rational essential approach required to prevent the transmission of this Brazil.To evaluate associated with South Brazil.A total 260 patients controls from Caxias do Sul (state Rio Grande Sul, Brazil) participated study. All participants were given standard questionnaire yield sociodemographic information identify factors. was detected HBsAg test all...

Feline leukemia virus (FeLV) is a retrovirus distributed worldwide in domestic cats and with different outcomes (progressive, regressive, abortive, focal). The present study reports an epidemiological survey of FeLV frequency the evaluation some risk factors two main disease (progressive regressive) urban cat population from Brazil. A total 366 sociodemographic information p27 antigen test performed were included study. DNA (provirus) blood samples all was detected via real-time polymerase...

A reverse transcriptase-polymerase chain reaction (RT-PCR) procedure was used to amplify a VP2 gene fragment (248 bp) from infectious bursal disease virus (IBDV). The allowed the detection of known IBDV strains United States, along with field isolates and commercial vaccines produced in Brazil. Amplified fragments were further characterized by restriction length polymorphism (RFLP) analysis. From 55 Brazilian flocks, 48 samples positive RT-TCR. Vaccine RFLP patterns found 12 pattern...

Mycoplasma gallisepticum (MS) and synoviae are important avian pathogens cause economic losses to the poultry industry. Molecular biology techniques currently used for a rapid detection of these adoption control measures diseases. The aim this study was develop validate technique simultaneous MG MS by multiplex real time polymerase chain reaction (PCR). complete assay (Multiplex MGMS) designed with primers probes specific each pathogen developed be carried out in single tube reaction....

S. Heidelberg is the most frequent serovar in several broiler farms from main Brazilian poultry-producing regions. Therefore, avian-source foods (mainly chicken carcasses) commercialized country and exported to other continents are contaminated with this foodborne pathogen, generating national international economic losses.

Human papillomavirus (HPV) infection is a common viral sexually transmitted and the main cause of cervical cancer in women worldwide.Epidemiological data on prevalence HPV given population essential for establishment effective prevention strategies.The aim this study was to determine who attended public health service within an urban center Brazil.Cervical samples were collected from 337 recruited primary care clinic city Cruz Alta located Rio Grande do Sul, southernmost State Brazil.Samples...

Avian reoviruses (ARVs) can infect a variety of species worldwide. Birds present stunting syndrome, respiratory and/or enteric diseases, immunosuppression, malabsorption, viral arthritis/tenosynovitis, and even secondary infections by other microorganisms. Flaws in conventional vaccines the increase diagnostic rate disease last 5 yr suggest emergence pathogenic ARVs poultry flocks This study aimed to characterize birds lesions detect/genotype from severe outbreak tenosynovitis broiler...

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) pandemic spread rapidly and this scenario is concerning in South America, mainly Brazil that presented more than 21 million coronavirus disease 2019 cases 590 000 deaths. The recent emergence of novel lineages carrying several mutations the spike protein has raised additional public health concerns worldwide. present study describes temporal spreading evolution SARS-CoV2 beginning second wave Brazil, highlighting fast dissemination...