Escherichia coli is a commensal bacterium of the bird's intestinal tract, but it can invade different tissues resulting in systemic symptoms (colibacillosis). This disease occurs only when E. infecting strain presents virulence factors (encoded by specific genes) that enable adhesion and proliferation host organism. Thus, important to differentiate pathogenic (APEC, avian coli) non-pathogenic or fecal (AFEC, isolates. Previous studies analyzed occurrence strains isolated from birds with...

AbstractSalmonella enterica serovar Gallinarum biovar is a pathogenic bacterium that causes fowl typhoid (FT), affecting chicken flocks worldwide. This study aimed to evaluate the emergence, dissemination and genomic profile of S. lineages from Brazil. Twelve whole-genomes sequences (WGS) different strains isolated Brazilian poultry farms (2014 2018) were obtained used construct dataset with other 31 previously published data (five more Brazil). phylogenetic diversity, temporal evolution...

The high prevalence of enterotoxigenic Escherichia coli (ETEC) in nondiarrheic piglets contributes to its rapid spread; however, few studies have explored the effects latent gastrointestinal pathogens on animal health. Therefore, using high‐throughput sequencing approaches, we changes entero‐pulmonary microbiota and immune gene expression healthy, asymptomatic, diarrheic piglets. As expected, bacterial communities were less diverse respiratory tract than gut, with a site‐specific composition...

Pyometra is a life-threatening infectious disease that frequently affects bitches and queens. Although histopathological patterns of pyometra have been extensively explored, the microbiological aspects, such as bacteria pathogenicity, not correlated to microscopy endometrial lesions so far. In this study, these two pathological aspects were analysed correlated. Uterus fragments intrauterine content samples collected from pets diagnosed with (30) submitted histopathology analysis bacterial...

Avian reoviruses (ARVs) can infect a variety of species worldwide. Birds present stunting syndrome, respiratory and/or enteric diseases, immunosuppression, malabsorption, viral arthritis/tenosynovitis, and even secondary infections by other microorganisms. Flaws in conventional vaccines the increase diagnostic rate disease last 5 yr suggest emergence pathogenic ARVs poultry flocks This study aimed to characterize birds lesions detect/genotype from severe outbreak tenosynovitis broiler...

1. Salmonella is one of the most important pathogens in public health and it usually associated with food-borne diseases. serovars Enteritidis Typhimurium are widespread world outbreaks frequently consumption poultry products; furthermore, there an increasing concern wide dissemination serovar Heidelberg flocks. 2. The aim experiment was to develop validate rapid methods detect Enteritidis, Typhimurium, by real-time PCRs test isolates from pre-enriched samples. 3. Three were developed used...

Avian reovirus (ARV) is one of the main causes infectious arthritis/tenosynovitis and malabsorption syndrome (MAS) in poultry. ARVs have been disseminated Brazilian poultry flocks last years. This study aimed to genotype evaluate molecular evolution more frequent ARV lineages detected poultry-producing farms. A total 100 with clinical signs tenosynovitis/MAS, from all regions were positive for by PCR. Seventeen bird tissues submitted cell culture RNA detection/genotyping two PCRs. The...

Salmonella laboratorial detection is usually carried out by bacteriological culture and serological methods. isolates are then classified into more than 2,650 serotypes with different somatic (O) flagellar (H) antigenic combinations. More recently, DNA analysis methods were developed applied for the identification of serotypes, including intergenic spacer regions (ISRs) that separates DNA-encoding ribosomal subunits (rRNA gene) in genomes. The present study aimed to evaluate nucleotide...

Endometrial pathogenic E. coli (EnPEC) isolates are involved in endometrial infections animals and humans. Besides the high aggressiveness of infections, EnPEC virulence profile pathogenesis still little known. In this study, we have sequenced analyzed an strain from canine pyometra (E. coli_LBV005/17), following a molecular characterization phylogenetic evolution collection canines felines (45 strains). Most strains belonged to phylo-group B2, display profile. particular highlight...

Avian pathogenic Escherichia coli (APEC) isolates are currently differentiated from nonpathogenic strains by classical PCR of virulence genes. This study improves the detection five main genes used for APEC with development duplex and single Taqman real-time to these targets. Primers probes targeted ompT, hlyF, iroN, iutA, iss were designed in implementation (iss) (hlyF/ompT iroN/iutA) assays. All E successfully detected (single duplex) PCR. A panel 111 isolates, obtained avian samples...

Bovine genital campylobacteriosis (BGC) is a leading cause of return to estrus in cows. The etiologic agent, Campylobacter fetus subsp. venerealis (Cfv) transmitted by venereal route. Hence, the surrounding reproductive tract microenvironment could play role presence Cfv cervicovaginal mucus Angus breed females provide three experimental groups, which were subject bacteriome analyses: 10 Cfv-positive cows (CVP), Cfv-negative (CVN), and nonsexually active heifers (NSA). Cows with showed...

Hepatitis B virus (HBV) infection is widespread and it considered a major health problem in the world. HBV classified into genotypes subgenotypes. genotype D (HBV-D) has been detected worldwide with high prevalence some specific regions from Europe South America. In Brazil, this very frequent region its introduction dissemination have associated European immigration (mainly Italian). The present study aimed to trace back of HBV-D Brazil. Fifty-two chronic hepatitis patients two cities an...

Abstract Hepatitis B virus (HBV) infection is considered a major health problem in the world. HBV classified into genotypes A to J disseminated worldwide. Genotypes A, D and F are most frequent Western World, C predominant East, E, F, H infrequent restricted specific regions. HBV‐G rare genotype, but it has been detected different continents. This study aimed report temporal evolution global spread of comparing whole‐genome sequences this genotype from regions Bayesian coalescent analysis...

The neuraminidase (NA) genes of A(H1N1)pdm09 influenza virus isolates from 306 infected patients were analysed. circulation oseltamivir-resistant viruses in Brazil has not been reported previously. Clinical samples collected the state Rio Grande do Sul (RS) 2009-2011 and two NA inhibitor-resistant mutants identified, one 2009 (H275Y) other 2011 (S247N). This study revealed a low prevalence resistant (0.8%) with no spread throughout RS.

Capybaras are rodent widely distributed in South America, which inhabit lakeside areas including ecological parks and urban sites. Due to anthropological interaction, monitoring zoonotic pathogens wildlife is essential for One Health. We investigated faecal samples from capybaras living an area Rio Branco (Acre, Brazil) the presence diarrhoeagenic E. coli. Virulence factors shiga toxin-producing coli (STEC), enterohaemorrhagic (EHEC), enteropathogenic (EPEC) were screened by PCR. detected at...

Salmonella is a bacterium usually associated with mild gastroenteritis in chickens, but strains of the serotype Gallinarum can result 2 severe systemic chicken diseases: fowl typhoid (by biovar Gallinarum) and pullorum disease Pullorum). These diseases be controlled by immunization specific vaccine (SG 9R). The aim this study was to characterize recent isolates molecular analyses, as well detect field clinical environmental samples from poultry flocks. Field S. (n = 18) other serotypes 117)...

Poor reproductive performance in beef cattle caused by infectious agents results major financial losses as a result of reduced pregnancy rates and extended calving intervals. Bulls can be subclinical chronic carriers bacterial protozoal involved cow infertility, such Campylobacter fetus subsp. venerealis, Ureaplasma diversum, Mycoplasma bovigenitalium, bovis, Tritrichomonas foetus. harbor these microorganisms their preputial crypts transmit the to cows during natural mating. To obtain an...

INTRODUCTION: Human metapneumovirus (hMPV) is an emergent human respiratory pathogen. This study aimed to evaluate the performance of direct immunofluorescence (DIF) detect hMPV in a clinical laboratory setting. METHODS: Nasopharyngeal aspirate samples (448) children and adults with illness were used by using DIF real time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assays. RESULTS: In all, 36 (8%) positive 94 (21%) qRT-PCR. Direct specificity was 99% sensitivity...

Abstract Bacterial resistance is a public and one health problem. Free‐living birds can be reservoirs of multidrug‐resistant bacteria genes. This study aimed to characterize the antimicrobial Escherichia coli isolated from free‐living urban pigeons ( Columba livia ) in South Brazil. Ninety‐two animals were sampled, isolate was obtained each one. The isolates characterized, profile beta‐lactam colistin genes investigated. classified as phylogroups B1 (35%), B2 (33%), A (16%) D (16%), 14%...

Non-lactose-fermenting Escherichia coli (NLFEC) has a few descriptive studies restricted to human infections. In the present study, isolates of NLFEC obtained from urine samples dogs with hyperadrenocorticism were characterized regarding their virulence ability, biofilm formation capacity and antimicrobial susceptibility profile. lactose-fermenting strains urinary infection in same conditions analysed provide comparisons. The non-lactose-fermenting E. classified as belonging clade I coli,...