A5015166299- Metal complexes synthesis and properties
- RNA and protein synthesis mechanisms
- Synthesis and Characterization of Heterocyclic Compounds
- Bacterial Genetics and Biotechnology
- Synthesis and biological activity
- Trace Elements in Health
- Nanocluster Synthesis and Applications
- Diatoms and Algae Research
- Algal biology and biofuel production
- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- RNA modifications and cancer
- Botanical Research and Chemistry
- X-ray Diffraction in Crystallography
- Crystal structures of chemical compounds
- Biocrusts and Microbial Ecology
- Atmospheric chemistry and aerosols
- Cancer-related gene regulation
- Monoclonal and Polyclonal Antibodies Research
- Genomics and Chromatin Dynamics
- Spectroscopy and Quantum Chemical Studies
- Free Radicals and Antioxidants
- Machine Learning in Bioinformatics
- DNA Repair Mechanisms
- Advanced Chemical Physics Studies
University of Szeged
2018-2024
Recently, we demonstrated that AgI can directly replace ZnII in zinc fingers (ZFs). The cooperative binding of to ZFs leads a thermodynamically irreversible formation silver clusters destroying the native ZF structure. Thus, reported loss biological function proteins is likely consequence such replacement. Here, report an X-ray absorption spectroscopy (XAS) study Agn Sn formed probe their structural features. Selective probing local environment around by XAS showed predominance digonal...
Abstract Zinc finger proteins specifically recognize DNA sequences and, therefore, play a crucial role in living organisms. In this study the Zn(II)-, and DNA-binding of 1MEY#, an artificial zinc protein consisting three units was characterized by multiple methods. Fluorimetric, circular dichroism isothermal calorimetric titrations were applied to determine accurate stability constant protein. Assuming that all subunits behave identically, obtained thermodynamic data for Zn(II) binding ΔH...
We report a comprehensive high-level explicitly correlated ab initio study on the X- + NH2Y [X,Y = F, Cl, Br, I] reactions characterizing stationary points of SN2 (Y- NH2X) and proton-transfer (HX NHY-) pathways as well reaction enthalpies various endothermic additional product channels such H- NHXY, XY- NH2, XY NH2-, XHY- NH. Benchmark structures harmonic vibrational frequencies are obtained at CCSD(T)-F12b/aug-cc-pVTZ(-PP) level theory, followed by CCSD(T)-F12b/aug-cc-pVnZ(-PP) [n Q 5]...
Peptide tags are extensively used for affinity purification of proteins. In an optimal case, these can be completely removed from the purified protein by a specific protease mediated hydrolysis. However, interactions with target may also utilized modulation function. Here we show that C-terminal hexahistidine (6 × His) tag influence catalytic activity nuclease domain Colicin E7 metallonuclease (NColE7) E. coli to kill competing bacteria under stress conditions. This enzyme non-specifically...
Cys2His2 zinc finger proteins are important for living organisms, as they—among other functions—specifically recognise DNA when Zn(II) is coordinated to the proteins, stabilising their ββα secondary structure. Therefore, competition with metal ions may alter original function. Toxic such Cd(II) or Hg(II) might be especially dangerous because of similar chemical properties Zn(II). Most studies carried out so far have involved small peptides. we investigated interactions toxic a consisting...
The (S/T)XH sequence in Cys<sub>2</sub>His<sub>2</sub>zinc fingers can be hydrolytically cleaved by Ni(<sc>ii</sc>) ions. This reaction applied for purification, inhibition or activation of designed zinc finger fusion proteins.
A series of novel estradiol-based salicylaldehyde (thio)semicarbazones ((T)SCs) bearing (O,N,S) and (O,N,O) donor sets their Cu(II) complexes were developed characterized in detail by 1H ¹³C nuclear magnetic resonance spectroscopy, UV–visible electron paramagnetic electrospray ionization mass spectrometry elemental analysis. The structure the Cu(II)-estradiol-semicarbazone complex was revealed X-ray crystallography. Proton dissociation constants ligands stability metal determined 30% (v/v)...
The chirality of the polyether ionophore monensic acid A can be successfully used to study its coordination ability in solution. complementary approach gain new insights into complexation chemistry antibiotic (studied previously by circular dichroism (CD) spectroscopy ultraviolet range (UV-CD)) is presented. (1) Methods: CD visible (VIS-CD) and near-infrared (NIR-CD) applied evaluate affinity deprotonated (monensinate A) towards Ni(II) or Co(II) cations methanolic Competition experiments...
The nuclease domain of colicin E7 cleaves double-strand DNA non-specifically. Zn2+ ion was shown to be coordinated by the purified NColE7 as its native metal ion. Here, we study structural and catalytic aspects interaction with Ni2+, Cu2+ Cd2+ non-endogenous ions consequences their competition ions, using circular dichroism spectroscopy intact protein mass spectrometry. An R447G mutant exerting decreased activity allowed for detection action against pUC119 plasmid via agarose gel...
Abstract Recently, we demonstrated that Ag I can directly replace Zn II in zinc fingers (ZFs). The cooperative binding of to ZFs leads a thermodynamically irreversible formation silver clusters destroying the native ZF structure. Thus, reported loss biological function proteins is likely consequence such replacement. Here, report an X‐ray absorption spectroscopy (XAS) study n S formed probe their structural features. Selective probing local environment around by XAS showed predominance...
Thiosemicarbazones are promising candidates for anticancer therapy and their mechanism of action is often linked to metal chelating ability. In this study, five (thio)semicarbazones with different donor sets (NNS, NNO, ONS, ONO) were selected behaviour in aqueous solution, the stability copper(II) complexes addition cytotoxicity, DNA-binding, DNA cleavage ability inhibition topoisomerase IIα investigated compared. We aimed reveal relationship between structural variations, significantly...