A5041020662- SARS-CoV-2 and COVID-19 Research
- Monoclonal and Polyclonal Antibodies Research
- interferon and immune responses
- Food Allergy and Anaphylaxis Research
- RNA regulation and disease
- Bacteriophages and microbial interactions
- Allergic Rhinitis and Sensitization
- Immune Response and Inflammation
- vaccines and immunoinformatics approaches
- COVID-19 Clinical Research Studies
- Enzyme Structure and Function
- Respiratory viral infections research
- Porphyrin Metabolism and Disorders
- SARS-CoV-2 detection and testing
- Mast cells and histamine
- Glycosylation and Glycoproteins Research
- Neurological diseases and metabolism
- Inflammasome and immune disorders
- Cell Adhesion Molecules Research
- Viral Infections and Immunology Research
- Biochemical and Molecular Research
- HER2/EGFR in Cancer Research
- Kawasaki Disease and Coronary Complications
- Transgenic Plants and Applications
- Microbial Metabolic Engineering and Bioproduction
Jilin University
2025
University of Science and Technology of China
2016-2024
Chinese Academy of Sciences
2016-2022
University of Chinese Academy of Sciences
2022
State Key Laboratory of Transducer Technology
2022
Aerospace Information Research Institute
2022
Hefei National Center for Physical Sciences at Nanoscale
2016-2022
Hefei Institutes of Physical Science
2016
Cellular immunity is important in determining the disease severity of COVID-19 patients. However, current understanding SARS-CoV-2 epitopes mediating cellular limited. Here we apply T-Scan, a recently developed method, to identify CD8+ T cell from patients four major HLA-A alleles. Several identified are conserved across human coronaviruses, which might mediate pre-existing SARS-CoV-2. In addition, and validate that were mutated newly circulating variants, including Delta variant. The...
SARS-CoV-2 variants with adaptive mutations have continued to emerge, causing fresh waves of infection even amongst vaccinated population. The development broad-spectrum antivirals is thus urgently needed. We previously developed two hetero-bivalent nanobodies (Nbs), aRBD-2-5 and aRBD-2-7, potent neutralization activity against the wild-type (WT) Wuhan isolated SARS-CoV-2, by fusing aRBD-2 aRBD-5 aRBD-7, respectively. Here, we resolved crystal structures these Nbs in complex receptor-binding...
Current SARS-CoV-2 Omicron subvariants impose a heavy burden on global health systems by evading immunity from most developed neutralizing antibodies and vaccines. Here, we identified nanobody (aSA3) that strongly cross-reacts with the receptor binding domain (RBD) of both SARS-CoV-1 wild-type (WT) SARS-CoV-2. The dimeric construct aSA3 (aSA3-Fc) tightly binds potently neutralizes WT Based X-ray crystallography, engineered bispecific dimer (2-3-Fc) fusing aSA3-Fc to aRBD-2, previously...
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is still rapidly spreading worldwide. Many drugs and vaccines have been approved for clinical use show efficacy in the treatment prevention of SARS-CoV-2 infections. However, emergence variants concern (VOCs), such as Delta (B.1.617.2) recently emerged Omicron (B.1.1.529), has seriously challenged application current therapeutics. Therefore, there a pressing need identification new broad-spectrum antivirals. Here, we...
The relative pose estimation is one of the most fundamental components for multi-robot systems, while it still remains an open and challenging research topic in infrastructure-free environment. In this letter, we target improving accuracy robustness ground robotic teams, propose to fuse range odometry measurements estimate using sliding window optimization. system, multiple UWB tags ranging are equipped on each robot, visual inertial applied estimating ego-motion robot. Aiming simple...
Summary Autophagy is a recycling process by which eukaryotic cells degrade their own components, and the fruiting body (sexual structure) necessary structure for some plant pathogenic fungi to start infection cycle. However, transcriptional regulation of fungal autophagy regulating sexual reproduction remains elusive. Here, we provide report linking transcription development in phytopathogenic fungi. The forkhead box factor (FOX TF) SsFoxE2 Sclerotinia sclerotiorum (Ss) binds promoters ATG...
Many SARS-CoV-2 neutralizing antibodies (nAbs) lose potency against variants of concern. In this study, we developed 2 strategies to produce mutation-resistant antibodies. First, a yeast library expressing mutant receptor binding domains (RBDs) the spike protein was utilized screen for potent nAbs that are least susceptible viral escape. Among candidate antibodies, P5-22 displayed ultrahigh virus neutralization as well an outstanding mutation resistance profile. Additionally, P14-44 and...
The receptor-binding domain (RBD) of the SARS-CoV-2 spike protein is major target for antibody therapeutics. Shark-derived variable domains new antigen receptors (VNARs) are smallest fragments with flexible paratopes that can recognize motifs inaccessible to classical antibodies. This study reported four VNARs binders (JM-2, JM-5, JM-17, and JM-18) isolated from Chiloscyllium plagiosum immunized RBD. Biolayer interferometry showed bound RBD an affinity KD ranging 38.5 2720 nM, their Fc...
Despite significant discoveries in basic cancer research and improvements treatment options clinical outcomes, remains a major public health concern worldwide. Today, the main focus of is signaling pathways that are crucial for cell survival, proliferation, migration. The aberrant expression proteins involved these often leads to abnormal growth, metastasis, invasion healthy tissues. One such protein discoidin domain receptor 1 (DDR1) which belongs family tyrosine kinases (RTKs) activated...
The structure of allergenic proteins provides important information about the binding allergens to antibodies. In this study, crystal Scy p 4 with a resolution 1.60 Å was obtained by X-ray diffraction. Epitope mapping revealed that linear epitopes are located on surface 4. Also, conformational mostly in structural conservative region. Further comparison, electrostatic potential, and hydrogen bond force analysis showed mutation Asp70 Asp18/20/70 would lead calcium-binding capacity being lost...
Filamin C (FLN c) is a novel allergen in shellfish. In this study, FLN c from Scylla paramamosain was divided into three regions for recombinant expression based on the number of domains and amino acids. Using dot blot basophil activation tests, allergic predominant region determined to be 336-531 acid positions (named c-M). It confirmed that by X-ray diffraction, crystal structure c-M with immunoglobulin-like folding at resolution 1.7 Å obtained. The monomer barrel composed 16 β-strands 2...
Thiolases are vital enzymes which participate in both degradative and biosynthetic pathways. Biosynthetic thiolases catalyze carbon–carbon bond formation by a Claisen condensation reaction. The cytoplasmic acetoacetyl-CoA thiolase from Saccharomyces cerevisiae , ERG10, catalyses the mevalonate pathway. structure of S. has not previously been reported. Here, crystal structures apo ERG10 its Cys91Ala variant were solved at resolutions 2.2 1.95 Å, respectively. determined shows that shares...
The NLRP1 inflammasome functions as canonical cytosolic sensor in response to intracellular infections and is implicated auto-inflammatory diseases. But the regulation signal transduction mechanisms of are incompletely understood. Here, we show that T60 variant CARD8, but not T48 isoform, negatively regulates activation by directly interacting with receptor molecule inhibiting assembly. Furthermore, our results suggest different ASC preference three types inflammasomes, namely...
Arginine kinase (AK) from the phosphagen family is a cross-reactive shellfish allergen. Structurally related allergens are involved in pathogenesis of allergic symptoms. This study aimed to unravel cross-reactivity AK structural perspective. The crystal structure