A5013708422- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Reproductive Physiology in Livestock
- Animal Genetics and Reproduction
- Ovarian function and disorders
- Fish biology, ecology, and behavior
- Aquaculture Nutrition and Growth
- Effects of Environmental Stressors on Livestock
- Reproductive biology and impacts on aquatic species
- Renal and related cancers
- Environmental Toxicology and Ecotoxicology
- Fish Biology and Ecology Studies
- Marine Biology and Environmental Chemistry
- Lipid metabolism and biosynthesis
- Soil Management and Crop Yield
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Ichthyology and Marine Biology
- CRISPR and Genetic Engineering
- Reproductive Health and Technologies
- Rural Development and Agriculture
- Pluripotent Stem Cells Research
- Bee Products Chemical Analysis
- Tissue Engineering and Regenerative Medicine
- Research in Cotton Cultivation
- Assisted Reproductive Technology and Twin Pregnancy
Universidade Federal do Pará
2013-2025
Universidade Federal Rural da Amazônia
2014
Altera (United States)
2009
Amazon Research Foundation
2009
Abstract Anti-Müllerian Hormone (AMH), a member of the TGF-β superfamily, plays critical role in mammalian fertility. This study aimed to investigate AMH concentrations pregnant buffalo cows and their fetuses, characterize cellular distribution immunolocalization within fetal gonads. Gonads were collected from 12 male 14 female fixed 10% buffered formalin, processed for routine histological analysis immunohistochemical localization AMH. Blood samples 26 corresponding fetuses into EDTA-coated...
In vitro-produced embryos store high lipid content in cytoplasmic droplets (LD), and reduction or removal of LD has been demonstrated to improve freeze-thaw viability. The Perilipin Adipophilin Tail-interacting Protein 47 kD (PAT) family proteins is involved the formation regulation many cell types, but their presence not addressed either cattle oocytes preimplantation embryos. Therefore, this study aimed detect expression PAT transcripts (Perilipin-2 [PLIN2] Perilipin-3 [PLIN3]) immature...
Nitric oxide (NO) is a cell-signaling molecule that regulates variety of molecular pathways. We investigated the role NO during preimplantation embryonic development by blocking its production with an inhibitor or supplementing in vitro bovine embryo cultures natural precursor, L-arginine, over different periods. Endpoints evaluated included blastocyst rates, kinetics, and quality. Supplementation synthase N-Nitro-L-arginine-methyl ester (L-NAME) from Days 1 to 8 culture decreased (P < 0.05)...
Abstract The in vivo fertilization process occurs the presence of follicular fluid (FF). aim this study was to evaluate effect vitro medium supplementation with 5% or 10% bovine (BFF) on production embryos. FF collected from ovarian follicles a diameter 8–10 mm, and cumulus–oocyte complexes (COCs) were co‐incubated sperm for 24 h commercial BotuFIV® (BotuPharma©), being distributed among experimental groups: oocytes fertilized control medium; supplemented BFF; BFF. After fertilization,...
One of the crucial aspects to be considered for successful in vitro production (IVP) embryos is composition various media used throughout stages this reproductive biotechnology. The cell culture employed should fulfill metabolic requirements both gametes during oocyte maturation and sperm development, as well embryo its initial divisions. Most IVP protocols incorporate blood serum into a source hormones, proteins, growth factors, nutrients. Numerous studies have suggested Platelet-Rich...
In this study, histological, ultrastructural, and immunohistochemical observations of the gill tissue two fish species (Plagioscion squamosissimus Lithodoras dorsalis) were used as biomarkers to assess ecological conditions an estuary in Amazon delta, by relating health status fishes environmental quality. The was analyzed both qualitatively semi-quantitatively. Marked differences type severity lesions verified between among study sites. A variety severe damages confirmed histologically...
This work analyzes the morphological alterations in Brachyplatystoma rousseauxii gills and evaluates whether this species can be considered an environmental monitoring biomarker of Guajará Bay. Sampling was carried out four areas around Belém, Brazil, annual periods: dry/wet season, wet wet/dry season dry season. Water pH, temperature suspended material were evaluated. A total 36 specimens collected. The second right gill arch each animal removed immediately fixed processed for...
Somatic cell nuclear transfer (SCNT) success is partially hindered by the low epigenetic reprogramming efficiency of donor cell. Previous studies suggest cellular heterogeneity among nuclei in regard to potential, which precludes comparison different strategies increase cloning success. In this context, we evaluated effect using clonal populations (CPs) bovine adult fibroblasts established single-cell plating SCNT. Different CPs were proliferation rate, senescence level, and chromosome...
To evaluate the Adipose Stem Cells (ACS) therapy efficacy on time and quality of wound healing process in rats.Nine male Wistar rats were randomly distributed into three groups I) 7 days healing; II) 14 III) 21 healing. Four incisions made dorsal surface each rat then treated with intralesional ACS, meloxicam, no treatment ACS+meloxicam. Macroscopic evaluation was measured by percentage histopathological hematoxylin-eosin performed.All have reduced during weeks (p<0.001) after had greater...
Ruminant energy supplementation with vegetable oils or fats has been standing out worldwide and oil palm processing receiving growing interest. This study assessed the effect of saturated unsaturated fatty acids from industry on lipid profile seminal plasma sperm membrane, as well morphological functional characteristics raw cryopreserved buffalo semen. Twelve purebred Murrah bulls (Bubalus bubalis) were assigned to experimental groups fed diets for 120 days no added lipids (CONT, four...
Summary Sex selection through sperm sorting offers advantages in regards pressure high-producing livestock. However, the sex-sorting process results membrane and DNA damage that ultimately decrease fertility. We hypothesized given role of protamines packaging, protamine deficiency could account, at least partially, for observed following sex sorting. To test this, we compared status between unsexed sexed spermatozoa from two bulls using fluorochrome chromomycin A3 (CMA3) flow cytometry....
Com o objetivo de avaliar a cinética da maturação in vitro oócitos bubalinos, foram cultivados 1.619 com cumulus oophorus compacto em quatro diferentes tratamentos: T1 -TCM 199 10% Soro Fetal Bovino (SFB) (meio base); T2 -Meio base e células granulosa (CG); T3 base, CG, 10 UI gonadotrofina coriônica equina (eCG) humana (hCG); T4 eCG, 10UI hCG 1 µg 17b-estradiol / ml, tempos 14 17h, 20 22h, 23 25h, 26 28h, 29h 32h cultivo estufa CO 2 5% temperatura 38,5ºC.No tratamento 1, observou-se que...
Abstract In this longitudinal study, the anti‐Müllerian hormone (AMH) levels in blood were determined 32 Murrah buffalo females at 8, 10, 12, 16 and 19 months of age when synchronized antral follicular population (AFP) was estimated. Correlations AFP to AMH level retrospectively younger ages investigated. Then split into high low AFP, their compared for all tested as predictors categories. The highest ( p < .05) detected 8 months, reducing but not differing > 12 then again age. mean...
In reproductive technologies, uncovering the molecular aspects of oocyte and embryo competence under different conditions is crucial for refining protocols enhancing efficiency. RNA-seq generates high-throughput data provides transcriptomes that can undergo additional computational analyses. This study presented transcriptomic profiles
Despite the cytotoxicity and embryotoxicity previously reported artesunate is a recommended drug to treat malaria for adults, children, women in first trimester of pregnancy. To address putative effects on female fertility preimplantation embryo development, when pregnancy not detectable yet, was added oocyte
Aim of study: To evaluate the response dermal fibroblasts to heat stress and different time exposures on cell survival gene expression. Area Belém city, Pará state. Brazil. Material methods: Fibroblasts were isolated from ear skin bovine (n= 4) buffalo 4), cultured in vitro until 3rd passage submitted at 42°C for 3, 6 12 h, except negative control (38.5°C 24 h). Cell was measured using Trypan blue, RNA isolation performed Trizol method following qRT-PCR quantify relative expression inducible...