A5039671866- Nanopore and Nanochannel Transport Studies
- Advanced biosensing and bioanalysis techniques
- Microfluidic and Capillary Electrophoresis Applications
- Ion-surface interactions and analysis
- Bacteriophages and microbial interactions
- RNA Interference and Gene Delivery
- Antimicrobial Peptides and Activities
- Lipid Membrane Structure and Behavior
- Advanced Electron Microscopy Techniques and Applications
- Fuel Cells and Related Materials
- Parkinson's Disease Mechanisms and Treatments
- Alzheimer's disease research and treatments
- Advancements in Transdermal Drug Delivery
- Mass Spectrometry Techniques and Applications
- Advanced Biosensing Techniques and Applications
- Botulinum Toxin and Related Neurological Disorders
École Polytechnique Fédérale de Lausanne
2022-2023
Technical University of Munich
2023
Bioengineering Center
2023
Klinikum rechts der Isar
2023
Adolphe Merkle Institute
2017-2021
University of Fribourg
2017-2021
KU Leuven
2019
University of Applied Sciences Biberach
2017
Protein post-translational modifications (PTMs) play a crucial role in countless biological processes, profoundly modulating protein properties on both spatial and temporal scales. PTMs have also emerged as reliable biomarkers for several diseases. However, only handful of techniques are available to accurately measure their levels, capture complexity at single molecule level, characterize multifaceted roles health disease. Nanopore sensing provides high sensitivity the detection...
Parkinson's disease (PD), multiple system atrophy (MSA), and dementia with Lewy bodies (DLB) are neurodegenerative disorders alpha-synuclein (α-syn) aggregation pathology. Different strains of α-syn unique properties suggested to cause distinct clinical pathological manifestations resulting in PD, MSA, or DLB. To study individual spreading patterns, we injected fibrils amplified from brain homogenates two MSA patients PD into the brains C57BI6/J mice. Antibody staining against pS129-α-syn...
Protein pores recently enabled a breakthrough in bioanalytics by making it possible to sequence individual DNA and RNA strands during their translocation through the lumen of pore. Despite this success overall promise nanopore-based single-molecule analytics, protein have not yet reached full potential for analysis characterization globular biomolecules such as natively folded proteins. One reason is that diameters available are too small accommodating most proteins lumen. The work presented...
MelP5 is a 26 amino acid peptide derived from melittin, the main active constituent of bee venom, with five replacements. The pore-forming activity in lipid membranes attracting attention because forms larger pores and induces dye leakage through liposome at lower concentration than melittin. Studies have so far focused on ensemble measurements membrane impedance; here we extend this characterization an electrophysiological comparison between melittin using planar bilayer recordings. These...
Pore-forming antimicrobial peptides (AMPs) are attracting interest as cytolytic antibiotics and drug delivery agents with potential use for targeting cancer cells or multidrug-resistant pathogens. Ceratotoxin A (CtxA) is an insect-derived AMP 36 amino acids that thought to protect the eggs of medfly Ceratitis capitata against Single channel recordings using planar lipid bilayers have shown CtxA forms pores well-defined conductance states resembling those alamethicin; it also one largest...
β-barrel nanopores are involved in crucial biological processes, from ATP export mitochondria to bacterial resistance, and represent a promising platform for emerging sequencing technologies. However, the fundamental principles governing ion transport across these remain poorly understood due their extreme confinement heterogeneous lumen charge. In this study, we integrate experimental, numerical, theoretical approaches elucidate mechanisms nanopores. We identify characterise two distinct...
Abstract Protein post-translational modifications (PTMs) play a crucial role in countless biological processes, profoundly modulating protein properties on both the spatial and temporal scales. PTMs have also emerged as reliable biomarkers for several diseases. However, only handful of techniques are available to accurately measure their levels, capture complexity at single molecule level characterize multifaceted roles health disease. Nanopore sensing provides high sensitivity detection...
Abstract High resolution fluorescence microscopy is a key tool in the elucidation of biological fine-structure, providing insights into distribution and interactions biomolecular systems down to nanometer scale. Expansion recently developed approach achieving nanoscale optical imaging. In experiment, samples are embedded hydrogel, which isotropicaly swollen. This physically pulls labels apart, allowing more them be resolved. However, gelation swelling process, two factors combine reduce...
Abstract Nanopores are one of the most successful label-free single-molecule techniques with several sensing applications such as biological screening, diagnostics, DNA and protein sequencing 1–4 . In current nanopore technologies, stochastic processes influence both selection translocating molecule, translocation rate velocity 5,6 As a result, translocations difficult to control spatially temporally. Here we present novel method where engineer precise spatial temporal into experiment. We...