The results of work by several investigators indicate that crossbridge attachment serves as a positive feedback mechanism transiently increases the Ca2+ affinity troponin C (TnC) during each normal heartbeat. To monitor structural changes in cardiac isoform TnC (cTnC) associated with binding and muscle, we labeled cTnC sulfhydryl-specific fluorescent probe 2-(4'-iodoacetamidoanilino)naphthalene-6-sulfonic acid (IAANS). When IAANS-labeled (cTnCIAANS) was substituted for endogenous TnC,...

The purpose of this study was to evaluate the early outcomes percutaneous microwave ablation (MWA) for clinical stage T1 (cT1) renal masses when performed within a high-volume practice with critical emphasis on procedural safety.A retrospective review registry identified 26 patients total 27 cT1 treated MWA between 2011 and 2017. Mean patient age 63.8 years 16 (61.5%) were male. mass size ± SD 2.3 0.8 cm (range, 1.1-4.7 cm). main outcome parameters investigated technical success, local tumor...

To evaluate the association between renal tumour complexity and outcomes in a large cohort of patients undergoing percutaneous cryoablation (PCA).Patients with tumours treated PCA were identified using our prospectively maintained ablation registry (2003-2015). Salvage procedures inherited syndromes excluded. The associations R.E.N.A.L. nephrometry score (NS) risk complications, function impairment, local failure cancer-specific mortality (CSM) evaluated univariate multivariable logistic,...

Background The neonatal myocardium is more sensitive to volatile anesthetics compared with adults. greater myocardial sensitivity of neonates may be attributable anesthetic effect on force regulation at the level cross-bridge. In current study, authors effects 1 and 2 minimum alveolar concentration (MAC) halothane sevoflurane cardiac muscle from 0- 3-day-old (neonate) 84-day-old (adult) rats. Methods Triton X-100-skinned strips were maximally activated pCa (negative logarithm Ca2+...

Background The surface membrane Ca(2+)-adenosine triphosphatase and Na(+)-Ca(2+) exchanger transport Ca(2+) out of the ventricular myocyte, competing for cytosolic with located in sarcoplasmic reticulum. In this study authors examined effects halothane, isoflurane, sevoflurane on extrusion from cell reticulum content. Methods Single myocytes right free wall adult male ferret hearts were isolated, loaded acetoxymethyl ester fluorescent indicator fluo-3, electrically stimulated at 0.25 Hz to...

Tracheobronchial disruption is an uncommon but severe complication of double lumen endotracheal tube placement. The physical properties a (large external diameter and length) make tracheobronchial injury more common than that associated with smaller single tubes. Here we present the case iatrogenic left main bronchus caused by placement in otherwise unremarkable airway.

Retrospective cohort study.This study compares time to extubation between major spine surgery patients anesthetized with fentanyl versus sufentanil infusions in combination propofol.In undergoing spinal fusion intraoperative electrophysiological monitoring of the cord, total intravenous anesthesia a propofol/opioid results better signals compared volatile anesthetics. Pharmacokinetic data suggest that should lead more rapid emergence from than fentanyl. However, this has never been tested...

Prader-Willi syndrome (PW) is a rare genetic disorder with multi-organ system involvement. These patients present many perioperative challenges including sleep-related breathing disorders, morbid obesity, thick salivary secretions, mental retardation, and difficult intravenous access. PW has been suggested to be associated central adrenal insufficiency. We report novel case of persistent severe hypotension from previously undiagnosed asymptomatic insufficiency in pediatric patient during...

Background Isoflurane depresses the intracellular Ca2+ transient and force development during a twitch, but its effects on crossbridge cycling rates are difficult to predict because of nature twitch. Measurements isoflurane kinetics tetanic contractions, which provide steady state level activation in intact cardiac muscle, have not been previously reported. Methods Ferret right ventricular papillary muscles were isolated, superficial cells microinjected with bioluminescent photoprotein...

We tested the hypothesis that force-velocity history modulates thin filament activation, as assessed by rate of force redevelopment after shortening (+dF/dt(R)). The influence isotonic on +dF/dt(R) was imposing uniform amplitude (2.55 to 2.15 microm sarcomere(-1)) but different speed releases intact frog muscle fibres during fused tetani. Each release consisted a contiguous ramp- and step-change in length. Ramp changed from vary fibre 1.00 (2.76 +/- 0.11 half-sarcomere(-1) s(-1)) 0.30...

Background Depression of myocardial contractility as a result isoflurane appears to be greater in hypertrophy, and the cellular basis for this difference susceptibility is not clear. In study we examined effects sevoflurane on intracellular calcium an animal model pressure-overload hypertrophy. Methods Pressure-overload hypertrophy was established young male ferrets by banding main pulmonary artery 1 month ([Ca]i) were isolated right ventricular papillary muscles, trabeculae, myocytes....

Some of isoflurane's cellular actions, such as interference with intracellular Ca(2+) handling, inhibition the respiratory chain, and capability to produce oxygen radicals, could result in impaired function during ischemia/reoxygenation (I/R). We investigated effects isoflurane applied I/R on Ca(2+), radical formation, arrhythmic events, contractile rat cardiomyocytes. Single ventricular myocytes were subjected 30 min simulated ischemia followed by reoxygenation. After baseline measurements,...

Although hypercholesterolemia is a strong risk factor for cardiovascular disease, it has in some instances paradoxically been associated with reduced infarct size and preserved contractile function isolated hearts after ischemia reperfusion. To elucidate potential cellular protective mechanisms, myocytes of hypercholesterolemic apolipoprotein E-deficient (ApoE-/-) wild-type mice were subjected to hypoxic metabolic inhibition (I) subsequent reoxygenation (R). Intracellular Ca2+ concentration...

Background: Isoflurane applied before myocardial ischemia has a beneficial preconditioning effect which involves generation of reactive oxygen species (ROS); ROS, however, have been implicated in critical cytosolic calcium ([Ca 2+ ] i ) overload during ischemia. We therefore investigated isoflurane's effects on intracellular Ca handling ischemic ventricular myocytes and the association with ROS. Methods: Simulated was induced electrically stimulated rat for 30 min (ischemia)....

A 42 year-old male sustained an accidental rifle gunshot wound to his left foot, resulting in fracture deformities of the calcaneus, navicular, cuneiform, 1st and 2nd metatarsal bases, talus. As he was transported our trauma center, developed progressive encephalopathy. Urgent external fixator placement under general anesthesia postponed due encephalopathy unknown etiology. Brain magnetic resonance imaging demonstrated a “starfield” pattern infarcts, consistent with cerebral fat embolism...