Submarine Groundwater Discharge (SGD) is an important yet poorly recognised pathway of material transport to the marine environment. This work reports on results dissolved inorganic carbon (DIC) and organic (DOC) concentrations loads in groundwater seeping into southern Baltic Sea. Most research was carried out Bay Puck (2009–2010), while 2013 study extended include several other seepage impacted areas situated along Polish coastline. The annual average DIC DOC were equal 64.5 ± 10.0 mg C L−...

Klebsiella pneumoniae and K. oxytoca are members of Enterobacteriaceae. They Gram-negative, non-motile rods that ubiquitous in the environment part human intestinal microbiota. These opportunistic pathogens may cause pneumonia urinary tract infections. species genetically biochemically similar; therefore, it is important to find reliable methods for their differentiation. This study presents results biochemical assays, PCR, MALDI-TOF mass spectrometry (MS) performed on 35 isolates obtained...

Organic carbon deposited in marine sediments is an important part of the global cycle. The knowledge concerning role shelf seas (including Baltic Sea) cycle has increased substantially, however organic accumulation rates still require clarification. This paper describes methods used for assessing and nitrogen six sediment cores collected areas Sea. Mass were based on 210Pb method validated by 137Cs measurements. ranged from 18 to 75 g·C·m−2·yr−1. C/N ratios δ13C access sedimentary matter...

Ficolins are serum pattern recognition molecules. They have opsonic properties and able to activate complement via the lectin pathway. This paper reports investigations concerning ficolin-2 ficolin-3 in ovarian cancer (OC). Their levels, single nucleotide polymorphisms of corresponding FCN2 FCN3 genes specific mRNA expression sections were investigated 128 patients suffering from primary OC 197 controls operated on for reasons other than malignancies. The latter consisted two reference...

We analyse recent Mediterranean Sea surface temperatures (SSTs) and their response to global change using 1/4-degree gridded advanced very-high-resolution radiometer (AVHRR) daily SST data, 1982SST -2012.These data indicate significant annual warming (from 0.24 • C decade -1 west of the Strait Gibraltar 0.51 over Black Sea) spatial variation in average 15 21 Levantine sub-basin).Ensemble mean scenarios that study area may experience warming, peaking at 2.6 century Representative...

Klebsiella pneumoniae is considered one of the most critical multidrug-resistant pathogens and urgently requires new therapeutic strategies. Capsular polysaccharides (CPS), lipopolysaccharides (LPS), exopolysaccharides (EPS) are major virulence factors protecting K. against immune response thus may be targeted by phage-based therapeutics such as polysaccharides-degrading enzymes. Since emergence resistance to antibacterials generally undesirable, in this study, genetic phenotypic...

Ficolin-1 (M), ficolin-2 (L), ficolin-3 (H) and mannan-binding lectin (MBL) activate the complement system have opsonic activity. The specificity of is poorly characterized currently limited to a few ligands only. We present new specific targets for human ficolin-3, identified among lipopolysaccharides (LPSs, endotoxin) Hafnia alvei. interaction was restricted LPSs four strains: 23, Polish Collection Microorganisms (PCM) 1200, PCM 1203 1205 their O-specific polysaccharides (O-specific PSs)...

Organic substances are important components of the marine environment as they determine properties seawater and key biogeochemical processes taking place in it. carbon (OC) is a measure organic matter. For practical purposes, OC divided into dissolved (DOC) particulate (POC). Both DOC POC play major role cycle, especially shelf seas like Baltic, where their concentrations substantial. In three-year study (2009–2011) samples for measurements were collected from stations located Gdańsk Deep,...

Enterobacterial common antigen (ECA) is a conserved surface characteristic for Enterobacteriaceae. It consisting of trisaccharide repeating unit, →3)-α-d-Fucp4NAc-(1→4)-β-d-ManpNAcA-(1→4)-α-d-GlcpNAc-(1→, where prevailing forms include ECA linked to phosphatidylglycerol (ECAPG) and cyclic (ECACYC). Lipopolysaccharide (LPS)-associated form (ECALPS) has been proved date only rough Shigella sonnei phase II. Depending on the structure organization, constitutes (ECAPG ECALPS) or maintains outer...

Lipopolysaccharide (LPS, endotoxin), the main surface antigen and virulence factor of Gram-negative bacteria, is composed lipid A, core oligosaccharide O-specific polysaccharide regions. Each LPS region capable complement activation. We have demonstrated that Hafnia alvei, an opportunistic human pathogen, reacts strongly with murine mannose-binding lectins (MBL). Moreover, MBL-LPS interactions were detected for majority other species investigated. H. alvei was used as a model pathogen to...

This study is a continuation of the research on organic matter sources, distribution and dynamics in southern Baltic Sea described detail by Maciejewska Pempkowiak (2014). In this paper, cluster analysis, principal component analysis segment were used to assess relations among factors influencing concentration sea-water. The following sea-water properties, salinity (Sal), temperature (Temp), pH, concentrations chlorophyll (Chla) phaeopigment (Feo), assessed, while dissolved (DOC) particulate...

This paper discusses predictions of particulate organic carbon (POC) concentrations in the southern Baltic Sea. The study is based on one-dimensional Particulate Organic Carbon Model (1D POC), described detail by Dzierzbicka-Głowacka et al. (2010a). POC concentration determined as sum phytoplankton, zoo-plankton and dead matter (detritus) concentrations. Temporal changes phytoplankton biomass are caused primary production, mortality, grazing zooplankton sinking. affected ingestion,...

Klebsiella pneumoniae is a nosocomial pathogen, pointed out by the World Helth Organisation (WHO) as “critical” regarding highly limited options of treatment. Lipopolysaccharide (LPS, O-antigen) and capsular polysaccharide (K-antigen) are its virulence factors surface antigens, determining O- K-serotypes encoded or K-loci. They promising targets for antibody-based therapies (vaccines passive immunization) an alternative to antibiotics. To make such immunotherapy effective, knowledge about...

The herein presented complete structure of the core oligosaccharide lipopolysaccharide (LPS) P. shigelloides Polish Collection Microorganisms (PCM) 2231 (serotype O17) was investigated by 1H, 13C NMR spectroscopy, mass spectrometry, chemical analyses and serological methods. is composed an undecasaccharide, which represents second type identified for serotype O17 LPS. This similar to that strains 302-73 O1) 7-63 differs from these only one sugar residue. Serological screening 55 with use...

The impact of planktonic and biofilm lifestyles the clinical isolate Proteus mirabilis 9B-m on its lipopolysaccharide (O-polysaccharide, core region, lipid A) was evaluated. bacteria are able to form is one factors involved in formation. Lipopolysaccharide isolated from cells investigated strain analyzed by SDS–PAGE with silver staining, Western blotting ELISA, as well NMR matrix-assisted laser desorption ionization time-of-flight mass spectrometry techniques. Chemical spectroscopic analyses...

Non-metastatic pancreatic ductal adenocarcinoma (PDAC) is classified as resectable (R), borderline (BR) or locally advanced (LA). International Consensus Guidelines on these definitions exist, but have not been integrated into everyday Australian practice. The anatomical features CT imaging lend themselves to synoptic reporting which should enhance completeness, comparability and consistency.We developed tested a report for PDAC derived from the at two metropolitan cancer services...

Abstract. This paper presents various scenarios of the particulate organic carbon (POC) in southern Baltic Sea. The study is based on a one-dimensional Particulate Organic Carbon model (1-D POC). Mathematically, pelagic variables 1-D POC are described by second-order partial differential equations diffusion type with biogeochemical sources and sinks. concentration determined as sum phytoplankton, zooplankton dead matter (detritus) concentrations. temporal changes phytoplankton biomass caused...

Abstract. Submarine Groundwater Discharge (SGD) is an important, yet poorly recognized pathway of material transport to the marine environment. This work reports on results dissolved inorganic carbon (DIC) and organic (DOC) concentrations in groundwater seeping Bay Puck. The loads via SGD were quantified for Baltic Sea sub-basins entire Sea. annual averages DIC DOC equal 64.5 ± 10.0 mg C L−1 5.8 0.9 L−1. fluxes estimated at 283.6 66.7 kt yr−1 25.5 4.2 yr−1. derived load important component...

Most Proteus lipopolysaccharides (LPSs) contain uronic acids or their amides with different amino acids, which together other negatively charged components account for the acidic character of such LPS molecules. Previous studies have shown significance an amide galacturonic acid lysine [D-GalA(L-Lys)] serological specificity O-antigens from few P. mirabilis strains. In this work, immunodominant role GalALys was indicated penneri 42 core region. The also showed identity oligosaccharides...