Glycosaminoglycans (GAGs) are important sulfated carbohydrates prevalent in the extracellular matrix. The synthesis of structurally defined GAGs requires laborious procedures, and incorporating sulfation patterns is challenging. automated chondroitin hexasaccharides on solid support has been achieved using a photolabile linker that efficiently cleaved continuous-flow photoreactor. As service to our authors readers, this journal provides supporting information supplied by authors. Such...

Abstract Multivalent mannose‐functionalized nanoparticles self‐assembled from amphiphilic β‐cyclodextrins (β‐CDs) facilitate the targeted delivery of anticancer drugs to specific cancer cells. Doxorubicin (DOX)‐loaded equipped with multivalent mannose target units were efficiently taken up via receptor‐mediated endocytosis by MDA‐MB‐231 breast cells that overexpress receptor. Upon entering cell, intracellular pH causes release DOX, which triggers apoptosis. Targeting significantly improved...

The lack of cancer cell specificity and the occurrence multidrug resistance (MDR) are two major obstacles in treatment hepatocellular carcinoma (HCC). To tackle these challenges, a novel nanoparticle (NP)-based drug delivery system (DDS) with core/shell structure consisted d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS)-galactose (Gal)/polydopamine (PDA) is fabricated. NP loaded doxorubicin (DOX) nitric oxide (NO) donor

MicroRNAs are small highly conserved noncoding RNAs that widely expressed in multicellular organisms and participate the regulation of various cellular processes including autophagy viral replication. Evidently, microRNAs able to modulate host gene expression thereby inhibit or enhance hepatitis B virus (HBV) The miR-99 family members liver. Interestingly, plasma levels peripheral blood correspond with HBV DNA loads. Thus, we asked whether regulated replication analyzed underlying molecular...

The targeted delivery of a photosensitizer (PS) with appropriate carriers represents an attractive means selectively delivering cargo to target tissues or subcellular compartments for photodynamic therapy (PDT). Herein, three-arm distyryl BODIPY derivative conjugated mannose units (denoted by BTM) that can co-assemble Tween 80 form nanomicelles (BTM-NMs) PDT is reported. MDA-MB-231 breast cancer cells recognized and specifically internalized BTM-NMs via mannose-receptor-mediated endocytosis...

A novel stepwise and regioselective nucleophilic aromatic substitution (SNAr) reaction of halogenated boron dipyrrins (BODIPYs) with pyrroles has been developed under mild conditions no catalyst needed shown to be diversity oriented. The resultant pyrrole-substituted BODIPYs are interesting red near-infrared (NIR) fluorescent dyes absorption maxima up 733 nm. Removal the BF2 protecting group 3-pyrrole or 3,5-dipyrrole-substituted provides a facial entry oligopyrroles direct 2,2′-bipyrrole linkages.

The targeted delivery of a photosensitizer (PS) into specific cancer cells is an effective way to enhance the efficacy and minimize side effects photodynamic therapy. Herein, heptamannosylated β-cyclodextrin (β-CD) was used mediate formation adamantane (Ad)-functionalized BODIPY PS nanoparticle via strong β-CD/Ad complexation. mannose-functionalized nanoparticles are selectively internalized by mannose-receptor-rich MDA-MB-231 breast receptor-mediated endocytosis, facilitating singlet oxygen...

Plesiomonas shigelloides, a pathogen responsible for frequent outbreaks of severe travelers' diarrhea, causes grave extraintestinal infections. Sepsis and meningitis due to P. shigelloides are associated with high mortality rate as antibiotic resistance increases vaccines not available. Carbohydrate antigens expressed by pathogens often structurally unique targets developing diagnostics. Here, we report total synthesis the highly functionalized trisaccharide repeating unit 2 from serotype 51...

Abstract Hepatocellular carcinoma (HCC) causes high morbidity and mortality due to a lack of adequate treatments. Cancer treatments have benefited from nanotechnology approaches that integrate multimodal synergistic therapies. A synergistic, minimally invasive strategy interventional photodynamic therapy (IPDT) chemotherapy for HCC treatment through percutaneous transperitoneal puncture is disclosed based on photosensitive porphyrinic galactose‐modified metal‐organic frameworks (PCN‐224)...

Acinetobacter baumannii is a opportunistic bacterial pathogen responsible for serious nosocomial infections that becoming increasingly resistant against antibiotics. Capsular polysaccharides (CPS) cover A. are major virulence factor play an important role in pathogenesis, used to assign serotypes and provide the basis vaccine development. Synthetic oligosaccharides resembling CPS of 17978 were printed onto microarray slides screen sera from patients infected with as well monoclonal mouse...

Abstract Nanomaterials hold promise for the treatment of human carcinomas but integrating multiple functions into a single drug carrier system remains challenging. Herein, an integrated therapeutic delivery hepatocellular carcinoma (HCC) is reported, which based on rhodamine B (RhB) end‐labeled cationic poly[2‐(dimethylamino)ethyl methacrylate] (PDMAEMA) and hydrophobic poly(3‐azido‐2‐hydroxypropyl methacrylate) (PGMA‐N 3 ) segments equipped with covalently bound galactose. This...

The development of glycoconjugate vaccines against Helicobacter pylori is challenging. An exact epitope the H. lipo-polysaccharide (LPS) O-antigens that contain Lewis determinant oligosaccharides and unique dd-heptoglycans has not yet been identified. Reported here first total synthesis serotype O6 tridecasaccharide O-antigen containing a terminal Ley tetrasaccharide, α-(1→3)-, α-(1→6)-, α-(1→2)-linked heptoglycan, β-d-galactose connector, by an [(2×1)+(3+8)] assembly sequence. Seven...

A nanomicelles-based and lactose-decorated drug delivery system (DDS) is fabricated loaded with a small molecule Triapine photosensitizer Ce6 to afford TCLMs. TCLMs highly hepatocyte-targeted due the specific recognition of lactose by human asialoglycoprotein receptor (ASGPR) collapses in acidic microenvironment cancer cells release cargos. The employment allows combined chemo-photodynamic therapy (CT-PDT), which both act synergistically as boosters cytotoxic reactive oxygen species (ROS)...

Acinetobacter baumannii is currently posing a serious threat to global health. Lipopolysaccharide (LPS) potent virulence factor of pathogenic Gram-negative bacteria. To explore the antigenic properties A. LPS, four Kdo-containing inner core glycans from strain ATCC 17904 were synthesized. A flexible and divergent method based on use orthogonally substituted α-Kdo-(2→5)-Kdo disaccharides was developed. Selective removal different protecting groups in these key precursors elongation sugar...

Helicobacter pylori, listed as a human carcinogen by the Department of Health and Human Services, colonizes gastric mucosa more than half world's population. The individuals infected with H. pylori have high risk to develop chronic gastritis, peptic ulcers, even cancer. conserved core structure lipopolysaccharide (LPS) has been regarded promising candidate for development glycoconjugate vaccine targeting multiple serotypes. Here, we report total synthesis undecasaccharide LPS its subunit...

Pseudomonas aeruginosa bacteria are becoming increasingly resistant against multiple antibiotics. Therefore, the development of vaccines to prevent infections with these is an urgent medical need. While immunological activity lipopolysaccharide O-antigens in P. well-known, specific protective epitopes remain unidentified. Herein, we present first chemical synthesis highly functionalized aminoglycoside trisaccharide 1 and its acetamido derivative 2 found serotype O5 O-antigen. The targets...

5-Aminolevulinic acid synthase (ALAS) is the key rate-limiting enzyme in synthesis of vital biosynthetic intermediate 5-aminolevulinic (ALA). However, its catalytic efficiency compromised due to low activity and poor stability. Here, we obtained mutant I325M/V390Y/H391I (T6), which exhibited a 7.0-fold increase specific (2.53 U/mg) compared wild type through application isothermal compressibility (βT) perturbation engineering conjunction with two thermal stability prediction algorithms....