A5075876792- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Polyamine Metabolism and Applications
- Fermentation and Sensory Analysis
- Probiotics and Fermented Foods
- Microbial metabolism and enzyme function
- Advanced biosensing and bioanalysis techniques
- Biofuel production and bioconversion
- Microbial Metabolism and Applications
- Amino Acid Enzymes and Metabolism
- Biochemical Analysis and Sensing Techniques
- Analytical Chemistry and Chromatography
- Enzyme Production and Characterization
- Algal biology and biofuel production
- Proteins in Food Systems
- Innovative Microfluidic and Catalytic Techniques Innovation
- Microfluidic and Capillary Electrophoresis Applications
- Gout, Hyperuricemia, Uric Acid
- Enzyme Structure and Function
- Biosensors and Analytical Detection
- Fungal Biology and Applications
- biodegradable polymer synthesis and properties
- Pharmacological Effects of Natural Compounds
- Steroid Chemistry and Biochemistry
- Polymer Science and PVC
Tianjin University of Science and Technology
2024-2025
Jiangnan University
2016-2025
Key Laboratory of Guangdong Province
2025
Institute of Food Science & Technology
2025
State Key Laboratory of Food Science and Technology
2020
Harvard University
2015
Bentham Science Publishers (United Arab Emirates)
2014
Bentham Science Publishers (China)
2014
Shanghai Jiao Tong University
2013
South China University of Technology
2008-2009
Aptamers, functional nucleic acids recognized for their high target-binding affinity and specificity, have been extensively employed in biosensors, diagnostics, therapeutics. Conventional screening methods apply evolutionary pressure to optimize affinity, while counter-selections are used minimize off-target binding improve specificity. However, aptamer specificity characterization remains limited target analogs experimental controls. A systematic exploration of the chemical space...
Protein-glutaminase plays a significant role in future food (e.g., plant-based meat) processing as result of its ability to improve the solubility, foaming, emulsifying, and gel properties proteins. However, poor stability, activity, high pressure, shear environments hinder application. Therefore, we developed an application-oriented method isothermal compressibility perturbation engineering strategy enzyme performance by simulating high-pressure environment. The best variant with remarkable...
β-1,4-Endoxylanase is the most critical hydrolase for xylan degradation during lignocellulosic biomass utilization. However, its poor stability and activity in hot alkaline environments hinder widespread application. In this study, BhS7Xyl from Bacillus halodurans S7 was improved using a computer-aided design through isothermal compressibility (βT) perturbation engineering by combining three thermostability prediction algorithms (ICPE-TPA). The best variant with remarkable improvement...
The utilization of amine-negative starter based on an understanding nitrogen metabolism is a useful method for controlling biogenic amine (BA) in Chinese rice wine (CRW) fermentation. contribution brewing materials to protein degradation was analyzed; wheat Qu had no effect, and yeast autolysis generated 10% amino nitrogen. Milling degree strongly correlated with BAs formation ( R2 = 0.99). Subsequently, Lactobacillus plantarum Staphylococcus xylosus were coinoculated as at optimized ratio...
Cavities are created by hydrophobic interactions between residue side chain atoms during the folding of enzymes. Redesigning cavities can improve thermostability and catalytic activity enzyme; however, synergistic effect remains unclear. In this study, Rhizomucor miehei lipase (RML) was used as a model to explore volume fluctuation spatial distribution changes internal cavities, which could reveal roles in activity. We present an inside out cavity engineering (CE) strategy based on...
The O-glycosylation of polyphenols for the synthesis glycosides has garnered substantial attention in food research applications. However, practical utility UDP-glycosyltransferases (UGTs) is significantly hindered by their low catalytic efficiency and suboptimal regioselectivity. concurrent optimization regioselectivity activity during glycosylation presents a formidable challenge. Here, we addressed long-standing activity–regioselectivity tradeoff glycosyltransferase UGTBL1 through...
Abstract Background Surfactin, a green lipopeptide bio-surfactant, exhibits excellent surface, hemolytic, antibacterial, and emulsifying activities. However, lack of clear understanding the synthesis regulation mechanism surfactin homologue components has hindered customized production products with different biological Results In this study, exogenous valine 2-methylbutyric acid supplementation significantly facilitated C14–C15 proportions (up to 75% or more), positive correlation between...
5-Aminolevulinic acid synthase (ALAS) is the key rate-limiting enzyme in synthesis of vital biosynthetic intermediate 5-aminolevulinic (ALA). However, its catalytic efficiency compromised due to low activity and poor stability. Here, we obtained mutant I325M/V390Y/H391I (T6), which exhibited a 7.0-fold increase specific (2.53 U/mg) compared wild type through application isothermal compressibility (βT) perturbation engineering conjunction with two thermal stability prediction algorithms....