A5030858876- Digital Holography and Microscopy
- Optical measurement and interference techniques
- Advanced Fluorescence Microscopy Techniques
- Microfluidic and Bio-sensing Technologies
- Optical Coherence Tomography Applications
- Cell Image Analysis Techniques
- Advanced Optical Imaging Technologies
- Advanced X-ray Imaging Techniques
- Image Processing Techniques and Applications
- Orbital Angular Momentum in Optics
- Reproductive Biology and Fertility
- Photoacoustic and Ultrasonic Imaging
- Sperm and Testicular Function
- Spectroscopy Techniques in Biomedical and Chemical Research
- Near-Field Optical Microscopy
- Physics of Superconductivity and Magnetism
- Advanced Vision and Imaging
- Bladder and Urothelial Cancer Treatments
- Cellular Mechanics and Interactions
- Photonic and Optical Devices
- Optical Network Technologies
- Thermography and Photoacoustic Techniques
- Photorefractive and Nonlinear Optics
- Frequency Control in Power Systems
- AI in cancer detection
Tel Aviv University
2016-2025
Duke University
2009-2011
Ben-Gurion University of the Negev
2005-2011
Johns Hopkins University
2009
Bar-Ilan University
1998-2004
Soreq Nuclear Research Center
1993-2002
Israel Atomic Energy Commission
1996
We present a new tomographic phase microscopy (TPM) approach that allows capturing the three-dimensional refractive index structure of single cells in suspension without labeling, using 180° rotation cells. This is obtained by integrating an external off-axis interferometer for wide-field wave front acquisition with holographic optical tweezers (HOTs) trapping and micro-rotation suspended In contrast to existing TPM approaches cell imaging, our does not require anchoring sample rotating...
Abstract Label‐free super‐resolution (LFSR) imaging relies on light‐scattering processes in nanoscale objects without a need for fluorescent (FL) staining required super‐resolved FL microscopy. The objectives of this Roadmap are to present comprehensive vision the developments, state‐of‐the‐art field, and discuss resolution boundaries hurdles that be overcome break classical diffraction limit label‐free imaging. scope spans from advanced interference detection techniques, where...
Carbon quantum dots (CQDs) are one of the most promising types fluorescent nanomaterials due to their exceptional water solubility, excellent optical properties, biocompatibility, chemical inertness, refractive index, and photostability. Nitrogen-containing CQDs, which include amino acid based especially attractive high yield, thermal stability, potential biomedical applications. Recent studies have attempted improve preparation CQDs. However, highest yield obtained for these was only 44%....
This Letter presents the τ interferometer, a portable and inexpensive device for obtaining spatial interferograms of microscopic biological samples without strict stability highly coherent illumination that are usually required interferometric microscopy setups. The is built using off-the-shelf optical elements can easily operate with low-coherence illumination, while being positioned in output conventional inverted microscope. processed into quantitative amplitude phase profiles...
We present analysis tools which are formulated using wide-field interferometric phase microscopy measurements, and show their ability to uniquely quantify the life cycle of live cancer cells. These parameters based directly on optical path delay profile sample do not necessitate decoupling refractive index thickness in cell profile, thus can be calculated a single-frame acquisition. To demonstrate use these parameters, we have constructed setup closely traced full lifecycle HeLa initial...
We present a simple-to-align, highly-portable interferometer, which is able to capture wide-field, off-axis interference patterns from transparent samples under low-coherence illumination. This small-dimensions and low-cost device can be connected the output of transmission microscope illuminated by source measure sub-nanometric optical thickness changes in label-free manner. In contrast our previously published design, τ new design fully operate an holographic geometry, where fringes have...
We present a phase-shifting interferometric technique for imaging live biological cells in growth media, while optimizing spatial resolution and enabling potential real-time measurement capabilities. The uses slightly-off-axis interferometry which requires less detector bandwidth than traditional off-axis fewer measurements on-axis interferometry. Experimental theoretical comparisons between the proposed method these approaches are given. is experimentally demonstrated via phase microscopy...
We introduce and experimentally demonstrate a fast accurate method for quantitative imaging of the dynamics live biological cells. Using dual-channel interferometric setup, two phase-shifted interferograms nearly transparent samples are acquired in single digital camera exposure digitally processed into phase profile sample. Since same sample simultaneously, most common noise is eliminated, enabling visualization millisecond-scale dynamic phenomena with subnanometer optical path length...
We present a new interferometric and holographic approach, named interferometry with doubled imaging area (IDIA), which it is possible to double the camera field of view while performing off-axis imaging, without changing parameters, such as magnification resolution. This technique enables quantitative amplitude phase wider samples reducing acquisition frame rate due scanning. The method implemented using compact module that connects regular digital camera, useful in wide range applications...
A major challenge in the field of optical imaging live cells is achieving rapid, 3D, and noninvasive isolated without labeling. If successful, many clinical procedures involving analysis sorting drawn from body fluids, including blood, can be significantly improved. new label‐free tomographic interferometry approach presented. This provides rapid capturing 3D refractive‐index distribution single suspension. The flow a microfluidic channel, are trapped, then rapidly rotated by...
We present cytometric classification of live healthy and cancer cells by using the spatial morphological textural information found in label-free quantitative phase images cells. compare both to primary tumor cell metastatic cells, where biopsies normal tissues were isolated from same individuals. To mimic analysis liquid flow cytometry, imaged while unattached substrate. used low-coherence off-axis interferometric microscopy setup, which allows a single-exposure acquisition mode, thus is...
Abstract Currently, the delicate process of selecting sperm cells to be used for in vitro fertilization (IVF) is still based on subjective, qualitative analysis experienced clinicians using non‐quantitative optical microscopy techniques. In this work, a method was developed automated quantitative phase maps acquired through use interferometric (IPM). Over 1,400 human from 8 donors were imaged IPM, and an algorithm designed digitally isolate cell heads while taking into consideration both 3D...
Off-axis holographic multiplexing involves capturing several complex wavefronts, each encoded into off-axis holograms with different interference fringe orientations, simultaneously, a single camera acquisition. Thus, the multiplexed hologram can capture wavefronts at once, where one encodes information from sample, using same number of pixels typically required for acquiring conventional encoding only sample wavefront. This gives rise to many possible applications, focus on acquisition...
Many medical and biological protocols for analyzing individual cells involve morphological evaluation based on cell staining, designed to enhance imaging contrast enable clinicians biologists differentiate between various organelles. However, staining is not always allowed in certain procedures. In other cases, may be time-consuming or expensive implement. Staining operator-sensitive, hence lead varying analytical results, as well cause artificial artifacts false heterogeneity. We present a...
We present a method for real time visualization and automatic processing detection classification of untouched cancer cells in blood during stain free imaging flow cytometry using digital holographic microscopy machine learning throughput 15 per second. As preliminary model circulating tumor blood, we automatically classified primary metastatic colon cells, where the two types were isolated from same individual, as well four cells. used low-coherence off-axis interferometric phase...
Methods of generating multiple viewpoint projection holograms three-dimensional (3-D) realistic objects illuminated by incoherent white light are reviewed in this paper. Using these methods, it is possible to obtain with a simple digital camera, operating regular conditions. Thus, most disadvantages characterizing conventional holography, namely the need for powerful, highly coherent laser and extreme stability optical system, avoided. The proposed holographic processes composed two stages....
We apply wide-field interferometric microscopy techniques to acquire quantitative phase profiles of ventricular cardiomyocytes in vitro during their rapid contraction with high temporal and spatial resolution.The whole-cell are analyzed yield valuable parameters characterizing the cell dynamics, without need decouple thickness from refractive index differences.Our experimental results verify that these new can be used wide field discriminate modulation cardiomyocyte dynamics due temperature...
We present a new approach for predicting spatial phase signals originating from photothermally excited metallic nanoparticles of arbitrary shapes and sizes. The heat emitted such nanoparticle affects the measured optical signal via changes in both refractive index thickness surroundings. Because these particles can be bio-functionalized to bind certain biological cell components, they used biomedical imaging with molecular specificity, as nanoscopy labels, photothermal therapy. Predicting...
We present efficient algorithms for rapid reconstruction of quantitative phase maps from off-axis digital holograms. The new are aimed at speeding up the conventional Fourier-based algorithm. By implementing on a standard personal computer, while using only single-core processing unit, we were able to reconstruct unwrapped one megapixel holograms frame rates 45 frames per second (fps). When unwrapping is not required, same allow 150 fps In addition obtaining real-time visualization sample,...
We present a portable, off-axis interferometric module for quantitative phase microscopy of live cells, positioned at the exit port coherently illuminated inverted microscope. The creates on digital camera an interference pattern between image sample and its flipped version. proposed simplified is based retro-reflector modification in external Michelson interferometer. does not contain any lenses, pinholes, or gratings alignment straightforward. Still, it allows full control angle suffer...
We present a new approach for obtaining significant speedup in the digital processing of extracting unwrapped phase profiles from off-axis holograms. The technique digitally multiplexes two orthogonal holograms, where reconstruction, including spatial filtering and two-dimensional unwrapping on decreased number pixels, can be performed both holograms together, without redundant operations. Using this technique, we were able to reconstruct, first time our knowledge, with 1 megapixel more than...