A5091817878- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- RNA Research and Splicing
- Insect Resistance and Genetics
- Bacterial Genetics and Biotechnology
- Carbohydrate Chemistry and Synthesis
- Enzyme Structure and Function
- Natural Compound Pharmacology Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Bioactive Compounds and Antitumor Agents
- DNA and Nucleic Acid Chemistry
University of Michigan
2016-2024
Pentatricopeptide repeat (PPR) motifs are α-helical structures known for their modular recognition of single-stranded RNA sequences with each motif in a tandem array binding to single nucleotide. Protein-only RNase P 1 (PRORP1) Arabidopsis thaliana is an endoribonuclease that uses its PPR domain recognize precursor tRNAs (pre-tRNAs) as it catalyzes removal the 5'-leader sequence from pre-tRNAs NYN metallonuclease domain. To gain insight into mechanism by which PRORP1 recognizes tRNA, we...
Protein-only ribonuclease P (PRORP) is an enzyme responsible for catalyzing the 5′ end maturation of precursor transfer ribonucleic acids (pre-tRNAs) encoded by various cellular compartments in many eukaryotes. PRORPs from plants act as single-subunit enzymes and have been used a model system analyzing function metazoan PRORP nuclease subunit, which requires two additional proteins efficient catalysis. There are currently few molecular details known about PRORP–pre-tRNA complex. Here, we...
Abstract Protein‐only RNase P (PRORP) is an essential enzyme responsible for the 5′ maturation of precursor tRNAs (pre‐tRNAs). PRORPs are classified into three categories with unique molecular architectures, although all classes share a mechanism and have similar active sites. Single subunit PRORPs, like those found in plants, multiple isoforms different localizations, substrate specificities, temperature sensitivities. Most recently, Arabidopsis thaliana PRORP2 was shown to interact TRM1A...
Abstract The first step in transfer RNA (tRNA) maturation is the cleavage of 5’ end precursor (pre-tRNA) catalyzed by ribonuclease P (RNase P). RNase either a ribonucleoprotein (RNP) complex with catalytic subunit or pro tein-only R Nase (PRORP). In most land plants, algae, and Euglenozoa, PRORP single-subunit enzyme. There are currently no inhibitors protein-only that can be used as tools for studying biological function this Therefore, we screened compounds inhibit activity model from A....