A5056210598- Immune cells in cancer
- Zebrafish Biomedical Research Applications
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Antibiotics Pharmacokinetics and Efficacy
- Drug-Induced Adverse Reactions
- Reproductive System and Pregnancy
- Pregnancy and preeclampsia studies
- Blood disorders and treatments
- Carcinogens and Genotoxicity Assessment
- Mesenchymal stem cell research
- Eosinophilic Esophagitis
- Caveolin-1 and cellular processes
- Hematopoietic Stem Cell Transplantation
- Infant Nutrition and Health
- Advanced Glycation End Products research
- Inflammation biomarkers and pathways
- Vitamin K Research Studies
- Phagocytosis and Immune Regulation
- Cellular Mechanics and Interactions
- Single-cell and spatial transcriptomics
- Venomous Animal Envenomation and Studies
- Immunotherapy and Immune Responses
- Angiogenesis and VEGF in Cancer
MRC Centre for Regenerative Medicine
2016-2024
University of Edinburgh
2010-2024
Centre for Inflammation Research
2010-2014
Medical Research Council
2014
Queen's Medical Centre
2010-2013
The Queen's Medical Research Institute
2012
Wake Forest University
1990
Atrium Health Wake Forest Baptist
1990
A prostaglandin barrier to inflammation Blood-borne bacterial infections and severe trauma can send the immune system into overdrive, causing it pump out inflammatory mediators, sometimes at lethal doses. Duffin et al. now report on a role for prostaglandins in keeping systemic check. Systemic correlates with decreased production of E2 (PGE2). Blocking PGE2 signaling mice led associated translocation gut bacteria. acts innate lymphoid cells, which produce interleukin-22, secreted protein...
Hematopoietic stem cells (HSCs) first emerge in the embryonic aorta-gonad-mesonephros (AGM) region. Studies of model organisms defined intersecting signaling pathways that converge to promote HSC emergence predominantly ventral domain dorsal aorta. Much less is known about mechanisms driving development humans. Here, identify secreted signals underlying human development, we combined spatial transcriptomics analysis dorsoventral polarized aorta with gene expression profiling sorted cell...
Hematopoietic stem cells (HSCs) produce all essential cellular components of the blood. Stromal cell lines supporting HSCs follow a vascular smooth muscle (vSMC) differentiation pathway, suggesting that some hematopoiesis-supporting originate from vSMC precursors. These pericyte-like precursors were recently identified in aorta-gonad-mesonephros (AGM) region; however, their role hematopoietic development vivo remains unknown. Here, we identify subpopulation NG2
The technical limitations of isolating neutrophils without contaminating leukocytes, while concurrently minimizing neutrophil activation, is a barrier to determining specific functions. We aimed assess the use FACS for generating highly pure quiescent populations in an antibody-free environment. Peripheral blood human granulocytes and murine bone marrow-derived were isolated by discontinuous Percoll gradient flow-sorted using FSC/SSC profiles differences autofluorescence. Postsort purity was...
Hematopoietic stem cell (HSC) generation in the aorta-gonad-mesonephros region requires HSC specification signals from surrounding microenvironment. In zebrafish, PDGF-B/PDGFRβ signaling controls hematopoietic stem/progenitor (HSPC) and is required niche. Little known about murine HSPC vivo whether involved. Here, we show that PDGFRβ expressed distinct perivascular stromal layers mid-gestation dorsal aorta, its deletion impairs hematopoiesis. We demonstrate PDGFRβ+ cells play a dual role...
The co-ordinated recruitment of monocyte subpopulations, neutrophils and regulatory T-cells (Tregs) during the early stages human acute lung inflammation remains poorly understood. We therefore performed a detailed characterisation these lineages in blood lungs model inflammation. Healthy volunteers inhaled lipopolysaccharide (LPS) or saline (n=6 for each group). Blood was collected at 0, 2, 4, 6 8 h bronchoscopy with bronchoalveolar lavage (BAL) h. Multiparameter flow cytometry used to...
The genotoxic potential of methylglyoxal (MG) was studied in Saccharomyces cerevisiae D7 and Salmonella typhimurium TA97 TA102 the presence absence metabolic activation system (S9 fraction) prepared from mouse liver induced with beta-naphthoflavone (beta-NF) sodium phenobarbital (PB). vivo effects on hepatic microsomal mixed function mono-oxygenase by MG were untreated, beta-NF or PB pre-treated mice. a direct-acting mutagen S. when tested up to maximum concentration 0.47 mg/plate. Mitotic...
We have previously reported the presence of novel subpopulations pulmonary monocyte-like cells (PMLC) in human lung; resident PMLC (rPMLC, HLA-DR+CD14++CD16+cells) and inducible (iPMLC, HLA-DR+CD14++CD16- cells). iPMLC are significantly increased bronchoalveolar lavage (BAL) fluid following inhalation lipopolysaccharide (LPS). carried out first functional evaluation inflamed lung, isolation these cells, other lineages, from BAL using complex protocols. iPMLC, rPMLC, alveolar macrophages...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of quaternary amine ether lipids and evaluation neoplastic cell growth inhibitory propertiesSusan L. Morris-Natschke, Karen Meyer, Canio J. Marasco Jr., Claude Piantadosi, Fiona Rossi, Patrick Godwin, Edward ModestCite this: Med. Chem. 1990, 33, 6, 1812–1818Publication Date (Print):June 1, 1990Publication History Published online1 May 2002Published inissue 1 June...
The cellular cytoskeleton, adhesion receptors, extracellular matrix composition, and their spatial distribution are together fundamental in a cell's balanced mechanical sensing of its environment. We show that, lung injury, matrix-integrin interactions altered this leads to signalling alteration missensing. missensing, secondary cell surface receptor alterations, increased stiffness, death. have identified monoclonal antibody against β1 integrin which caused remodelling enhancement survival....
An osteoblast-like human osteosarcoma cell line (U2-OS) has been shown to possess a vitamin K-dependent carboxylation system which is similar the in HepG2 cells and liver lung from rat. In an ‘in vitro’ prepared these cells, K1 was overcome warfarin inhibition of gamma-carboxylation carried out by carboxylase. The data suggest that osteoblasts, involved synthesis proteins bone, can use as antidote poisoning if enough accumulate tissue. Five precursors were identified respectively. microsomes...
Flow cytometric analysis and flow cell sorting (FACS) have revolutionized the understanding of immune biology proved an indispensible tool not just for immunologists but those interested in inflammatory processes. Right through from early days impedance-based cytometry counter devices to first fluorescence-based instruments where side forward scatter parameters with one or two florescent color state-of-the-art multicolour mass over 38-antibody panels, we are discovering new types multiple...
<h3>Introduction</h3> Acute lung injury (ALI) has a mortality rate of over 30%, with no proven pharmacological treatment. Inhalation lipopolysaccharide (LPS) in healthy volunteers induces transient inflammation resembling that found patients ALI. Inhaled LPS causes neutrophilia is detectable bronchoalveolar lavage fluid (BALF) and blood, but its effect on BALF blood monocyte populations not well established. <h3>Methods</h3> 12 were recruited randomly allocated to receive either 60 μg...
AbstractEffects on host defenses of Total Parenteral Nutrition (TPN) with long-(LCT) and medium-chain triglycerides (MCT) were studied.Survival to lipopolysaccharide (LPS) challenge, blood clearance Escherichia coli, in vivo vitro production tumor necrosis factor-α (TNF-α) interleukin-6 (IL-6) investigated.In BALB/c mice, LCTs produced a 25% reduction mortality, compared controls. TPN performed LCT plus MCT mixture reduced mortality by 50%. Spasms appeared after 18 h 12 respectively mice...