A5084090737- Epigenetics and DNA Methylation
- Steroid Chemistry and Biochemistry
- Protist diversity and phylogeny
- Fungal and yeast genetics research
- Genomics and Chromatin Dynamics
- Plant-Microbe Interactions and Immunity
- Pesticide and Herbicide Environmental Studies
- Plant Molecular Biology Research
- RNA modifications and cancer
- Fungal Biology and Applications
- Antifungal resistance and susceptibility
- Polysaccharides and Plant Cell Walls
- DNA Repair Mechanisms
- Microbial Metabolism and Applications
- Fungal Plant Pathogen Control
- Cellular transport and secretion
- Mycotoxins in Agriculture and Food
- Plant Pathogens and Fungal Diseases
- Plant Disease Resistance and Genetics
University of Georgia
2016-2021
Toyo University
2012-2017
Significance Polycomb Repressive Complex 2 (PRC2) methylates histones to regulate multicellular development, maintenance of stem cell identity, X-chromosome inactivation, and other important processes. Given these essential roles, there is significant interest in identifying components that function with PRC2 establish maintain transcriptionally repressive heterochromatin. Here we document an unexpected new role for a well-studied conserved chromatin remodeling factor, ISWI. We found the...
LSH/DDM1 enzymes are required for DNA methylation in higher eukaryotes and have poorly defined roles genome maintenance yeast, plants, animals. The filamentous fungus Neurospora crassa is a tractable system that encodes single homolog (NCU06306). We report the enzyme encoded by mutagen sensitive-30 (mus-30), locus identified genetic screen over 25 years ago. show MUS-30-deficient cells normal methylation, but hypersensitive to damaging agents. MUS-30 nuclear protein, consistent with its...
Abstract Histone H1 variants, known as linker histones, are essential chromatin components in higher eukaryotes, yet compared to the core histones relatively little is about their vivo functions. The filamentous fungus Neurospora crassa encodes a single protein that not for viability. To investigate role of N. H1, we constructed functional FLAG-tagged fusion and performed genomic molecular analyses. Cell fractionation experiments showed H1-3XFLAG binding protein....
contains a minimal Polycomb repression system, which provides rich opportunities to explore Polycomb-mediated across eukaryotes and enables genetic studies that can be difficult in plant animal systems. Repressive Complex 2 is multi-subunit complex deposits mono-, di-, trimethyl groups on lysine 27 of histone H3, H3K27 molecular marker transcriptionally repressed facultative heterochromatin. In mouse embryonic stem cells multiple species, H2A.Z has been found colocalized with methylation....
Regulation of chromatin accessibility and transcription are tightly coordinated processes. Studies in yeast higher eukaryotes have described accessible regions, but little work has been done filamentous fungi.Here we present a genome-scale characterization regions Neurospora crassa, which revealed characteristic molecular features inaccessible chromatin. We experimental evidence inaccessibility within heterochromatin Neurospora, examine both chromatin, including the presence histone...
Among three MAPK disruptants of Neurospora crassa, Δmak-1 was sensitive and Δmak-2 hypersensitive to micafungin, a beta-1,3-glucan synthase inhibitor, than the wild-type or Δos-2 strains. We identified six micafungin-inducible genes that are involved in cell wall integrity (CWI) found MAK-1 regulated transcription non-anchored protein gene, ncw-1, beta-1,3-endoglucanase bgt-2, whereas MAK-2 controlled expression glycosylhydrolase-like gh76-5, C4-dicarboxylate transporter tdt-1. Western...
ABSTRACT Neurospora crassa contains a minimal Polycomb repression system, which provides rich opportunities to explore Polycomb-mediated across eukaryotes and enables genetic studies that can be difficult in plant animal systems. Repressive Complex 2 is multi-subunit complex deposits mono-, di-, tri-methyl groups on lysine 27 of histone H3, H3K27 molecular marker transcriptionally repressed facultative heterochromatin. In mouse embryonic stem cells multiple species, H2A.Z has been found...
ABSTRACT Histone H1 variants, known as linker histones, are essential chromatin components in higher eukaryotes, yet compared to the core histones relatively little is about their vivo functions. The filamentous fungus Neurospora crassa encodes a single protein that not for viability. To investigate role of N. H1, we constructed functional FLAG-tagged fusion and performed genomic molecular analyses. Cell fractionation experiments showed H1-FLAG binding protein. Chromatin-immunoprecipitation...