A5002740468- Steroid Chemistry and Biochemistry
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- Protist diversity and phylogeny
- Pesticide and Herbicide Environmental Studies
- Fungal and yeast genetics research
- Plant Molecular Biology Research
- RNA modifications and cancer
- Genomics and Phylogenetic Studies
- Fungal Infections and Studies
- DNA Repair Mechanisms
- Histone Deacetylase Inhibitors Research
- Nematode management and characterization studies
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Plant Disease Resistance and Genetics
- Plant and fungal interactions
University of Georgia
2018-2021
Southern Connecticut State University
2015
Abstract Polycomb group (PcG) proteins form chromatin modifying complexes that stably repress lineage- or context-specific genes in animals, plants, and some fungi. Repressive Complex 2 (PRC2) catalyzes trimethylation of lysine 27 on histone H3 (H3K27me3) to assemble repressive chromatin. In the model fungus Neurospora crassa, H3K27me3 deposition is controlled by H3K36 methyltransferase ASH1 components constitutive heterochromatin including H3K9me3-binding protein HETEROCHROMATIN PROTEIN 1...
Significance Polycomb Repressive Complex 2 (PRC2) methylates histones to regulate multicellular development, maintenance of stem cell identity, X-chromosome inactivation, and other important processes. Given these essential roles, there is significant interest in identifying components that function with PRC2 establish maintain transcriptionally repressive heterochromatin. Here we document an unexpected new role for a well-studied conserved chromatin remodeling factor, ISWI. We found the...
Abstract Cellular differentiation is instructed by developmental regulators in coordination with chromatin remodeling complexes. Much information about their comes from studies the model ascomycetous yeasts. It not clear, however, what kind of that can be extrapolated to species other phyla Kingdom Fungi. In basidiomycete Cryptococcus neoformans , transcription factor Znf2 controls yeast-to-hypha differentiation. Through a forward genetic screen, we identified basidiomycete-specific Brf1. We...
contains a minimal Polycomb repression system, which provides rich opportunities to explore Polycomb-mediated across eukaryotes and enables genetic studies that can be difficult in plant animal systems. Repressive Complex 2 is multi-subunit complex deposits mono-, di-, trimethyl groups on lysine 27 of histone H3, H3K27 molecular marker transcriptionally repressed facultative heterochromatin. In mouse embryonic stem cells multiple species, H2A.Z has been found colocalized with methylation....
Regulation of chromatin accessibility and transcription are tightly coordinated processes. Studies in yeast higher eukaryotes have described accessible regions, but little work has been done filamentous fungi.Here we present a genome-scale characterization regions Neurospora crassa, which revealed characteristic molecular features inaccessible chromatin. We experimental evidence inaccessibility within heterochromatin Neurospora, examine both chromatin, including the presence histone...
ABSTRACT Neurospora crassa contains a minimal Polycomb repression system, which provides rich opportunities to explore Polycomb-mediated across eukaryotes and enables genetic studies that can be difficult in plant animal systems. Repressive Complex 2 is multi-subunit complex deposits mono-, di-, tri-methyl groups on lysine 27 of histone H3, H3K27 molecular marker transcriptionally repressed facultative heterochromatin. In mouse embryonic stem cells multiple species, H2A.Z has been found...