A5025665668- Gut microbiota and health
- Pancreatic function and diabetes
- Diabetes and associated disorders
- Diabetes Management and Research
- Clostridium difficile and Clostridium perfringens research
- Diet and metabolism studies
- Diet, Metabolism, and Disease
- Bacteriophages and microbial interactions
- Lipoproteins and Cardiovascular Health
- Genetic factors in colorectal cancer
- Nutrition, Genetics, and Disease
- Diabetes Management and Education
- Helicobacter pylori-related gastroenterology studies
- RNA and protein synthesis mechanisms
- Epigenetics and DNA Methylation
- SARS-CoV-2 detection and testing
- Advanced Proteomics Techniques and Applications
- Food Security and Health in Diverse Populations
University of Amsterdam
2020-2024
Amsterdam University Medical Centers
2020-2024
Eindhoven University of Technology
2021
Abstract Background The occurrence of metabolic syndrome (MetS) and the gut microbiota composition are known to differ across ethnicities yet how these three factors interwoven is unknown. Also, it unknown what relative contribution each MetS component whether this differs between ethnicities. We therefore determined its components in multi-ethnic HELIUS cohort tested overall ethnic-specific associations with composition. Methods included 16,209 treatment naïve participants study, which were...
Objective Although gut dysbiosis is increasingly recognised as a pathophysiological component of metabolic syndrome (MetS), the role and mode action specific microbes in health remain elusive. Previously, we identified commensal butyrogenic Anaerobutyricum soehngenii to be associated with improved insulin sensitivity subjects MetS. In this proof-of-concept study, investigated potential therapeutic effects A. L2-7 on systemic responses duodenal transcriptome profiles individuals Design...
It is currently unknown whether associations between gut microbiota composition and type 2 diabetes (T2D) differ according to the ethnic background of individuals. Thus, we studied these in participants from two ethnicities characterized by a high T2D prevalence living same geographical area, using Healthy Life In Urban Settings (HELIUS) study. We included 111 128 on metformin (Met-T2D), 78 49 treatment-naïve (TN-T2D) participants, as well 1:1 matched group healthy controls from,...
Use of genetic risk scores (GRS) may help to distinguish between type 1 diabetes and 2 diabetes, but less is known about whether GRS are associated with disease severity or progression after diagnosis. Therefore, we tested residual beta cell function glycaemic control in individuals diabetes.
Hyperglycemia and type 2 diabetes (T2D) are caused by failure of pancreatic beta cells. The role the gut microbiota in T2D has been studied, but causal links remain enigmatic. Obese individuals with or without were included from two independent Dutch cohorts. Human data translated vitro vivo using islets C57BL6/J mice injecting flagellin into obese mice. Flagellin is part bacterial locomotor appendage flagellum, present bacteria including Enterobacteriaceae, which we show to be more abundant...
Background and aimsFamilial hypercholesterolemia (FH) is caused by pathogenic variants in LDLR, APOB, or PCSK9 genes (designated FH+). However, a significant number of clinical FH patients do not carry these FH-). Here, we investigated whether intronic regions LDLR attribute to affecting pre-mRNA splicing.MethodsLDLR introns are partly covered routine sequencing using next-generation sequencing. Deep variants, >20 bp from intron-exon boundary, were considered interest once (a) present FH- (n...
Shotgun proteomics is a generic method enabling detection of multiple viral species in one assay. The reliable and accurate identification these by analyzing peptides from MS-spectra challenging task. aim this study was to develop an easy accessible proteome analysis approach for the viruses that cause respiratory gastrointestinal infections. For purpose, shotgun based web application, 'proteome2virus', were developed. Identified searched database comprising proteomic data 46 known be...
The gut microbiota constitutes an important modulator of metabolic heath and unbalanced is increasingly recognized as risk factor for disorders, such obesity, insulin resistance type 2 diabetes. Seeking novel treatments to counteract disturbances in obese subjects, we explore whether delivering a protective commensal bacteria (Anaerobutyricum soehngenii L2-7) directly into the small intestine treatment-naïve subjects with syndrome would ameliorate glucose metabolism (NTR NL6630). We...
Abstract Objective Hyperglycemia and type 2 diabetes (T2D) are caused by failure of pancreatic beta cells. The role the gut microbiota in T2D has been studied but causal links remain enigmatic. Design Obese individuals with or without were included from two independent Dutch cohorts. Human data was translated vitro vivo using islets C57BL6/J mice injecting flagellin into obese mice. Results Flagellin is part bacterial locomotor appendage flagellum, present on bacteria including...